Edema and vascular permeability in cerebral ischemia

Comparison between ischemic neuronal damage and infarction

Carol Petito, W. A. Pulsinelli, G. Jacobson, F. Plum

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

The respective influences of ischemic neuronal damage and infarction on the development of abnormal blood-brain barrier (BBB) permeability and cerebral edema were evaluated in a rat model of temporary four-vessel occlusion in which ischemic neuronal damage with only infrequent infarction is produced. Survival times ranged from 40 minutes to 5 days after ischemia. Evans blue and horseradish peroxidase (HRP) were given before sacrifice. The majority of brains showed moderate ischemic neuronal damage in the striatum. In these areas there was neither leakage of Evans blue nor extravasation of HRP. Astrocytic processes were moderately swollen. Large, grossly-visible unilateral infarcts were present in only 5 animals, and all showed abnormal BBB permeability of HRP which occurred via enhanced pinocytosis, and occasionally via diffuse leakage through necrotic vessels. Astrocytic processes were markedly swollen and their plasma membranes were disrupted. Whole brain and regional water content in parallel series of animals were measured from 15 minutes (min) to 48 hours (hr) postischemia. They showed a transient, 1% increase in whole brain water content from 15 to 60 min postischemia, but no increase in regional water content at any postischemic interval. These studies suggest that ischemia produces BBB permeability to large molecules, and sustained cerebral edema only when the process damages blood vessels and astrocytes; neuronal necrosis alone is insufficient.

Original languageEnglish
Pages (from-to)423-436
Number of pages14
JournalJournal of Neuropathology and Experimental Neurology
Volume41
Issue number4
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

Capillary Permeability
Horseradish Peroxidase
Brain Ischemia
Blood-Brain Barrier
Infarction
Permeability
Edema
Evans Blue
Brain Edema
Water
Brain
Ischemia
Pinocytosis
Astrocytes
Blood Vessels
Necrosis
Cell Membrane

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Edema and vascular permeability in cerebral ischemia : Comparison between ischemic neuronal damage and infarction. / Petito, Carol; Pulsinelli, W. A.; Jacobson, G.; Plum, F.

In: Journal of Neuropathology and Experimental Neurology, Vol. 41, No. 4, 01.01.1982, p. 423-436.

Research output: Contribution to journalArticle

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