Early transcriptional responses during heat stress in the coral Acropora hyacinthus

Nikki Traylor-Knowles, Noah H. Rose, Elizabeth A. Sheets, Stephen R. Palumbi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Corals respond to heat pulses that cause bleaching with massive transcriptional change, but the immediate responses to stress that lead up to these shifts have never been detailed. Understanding these early signals could be important for identifying the regulatory mechanisms responsible for bleaching and how these mechanisms vary between more and less resilient corals. Using RNA sequencing (RNAseq) and sampling every 30 minutes during a short-term heat shock, we found that components of the transcriptome were significantly upregulated within 90 min and after a temperature increase of +2 °C. The developmental transcription factor, Krüppel-like factor 7, was highly expressed within 60 min, and stress-related transcription factors such as Elk-3 were highly expressed starting at 240 min. The sets of genes enriched for early transcriptional response to heat stress included heat shock proteins, small GTPases, and proteasome genes. Retrovirus-related Pol polyproteins from transposons were significantly expressed throughout the whole experiment. Lastly, we propose a model for early transcriptional regulation of protein degradation and cell adhesion response that may ultimately lead to the bleaching and stress response.

Original languageEnglish (US)
Pages (from-to)91-100
Number of pages10
JournalBiological Bulletin
Volume232
Issue number2
DOIs
StatePublished - Jan 1 2017

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Hyacinthus
Heat-Shock Response
Anthozoa
Acropora
bleaching
corals
Transcription Factors
pol Gene Products
Hot Temperature
RNA Sequence Analysis
heat stress
Monomeric GTP-Binding Proteins
stress response
Retroviridae
Proteasome Endopeptidase Complex
transcription factors
Heat-Shock Proteins
Transcriptome
Cell Adhesion
Proteolysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Early transcriptional responses during heat stress in the coral Acropora hyacinthus. / Traylor-Knowles, Nikki; Rose, Noah H.; Sheets, Elizabeth A.; Palumbi, Stephen R.

In: Biological Bulletin, Vol. 232, No. 2, 01.01.2017, p. 91-100.

Research output: Contribution to journalArticle

Traylor-Knowles, Nikki ; Rose, Noah H. ; Sheets, Elizabeth A. ; Palumbi, Stephen R. / Early transcriptional responses during heat stress in the coral Acropora hyacinthus. In: Biological Bulletin. 2017 ; Vol. 232, No. 2. pp. 91-100.
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