Ectopic ossification in the scar tissue of rats with myocardial infarction

Karla Consort Ribeiro, Elisabete César Mattos, João Pedro Saar Werneck-De-Castro, Vanessa Pinho Ribeiro, Ricardo Henrique Costa-E-Sousa, Amarildo Miranda, Emerson Lopes Olivares, Marcos Farina, José Geraldo Mill, Regina Coeli Dos Santos Goldenberg, Masako Oya Masuda, Antônio Carlos Campos De Carvalho

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We describe the occurrence of bone-like formations in the left ventricular wall of infarcted rats treated or not with bone marrow cells injected systemically or locally into the myocardium. The incidence of ectopic calcification in hearts has been reported in rare cases in children with infarcts without previous coronary artery disease. Recently, ventricular calcification has been correlated with unselected bone marrow cell transplantation into infarcted rat hearts. Echocardiographic analysis of large infarction in rats frequently reveals the presence of echogenic structures in the left ventricular wall, sometimes projecting to the lumen of the chamber. The histological examination of these echogenic structures exhibited bone, cartilage, and marrow-like formations extending from the collagen-rich matrix of the ventricle wall. Microanalytical techniques verified the presence of hydroxyapatite in the mineral phase. Ossification was found in 25 out of 30 hearts evaluated 90 days postinfarct, being observed in 14 out of 17 animals submitted to cell therapy and in 11 out of 13 infarcted rats not submitted to cell therapy. Our study indicates that chondro-osteogenic differentiation can take place in the pathological rat heart independent of animal treatment with marrow cells.

Original languageEnglish (US)
Pages (from-to)389-397
Number of pages9
JournalCell transplantation
Volume15
Issue number5
DOIs
StatePublished - 2006
Externally publishedYes

Keywords

  • Correlative microscopy
  • Ectopic ossification
  • Myocardial infarction
  • Osteogenesis

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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