EBV microRNAs in primary lymphomas and targeting of CXCL-11 by ebv-mir-BHRF1-3

Tianli Xia, Andrea O'Hara, Iguaracyra Araujo, Jose Barreto, Eny Carvalho, Jose Bahia Sapucaia, Juan Carlos Ramos, Estela Luz, Celia Pedroso, Michele Manrique, Ngoc L. Toomey, Carlos Brites, Dirk P. Dittmer, William J. Harrington

Research output: Contribution to journalArticlepeer-review

248 Scopus citations


EBV-encoded microRNAs (miRNAs) have been identified and their functions are being studied. The expression pattern of these miRNAs in clinical samples of EBV-associated non-Hodgkin's lymphomas is unknown. We analyzed five primary "endemic" pediatric Burkitt's lymphomas (BL), two acquired immunodeficiency syndrome (AIDS)-related type I latency BL lines, a type III latency line, three EBV+ primary effusion lymphomas (PEL), and three AIDS-related diffuse large B-cell lymphomas (DLBCL) for expression of EBV-encoded miRNAs. A markedly elevated expression of miRNA BHRF1-3 in type III relative to its parental type I BL line was found. Primary unmanipulated type I BLs and EBV+ PELs expressed high levels of BART2 miRNA, whereas DLBCLs expressed both BART2 and BHRF1-3 species. BHRF1-3 miRNA expression inversely correlated with levels of a putative cellular target, the IFN-inducible T-cell attracting chemokine CXCL-11/I-TAC, and suppression of this factor was reversed by transfection of an antisense oligo to the EBV miRNA BHRF1-3. EBV-encoded miRNAs are expressed in primary lymphomas classically linked to the virus and are associated with the viral latency status. Targeted suppression of CXCL-11/I-TAC by a viral-encoded miRNA may serve as an immunomodulatory mechanism in these tumors.

Original languageEnglish (US)
Pages (from-to)1436-1442
Number of pages7
JournalCancer Research
Issue number5
StatePublished - Mar 1 2008

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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