Abstract
Hypertrophied hearts have enhanced susceptibility to ischemia-induced contractile dysfunction. To explore the mechanisms of this phenomenon, we studied the effect of acidosis on the Ca2+ sensitivity of the contractile proteins and on Ca2+ accumulation by the sarcoplasmic reticulum (SR) in chemically (saponin) skinned cardiac fibers obtained from normal and pressure-overload hypertrophied rat left ventricles. Left ventricular pressure overload was induced by partial ligation of the abdominal aorta 6-8 wk before study. Age- and weight-matched normal rats served as controls. Pressure overload increased the left ventricular weight-to-body weight ratio by 48%. Reduction in pH shifted the pCa-tension curve to the right similarly in normal and hypertrophied preparations, and there was no difference in pCa-tension relationship at pH 7.0 or 6.5 between the two groups. However, reducing the pH of 1 μM Ca2+-loading solution from 7.0 to 6.5 decreased the amount of Ca2+ accumulated in the SR to 66.2 ± 3.0% in normal fibers and 43.2 ± 4.0% in hypertrophied fibers (P < 0.01). We conclude that the enhanced susceptibility of hypertrophied hearts to ischemia-induced diastolic dysfunction may be partly explained by the greater depressing effect of acidosis on Ca2+ accumulation by the SR.
Original language | English (US) |
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Pages (from-to) | H1044-H1049 |
Journal | American Journal of Physiology - Heart and Circulatory Physiology |
Volume | 259 |
Issue number | 4 28-4 |
DOIs | |
State | Published - 1990 |
Keywords
- aortic constriction
- calcium sensitivity
- chronic pressure overload
- sarcoplasmic reticulum
ASJC Scopus subject areas
- Physiology