Effect of adriamycin on the cell cycle traverse and kinetics of cultured human lymphoblasts

A. Krishan, E. Frei

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Exposure of cultured human lymphoblasts to adriamycin (ADM) (0.1 μg/ml for 24 hr or 0.5 μg/ml for 1 hr) leads to an accumulation of cells with the DNA content of late S and G2. Higher concentrations of ADM (0.5 to 10 μg/ml) inhibit cell cycle traverse. Effect of ADM on cell cycle traverse, cell growth, and incorporation of labeled precursors into DNA is dependent on drug concentration and length of exposure to ADM. Synchronized cells in G1 or G2 part of the cell cycle are less sensitive to ADM than cells in S phase. Similarly, plateau phase cells are less sensitive to ADM than cells from log phase cultures.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalCancer Research
Volume36
Issue number1
StatePublished - Dec 1 1976
Externally publishedYes

Fingerprint

Doxorubicin
Cell Cycle
DNA
S Phase
Growth
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effect of adriamycin on the cell cycle traverse and kinetics of cultured human lymphoblasts. / Krishan, A.; Frei, E.

In: Cancer Research, Vol. 36, No. 1, 01.12.1976, p. 143-150.

Research output: Contribution to journalArticle

@article{28d72688c17b4587b62733558c002d27,
title = "Effect of adriamycin on the cell cycle traverse and kinetics of cultured human lymphoblasts",
abstract = "Exposure of cultured human lymphoblasts to adriamycin (ADM) (0.1 μg/ml for 24 hr or 0.5 μg/ml for 1 hr) leads to an accumulation of cells with the DNA content of late S and G2. Higher concentrations of ADM (0.5 to 10 μg/ml) inhibit cell cycle traverse. Effect of ADM on cell cycle traverse, cell growth, and incorporation of labeled precursors into DNA is dependent on drug concentration and length of exposure to ADM. Synchronized cells in G1 or G2 part of the cell cycle are less sensitive to ADM than cells in S phase. Similarly, plateau phase cells are less sensitive to ADM than cells from log phase cultures.",
author = "A. Krishan and E. Frei",
year = "1976",
month = "12",
day = "1",
language = "English",
volume = "36",
pages = "143--150",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "1",

}

TY - JOUR

T1 - Effect of adriamycin on the cell cycle traverse and kinetics of cultured human lymphoblasts

AU - Krishan, A.

AU - Frei, E.

PY - 1976/12/1

Y1 - 1976/12/1

N2 - Exposure of cultured human lymphoblasts to adriamycin (ADM) (0.1 μg/ml for 24 hr or 0.5 μg/ml for 1 hr) leads to an accumulation of cells with the DNA content of late S and G2. Higher concentrations of ADM (0.5 to 10 μg/ml) inhibit cell cycle traverse. Effect of ADM on cell cycle traverse, cell growth, and incorporation of labeled precursors into DNA is dependent on drug concentration and length of exposure to ADM. Synchronized cells in G1 or G2 part of the cell cycle are less sensitive to ADM than cells in S phase. Similarly, plateau phase cells are less sensitive to ADM than cells from log phase cultures.

AB - Exposure of cultured human lymphoblasts to adriamycin (ADM) (0.1 μg/ml for 24 hr or 0.5 μg/ml for 1 hr) leads to an accumulation of cells with the DNA content of late S and G2. Higher concentrations of ADM (0.5 to 10 μg/ml) inhibit cell cycle traverse. Effect of ADM on cell cycle traverse, cell growth, and incorporation of labeled precursors into DNA is dependent on drug concentration and length of exposure to ADM. Synchronized cells in G1 or G2 part of the cell cycle are less sensitive to ADM than cells in S phase. Similarly, plateau phase cells are less sensitive to ADM than cells from log phase cultures.

UR - http://www.scopus.com/inward/record.url?scp=0017284035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017284035&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 143

EP - 150

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 1

ER -