Abstract
DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20-89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which is involved in the earliest steps of DNA damage recognition. DNA binding of ku 70/80 was found unchanged in normal PBMC from aging subjects but progressively declined in x-ray-irradiated PBMC from young to adult, and elderly subjects. This finding was concomitant with the age- related fall of DNA repair in the whole population.
| Original language | English |
|---|---|
| Pages (from-to) | 645-658 |
| Number of pages | 14 |
| Journal | Experimental Gerontology |
| Volume | 34 |
| Issue number | 5 |
| DOIs | |
| State | Published - Aug 1 1999 |
| Externally published | Yes |
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Keywords
- Aging
- DNA repair
- Ku protein
ASJC Scopus subject areas
- Aging
- Medicine(all)
Cite this
Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC). / Frasca, Daniela; Barattini, Paola; Tirindelli, Donatella; Guidi, Luisa; Bartoloni, Carlo; Errani, Andrea; Costanzo, Mario; Tricerri, Augusto; Pierelli, Luca; Doria, Gino.
In: Experimental Gerontology, Vol. 34, No. 5, 01.08.1999, p. 645-658.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)
AU - Frasca, Daniela
AU - Barattini, Paola
AU - Tirindelli, Donatella
AU - Guidi, Luisa
AU - Bartoloni, Carlo
AU - Errani, Andrea
AU - Costanzo, Mario
AU - Tricerri, Augusto
AU - Pierelli, Luca
AU - Doria, Gino
PY - 1999/8/1
Y1 - 1999/8/1
N2 - DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20-89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which is involved in the earliest steps of DNA damage recognition. DNA binding of ku 70/80 was found unchanged in normal PBMC from aging subjects but progressively declined in x-ray-irradiated PBMC from young to adult, and elderly subjects. This finding was concomitant with the age- related fall of DNA repair in the whole population.
AB - DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20-89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which is involved in the earliest steps of DNA damage recognition. DNA binding of ku 70/80 was found unchanged in normal PBMC from aging subjects but progressively declined in x-ray-irradiated PBMC from young to adult, and elderly subjects. This finding was concomitant with the age- related fall of DNA repair in the whole population.
KW - Aging
KW - DNA repair
KW - Ku protein
UR - http://www.scopus.com/inward/record.url?scp=0032828150&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032828150&partnerID=8YFLogxK
U2 - 10.1016/S0531-5565(99)00026-1
DO - 10.1016/S0531-5565(99)00026-1
M3 - Article
VL - 34
SP - 645
EP - 658
JO - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
IS - 5
ER -