TY - JOUR
T1 - EBV microRNAs in primary lymphomas and targeting of CXCL-11 by ebv-mir-BHRF1-3
AU - Xia, Tianli
AU - O'Hara, Andrea
AU - Araujo, Iguaracyra
AU - Barreto, Jose
AU - Carvalho, Eny
AU - Sapucaia, Jose Bahia
AU - Ramos, Juan Carlos
AU - Luz, Estela
AU - Pedroso, Celia
AU - Manrique, Michele
AU - Toomey, Ngoc L.
AU - Brites, Carlos
AU - Dittmer, Dirk P.
AU - Harrington, William J.
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/3/1
Y1 - 2008/3/1
N2 - EBV-encoded microRNAs (miRNAs) have been identified and their functions are being studied. The expression pattern of these miRNAs in clinical samples of EBV-associated non-Hodgkin's lymphomas is unknown. We analyzed five primary "endemic" pediatric Burkitt's lymphomas (BL), two acquired immunodeficiency syndrome (AIDS)-related type I latency BL lines, a type III latency line, three EBV+ primary effusion lymphomas (PEL), and three AIDS-related diffuse large B-cell lymphomas (DLBCL) for expression of EBV-encoded miRNAs. A markedly elevated expression of miRNA BHRF1-3 in type III relative to its parental type I BL line was found. Primary unmanipulated type I BLs and EBV+ PELs expressed high levels of BART2 miRNA, whereas DLBCLs expressed both BART2 and BHRF1-3 species. BHRF1-3 miRNA expression inversely correlated with levels of a putative cellular target, the IFN-inducible T-cell attracting chemokine CXCL-11/I-TAC, and suppression of this factor was reversed by transfection of an antisense oligo to the EBV miRNA BHRF1-3. EBV-encoded miRNAs are expressed in primary lymphomas classically linked to the virus and are associated with the viral latency status. Targeted suppression of CXCL-11/I-TAC by a viral-encoded miRNA may serve as an immunomodulatory mechanism in these tumors.
AB - EBV-encoded microRNAs (miRNAs) have been identified and their functions are being studied. The expression pattern of these miRNAs in clinical samples of EBV-associated non-Hodgkin's lymphomas is unknown. We analyzed five primary "endemic" pediatric Burkitt's lymphomas (BL), two acquired immunodeficiency syndrome (AIDS)-related type I latency BL lines, a type III latency line, three EBV+ primary effusion lymphomas (PEL), and three AIDS-related diffuse large B-cell lymphomas (DLBCL) for expression of EBV-encoded miRNAs. A markedly elevated expression of miRNA BHRF1-3 in type III relative to its parental type I BL line was found. Primary unmanipulated type I BLs and EBV+ PELs expressed high levels of BART2 miRNA, whereas DLBCLs expressed both BART2 and BHRF1-3 species. BHRF1-3 miRNA expression inversely correlated with levels of a putative cellular target, the IFN-inducible T-cell attracting chemokine CXCL-11/I-TAC, and suppression of this factor was reversed by transfection of an antisense oligo to the EBV miRNA BHRF1-3. EBV-encoded miRNAs are expressed in primary lymphomas classically linked to the virus and are associated with the viral latency status. Targeted suppression of CXCL-11/I-TAC by a viral-encoded miRNA may serve as an immunomodulatory mechanism in these tumors.
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U2 - 10.1158/0008-5472.CAN-07-5126
DO - 10.1158/0008-5472.CAN-07-5126
M3 - Article
C2 - 18316607
AN - SCOPUS:40449141224
VL - 68
SP - 1436
EP - 1442
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 5
ER -