Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)

Daniela Frasca, Paola Barattini, Donatella Tirindelli, Luisa Guidi, Carlo Bartoloni, Andrea Errani, Mario Costanzo, Augusto Tricerri, Luca Pierelli, Gino Doria

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20-89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which is involved in the earliest steps of DNA damage recognition. DNA binding of ku 70/80 was found unchanged in normal PBMC from aging subjects but progressively declined in x-ray-irradiated PBMC from young to adult, and elderly subjects. This finding was concomitant with the age- related fall of DNA repair in the whole population.

Original languageEnglish (US)
Pages (from-to)645-658
Number of pages14
JournalExperimental Gerontology
Volume34
Issue number5
DOIs
StatePublished - Aug 1999
Externally publishedYes

Keywords

  • Aging
  • DNA repair
  • Ku protein

ASJC Scopus subject areas

  • Aging
  • Medicine(all)

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    Frasca, D., Barattini, P., Tirindelli, D., Guidi, L., Bartoloni, C., Errani, A., Costanzo, M., Tricerri, A., Pierelli, L., & Doria, G. (1999). Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC). Experimental Gerontology, 34(5), 645-658. https://doi.org/10.1016/S0531-5565(99)00026-1