Ectopic protein interactions within BRD4-chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma

Artyom A. Alekseyenko, Erica M. Walsh, Barry M. Zee, Tibor Pakozdi, Peter Hsi, Madeleine E. Lemieux, Paola Dal Cin, Tan Ince, Peter V. Kharchenko, Mitzi I. Kuroda, Christopher A. French

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To investigate the mechanism that drives dramatic mistargeting of active chromatin in NUT midline carcinoma (NMC), we have identified protein interactions unique to the BRD4-NUT fusion oncoprotein compared with wild-type BRD4. Using cross-linking, affinity purification, and mass spectrometry, we identified the EP300 acetyltransferase as uniquely associated with BRD4 through the NUT fusion in both NMC and non-NMC cell types. We also discovered ZNF532 associated with BRD4-NUT in NMC patient cells but not detectable in 293T cells. EP300 and ZNF532 are both implicated in feed-forward regulatory loops leading to propagation of the oncogenic chromatin complex in BRD4-NUT patient cells. Adding key functional significance to our biochemical findings, we independently discovered a ZNF532-NUT translocation fusion in a newly diagnosed NMC patient. ChIP sequencing of the major players NUT, ZNF532, BRD4, EP300, and H3K27ac revealed the formation of ZNF532-NUT-associated hyperacetylated megadomains, distinctly localized but otherwise analogous to those found in BRD4- NUT patient cells. Our results support a model in which NMC is dependent on ectopic NUT-mediated interactions between EP300 and components of BRD4 regulatory complexes, leading to a cascade of misregulation.

Original languageEnglish (US)
Pages (from-to)E4184-E4192
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number21
DOIs
StatePublished - May 23 2017

Fingerprint

Chromatin
Carcinoma
Proteins
Acetyltransferases
HEK293 Cells
Oncogene Proteins
Mass Spectrometry

Keywords

  • BioTAP-XL
  • BRD4
  • Hyperacetylation
  • Topological domains
  • ZNF532-NUT

ASJC Scopus subject areas

  • General

Cite this

Ectopic protein interactions within BRD4-chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma. / Alekseyenko, Artyom A.; Walsh, Erica M.; Zee, Barry M.; Pakozdi, Tibor; Hsi, Peter; Lemieux, Madeleine E.; Dal Cin, Paola; Ince, Tan; Kharchenko, Peter V.; Kuroda, Mitzi I.; French, Christopher A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 21, 23.05.2017, p. E4184-E4192.

Research output: Contribution to journalArticle

Alekseyenko, AA, Walsh, EM, Zee, BM, Pakozdi, T, Hsi, P, Lemieux, ME, Dal Cin, P, Ince, T, Kharchenko, PV, Kuroda, MI & French, CA 2017, 'Ectopic protein interactions within BRD4-chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma', Proceedings of the National Academy of Sciences of the United States of America, vol. 114, no. 21, pp. E4184-E4192. https://doi.org/10.1073/pnas.1702086114
Alekseyenko, Artyom A. ; Walsh, Erica M. ; Zee, Barry M. ; Pakozdi, Tibor ; Hsi, Peter ; Lemieux, Madeleine E. ; Dal Cin, Paola ; Ince, Tan ; Kharchenko, Peter V. ; Kuroda, Mitzi I. ; French, Christopher A. / Ectopic protein interactions within BRD4-chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 21. pp. E4184-E4192.
@article{9eecbf34ddd04c1884db2a563feb17e3,
title = "Ectopic protein interactions within BRD4-chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma",
abstract = "To investigate the mechanism that drives dramatic mistargeting of active chromatin in NUT midline carcinoma (NMC), we have identified protein interactions unique to the BRD4-NUT fusion oncoprotein compared with wild-type BRD4. Using cross-linking, affinity purification, and mass spectrometry, we identified the EP300 acetyltransferase as uniquely associated with BRD4 through the NUT fusion in both NMC and non-NMC cell types. We also discovered ZNF532 associated with BRD4-NUT in NMC patient cells but not detectable in 293T cells. EP300 and ZNF532 are both implicated in feed-forward regulatory loops leading to propagation of the oncogenic chromatin complex in BRD4-NUT patient cells. Adding key functional significance to our biochemical findings, we independently discovered a ZNF532-NUT translocation fusion in a newly diagnosed NMC patient. ChIP sequencing of the major players NUT, ZNF532, BRD4, EP300, and H3K27ac revealed the formation of ZNF532-NUT-associated hyperacetylated megadomains, distinctly localized but otherwise analogous to those found in BRD4- NUT patient cells. Our results support a model in which NMC is dependent on ectopic NUT-mediated interactions between EP300 and components of BRD4 regulatory complexes, leading to a cascade of misregulation.",
keywords = "BioTAP-XL, BRD4, Hyperacetylation, Topological domains, ZNF532-NUT",
author = "Alekseyenko, {Artyom A.} and Walsh, {Erica M.} and Zee, {Barry M.} and Tibor Pakozdi and Peter Hsi and Lemieux, {Madeleine E.} and {Dal Cin}, Paola and Tan Ince and Kharchenko, {Peter V.} and Kuroda, {Mitzi I.} and French, {Christopher A.}",
year = "2017",
month = "5",
day = "23",
doi = "10.1073/pnas.1702086114",
language = "English (US)",
volume = "114",
pages = "E4184--E4192",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "21",

}

TY - JOUR

T1 - Ectopic protein interactions within BRD4-chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma

AU - Alekseyenko, Artyom A.

AU - Walsh, Erica M.

AU - Zee, Barry M.

AU - Pakozdi, Tibor

AU - Hsi, Peter

AU - Lemieux, Madeleine E.

AU - Dal Cin, Paola

AU - Ince, Tan

AU - Kharchenko, Peter V.

AU - Kuroda, Mitzi I.

AU - French, Christopher A.

PY - 2017/5/23

Y1 - 2017/5/23

N2 - To investigate the mechanism that drives dramatic mistargeting of active chromatin in NUT midline carcinoma (NMC), we have identified protein interactions unique to the BRD4-NUT fusion oncoprotein compared with wild-type BRD4. Using cross-linking, affinity purification, and mass spectrometry, we identified the EP300 acetyltransferase as uniquely associated with BRD4 through the NUT fusion in both NMC and non-NMC cell types. We also discovered ZNF532 associated with BRD4-NUT in NMC patient cells but not detectable in 293T cells. EP300 and ZNF532 are both implicated in feed-forward regulatory loops leading to propagation of the oncogenic chromatin complex in BRD4-NUT patient cells. Adding key functional significance to our biochemical findings, we independently discovered a ZNF532-NUT translocation fusion in a newly diagnosed NMC patient. ChIP sequencing of the major players NUT, ZNF532, BRD4, EP300, and H3K27ac revealed the formation of ZNF532-NUT-associated hyperacetylated megadomains, distinctly localized but otherwise analogous to those found in BRD4- NUT patient cells. Our results support a model in which NMC is dependent on ectopic NUT-mediated interactions between EP300 and components of BRD4 regulatory complexes, leading to a cascade of misregulation.

AB - To investigate the mechanism that drives dramatic mistargeting of active chromatin in NUT midline carcinoma (NMC), we have identified protein interactions unique to the BRD4-NUT fusion oncoprotein compared with wild-type BRD4. Using cross-linking, affinity purification, and mass spectrometry, we identified the EP300 acetyltransferase as uniquely associated with BRD4 through the NUT fusion in both NMC and non-NMC cell types. We also discovered ZNF532 associated with BRD4-NUT in NMC patient cells but not detectable in 293T cells. EP300 and ZNF532 are both implicated in feed-forward regulatory loops leading to propagation of the oncogenic chromatin complex in BRD4-NUT patient cells. Adding key functional significance to our biochemical findings, we independently discovered a ZNF532-NUT translocation fusion in a newly diagnosed NMC patient. ChIP sequencing of the major players NUT, ZNF532, BRD4, EP300, and H3K27ac revealed the formation of ZNF532-NUT-associated hyperacetylated megadomains, distinctly localized but otherwise analogous to those found in BRD4- NUT patient cells. Our results support a model in which NMC is dependent on ectopic NUT-mediated interactions between EP300 and components of BRD4 regulatory complexes, leading to a cascade of misregulation.

KW - BioTAP-XL

KW - BRD4

KW - Hyperacetylation

KW - Topological domains

KW - ZNF532-NUT

UR - http://www.scopus.com/inward/record.url?scp=85019557187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019557187&partnerID=8YFLogxK

U2 - 10.1073/pnas.1702086114

DO - 10.1073/pnas.1702086114

M3 - Article

VL - 114

SP - E4184-E4192

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 21

ER -