Effect of aluminium-induced Alzheimer like condition on oxidative energy metabolism in rat liver, brain and heart mitochondria

Cyril V. Swegert, Kunjan R Dave, Surendra S. Katyare

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Prolonged exposure of rats to aluminium (Al) can result in an Alzheimer- like condition. To get better insights into the biochemical defects underlying AD, senility and ageing we exposed rats for long durations (90-100 days) to soluble salt of aluminium (AlCl3) and checked its influence on mitochondrial respiratory activity in the liver, brain and heart. In the liver and brain mitochondria the ADP/O ratio was impaired with NAD+ linked substrates. State three respiration decreased with glutamate in the liver. For succinate, the ADP/O ratio decreased in the liver mitochondria while state three and four respiration decreased in the brain mitochondria. In both the tissues respiration rates decreased with ascorbate+TMPD as the substrate. In the heart mitochondria ADP/O ratios with NAD+ linked substrates decreased, while respiration rates increased with all the substrates except for ascorbate+TMPD. Temperature kinetics data showed different effects on ATPase in the mitochondria from the three tissues. Data on lipid/phospholipid profiles suggested that the observed changes in energy metabolism were not mediated via lipid changes. Long-term exposure to Al resulted in ≃ 100% increase in Al content of liver and brain mitochondria but in the heart there was phenomenal 11-fold increase, indicating thereby that the effects of Al exposure were indirect rather than direct due to Al accumulation.

Original languageEnglish
Pages (from-to)27-42
Number of pages16
JournalMechanisms of Ageing and Development
Volume112
Issue number1
DOIs
StatePublished - Dec 7 1999
Externally publishedYes

Fingerprint

Heart Mitochondria
Mitochondria
Liver Mitochondrion
Aluminum
Liver
Energy Metabolism
Rats
Brain
Adenosine Diphosphate
Substrates
Respiratory Rate
NAD
Respiration
Tissue
Lipids
Succinic Acid
Adenosine Triphosphatases
Glutamic Acid
Phospholipids
Salts

Keywords

  • Aluminium
  • Alzheimer's disease
  • ATPase
  • Membrane fluidity
  • Mitochondria
  • Oxidative phosphorylation

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Effect of aluminium-induced Alzheimer like condition on oxidative energy metabolism in rat liver, brain and heart mitochondria. / Swegert, Cyril V.; Dave, Kunjan R; Katyare, Surendra S.

In: Mechanisms of Ageing and Development, Vol. 112, No. 1, 07.12.1999, p. 27-42.

Research output: Contribution to journalArticle

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