Ectopic expression of GATA6 bypasses requirement for Grb2 in primitive endoderm formation

Yingcai Wang, Jennifer L. Smedberg, Kathy Qi Cai, D. Callinice Capo-Chichi, Xiangxi Xu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Gene knockouts in mice have showed that Grb2 and GATA6 are essential for the formation of primitive endoderm in blastocysts. Here, we confirmed that implanted Grb2-null blastocysts lack primitive or extraembryonic endoderm cells either at E4.5 or E5.5 stages. We analyzed the relationship between Grb2 and GATA6 in the differentiation of embryonic stem (ES) cells to primitive endoderm in embryoid body models. Upon transfection with GATA6 expression vector, Grb2-null ES cells underwent endoderm differentiation as indicated by the expression of the extraembryonic endoderm markers Dab2 and GATA4. Transfection of GATA4 expression vector also had the same differentiation potency. When GATA6- or GATA4-transfected Grb2-null ES cells were allowed to aggregate, fragments of an endoderm layer formed on the surface of the spheroids. The results suggest that GATA6 is downstream of Grb2 in the inductive signaling pathway and the expression of GATA6 is sufficient to compensate for the defects caused by Grb2 deficiency in the development of the primitive and extraembryonic endoderm. Developmental Dynamics 240:566-576, 2011.

Original languageEnglish
Pages (from-to)566-576
Number of pages11
JournalDevelopmental Dynamics
Volume240
Issue number3
DOIs
StatePublished - Mar 1 2011

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Endoderm
Embryonic Stem Cells
Blastocyst
Transfection
Embryoid Bodies
Gene Knockout Techniques
Ectopic Gene Expression
Knockout Mice

Keywords

  • Blastocysts
  • Embryoid bodies
  • ES cells
  • Lineage differentiation
  • Primitive endoderm
  • Ras/MAPK pathway

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Ectopic expression of GATA6 bypasses requirement for Grb2 in primitive endoderm formation. / Wang, Yingcai; Smedberg, Jennifer L.; Cai, Kathy Qi; Capo-Chichi, D. Callinice; Xu, Xiangxi.

In: Developmental Dynamics, Vol. 240, No. 3, 01.03.2011, p. 566-576.

Research output: Contribution to journalArticle

Wang, Yingcai ; Smedberg, Jennifer L. ; Cai, Kathy Qi ; Capo-Chichi, D. Callinice ; Xu, Xiangxi. / Ectopic expression of GATA6 bypasses requirement for Grb2 in primitive endoderm formation. In: Developmental Dynamics. 2011 ; Vol. 240, No. 3. pp. 566-576.
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