Easy-To-Perform Dual Immunohistochemistry Can Solve Problems in Hematopathology

Julio Poveda, Daniel P. Cassidy, Yi Zhou, Jennifer Chapman

Research output: Contribution to journalArticlepeer-review


Immunohistochemical analysis has become an integral component in the diagnostic work up of hematopoietic neoplasms. It is not uncommon that visualization of single protein expression by immunohistochemistry among cells of interest may become a difficult task. Common scenarios of such include extensive colonization of germinal centers in the differential diagnosis of marginal zone lymphoma and follicular lymphoma, low-level bone marrow involvement by lymphoma and paucity of neoplastic lymphocytes in the setting of numerous background reactive lymphocytes, among others. For this reason, we have developed a variety of easy-to-employ dual-color dual-antibody immunohistochemical assays to aid in solving these diagnostic dilemmas. Herein, we share examples of our use of dual immunohistochemistry to illustrate its beneficial and practical objective.

Original languageEnglish (US)
Pages (from-to)225-235
Number of pages11
JournalApplied Immunohistochemistry and Molecular Morphology
Issue number3
StatePublished - Mar 1 2022
Externally publishedYes


  • hematopathology
  • immunohistochemistry
  • immunophenotyping
  • lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Medical Laboratory Technology


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