Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)

Daniela Frasca; Paola Barattini; Donatella Tirindelli; Luisa Guidi; Carlo Bartoloni; Andrea Errani; Mario Costanzo; Augusto Tricerri; Luca Pierelli; Gino Doria

doi:10.1016/S0531-5565(99)00026-1

Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)

Daniela Frasca, Paola Barattini, Donatella Tirindelli, Luisa Guidi, Carlo Bartoloni, Andrea Errani, Mario Costanzo, Augusto Tricerri, Luca Pierelli, Gino Doria

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20-89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which is involved in the earliest steps of DNA damage recognition. DNA binding of ku 70/80 was found unchanged in normal PBMC from aging subjects but progressively declined in x-ray-irradiated PBMC from young to adult, and elderly subjects. This finding was concomitant with the age- related fall of DNA repair in the whole population.

Original language	English (US)
Pages (from-to)	645-658
Number of pages	14
Journal	Experimental Gerontology
Volume	34
Issue number	5
DOIs	https://doi.org/10.1016/S0531-5565(99)00026-1
State	Published - Aug 1999
Externally published	Yes

Keywords

Aging
DNA repair
Ku protein

ASJC Scopus subject areas

Aging
Medicine(all)

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Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC). / Frasca, Daniela; Barattini, Paola; Tirindelli, Donatella; Guidi, Luisa; Bartoloni, Carlo; Errani, Andrea; Costanzo, Mario; Tricerri, Augusto; Pierelli, Luca; Doria, Gino.

In: Experimental Gerontology, Vol. 34, No. 5, 08.1999, p. 645-658.

Research output: Contribution to journal › Article › peer-review

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abstract = "DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20-89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which is involved in the earliest steps of DNA damage recognition. DNA binding of ku 70/80 was found unchanged in normal PBMC from aging subjects but progressively declined in x-ray-irradiated PBMC from young to adult, and elderly subjects. This finding was concomitant with the age- related fall of DNA repair in the whole population.",

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AU - Tirindelli, Donatella

AU - Guidi, Luisa

AU - Bartoloni, Carlo

AU - Errani, Andrea

AU - Costanzo, Mario

AU - Tricerri, Augusto

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AU - Doria, Gino

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