Early pregnancy decidual lymphocytes beside perforin use Fas ligand (FasL) mediated cytotoxicity

Tatjana Bogovic Crncic, Gordana Laskarin, Koraljka Juretic Frankovic, Vlatka Sotosek Tokmadzic, Natasa Strbo, Ivica Bedenicki, Philippe Le Bouteiller, Julie Tabiasco, Daniel Rukavina

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Decidual natural killer (NK) cells are the predominant lymphocytes at the maternal-fetal interface. They are involved in defense against virally infected, parasitized and transformed cells and may contribute to the control of trophoblast invasion. The presence of perforin and other possible cytolytic mediators suggests these functions. Cytolytic mechanisms of unstimulated and Th1 cytokine stimulated decidual lymphocytes (DL), as well as purified decidual CD56+ cells, were analyzed against NK sensitive and resistant targets. DL were isolated from decidual mononuclear cells (DMC) cultured in the medium only or in the presence of Th1 cytokines: IL-2, IL-12, IL-15, IL-18 and their combinations (IL-12/IL-18 or IL-15/IL-18). Fas ligand (FasL), perforin and granzyme B mRNAs expression and cytotoxicity were analyzed by flow cytometry and/or RT-PCR. DL (containing 72.19 ± 7.53% of CD56+ cells), obtained from 18 h-cultured DMC in the medium only, expressed perforin, FasL and granzyme B mRNAs and lysed the NK-sensitive K-562 cell line, and also the NK-resistant P815 and P815-Fas transfected cell lines. Concanamycin A, a blocker of granule exocytosis, decreased significantly K-562 lysis, but not P815 lysis. However, the addition of anti-FasL antibody diminished significantly P815 lysis as well. IL-2 and IL-15, known inducers of perforin and FasL mRNAs and protein expression, could not additionally increase P 815 cell lysis by DL cultured within DMC. These results suggest that DL cultured in DMC for 18 h, have the characteristics of lymphokine-activated killer (LAK) cells and are able to use efficiently both the perforin and the FasL cytolytic pathways.

Original languageEnglish (US)
Pages (from-to)108-117
Number of pages10
JournalJournal of Reproductive Immunology
Volume73
Issue number2
DOIs
StatePublished - Apr 2007
Externally publishedYes

Fingerprint

Perforin
Fas Ligand Protein
Lymphocytes
Pregnancy
Interleukin-15
Interleukin-18
Interleukin-12
Messenger RNA
Interleukin-2
Cytokines
Lymphokine-Activated Killer Cells
Granzymes
Cell Line
Exocytosis
Trophoblasts
Natural Killer Cells
Cultured Cells
Flow Cytometry
Mothers
Polymerase Chain Reaction

Keywords

  • Cytotoxicity
  • Decidual lymphocytes
  • Fas ligand
  • Perforin
  • Th1 cytokines

ASJC Scopus subject areas

  • Immunology
  • Reproductive Medicine

Cite this

Crncic, T. B., Laskarin, G., Frankovic, K. J., Tokmadzic, V. S., Strbo, N., Bedenicki, I., ... Rukavina, D. (2007). Early pregnancy decidual lymphocytes beside perforin use Fas ligand (FasL) mediated cytotoxicity. Journal of Reproductive Immunology, 73(2), 108-117. https://doi.org/10.1016/j.jri.2006.07.001

Early pregnancy decidual lymphocytes beside perforin use Fas ligand (FasL) mediated cytotoxicity. / Crncic, Tatjana Bogovic; Laskarin, Gordana; Frankovic, Koraljka Juretic; Tokmadzic, Vlatka Sotosek; Strbo, Natasa; Bedenicki, Ivica; Le Bouteiller, Philippe; Tabiasco, Julie; Rukavina, Daniel.

In: Journal of Reproductive Immunology, Vol. 73, No. 2, 04.2007, p. 108-117.

Research output: Contribution to journalArticle

Crncic, TB, Laskarin, G, Frankovic, KJ, Tokmadzic, VS, Strbo, N, Bedenicki, I, Le Bouteiller, P, Tabiasco, J & Rukavina, D 2007, 'Early pregnancy decidual lymphocytes beside perforin use Fas ligand (FasL) mediated cytotoxicity', Journal of Reproductive Immunology, vol. 73, no. 2, pp. 108-117. https://doi.org/10.1016/j.jri.2006.07.001
Crncic, Tatjana Bogovic ; Laskarin, Gordana ; Frankovic, Koraljka Juretic ; Tokmadzic, Vlatka Sotosek ; Strbo, Natasa ; Bedenicki, Ivica ; Le Bouteiller, Philippe ; Tabiasco, Julie ; Rukavina, Daniel. / Early pregnancy decidual lymphocytes beside perforin use Fas ligand (FasL) mediated cytotoxicity. In: Journal of Reproductive Immunology. 2007 ; Vol. 73, No. 2. pp. 108-117.
@article{9164c87424ad4ab68eb1de95db4d3b17,
title = "Early pregnancy decidual lymphocytes beside perforin use Fas ligand (FasL) mediated cytotoxicity",
abstract = "Decidual natural killer (NK) cells are the predominant lymphocytes at the maternal-fetal interface. They are involved in defense against virally infected, parasitized and transformed cells and may contribute to the control of trophoblast invasion. The presence of perforin and other possible cytolytic mediators suggests these functions. Cytolytic mechanisms of unstimulated and Th1 cytokine stimulated decidual lymphocytes (DL), as well as purified decidual CD56+ cells, were analyzed against NK sensitive and resistant targets. DL were isolated from decidual mononuclear cells (DMC) cultured in the medium only or in the presence of Th1 cytokines: IL-2, IL-12, IL-15, IL-18 and their combinations (IL-12/IL-18 or IL-15/IL-18). Fas ligand (FasL), perforin and granzyme B mRNAs expression and cytotoxicity were analyzed by flow cytometry and/or RT-PCR. DL (containing 72.19 ± 7.53{\%} of CD56+ cells), obtained from 18 h-cultured DMC in the medium only, expressed perforin, FasL and granzyme B mRNAs and lysed the NK-sensitive K-562 cell line, and also the NK-resistant P815 and P815-Fas transfected cell lines. Concanamycin A, a blocker of granule exocytosis, decreased significantly K-562 lysis, but not P815 lysis. However, the addition of anti-FasL antibody diminished significantly P815 lysis as well. IL-2 and IL-15, known inducers of perforin and FasL mRNAs and protein expression, could not additionally increase P 815 cell lysis by DL cultured within DMC. These results suggest that DL cultured in DMC for 18 h, have the characteristics of lymphokine-activated killer (LAK) cells and are able to use efficiently both the perforin and the FasL cytolytic pathways.",
keywords = "Cytotoxicity, Decidual lymphocytes, Fas ligand, Perforin, Th1 cytokines",
author = "Crncic, {Tatjana Bogovic} and Gordana Laskarin and Frankovic, {Koraljka Juretic} and Tokmadzic, {Vlatka Sotosek} and Natasa Strbo and Ivica Bedenicki and {Le Bouteiller}, Philippe and Julie Tabiasco and Daniel Rukavina",
year = "2007",
month = "4",
doi = "10.1016/j.jri.2006.07.001",
language = "English (US)",
volume = "73",
pages = "108--117",
journal = "Journal of Reproductive Immunology",
issn = "0165-0378",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Early pregnancy decidual lymphocytes beside perforin use Fas ligand (FasL) mediated cytotoxicity

AU - Crncic, Tatjana Bogovic

AU - Laskarin, Gordana

AU - Frankovic, Koraljka Juretic

AU - Tokmadzic, Vlatka Sotosek

AU - Strbo, Natasa

AU - Bedenicki, Ivica

AU - Le Bouteiller, Philippe

AU - Tabiasco, Julie

AU - Rukavina, Daniel

PY - 2007/4

Y1 - 2007/4

N2 - Decidual natural killer (NK) cells are the predominant lymphocytes at the maternal-fetal interface. They are involved in defense against virally infected, parasitized and transformed cells and may contribute to the control of trophoblast invasion. The presence of perforin and other possible cytolytic mediators suggests these functions. Cytolytic mechanisms of unstimulated and Th1 cytokine stimulated decidual lymphocytes (DL), as well as purified decidual CD56+ cells, were analyzed against NK sensitive and resistant targets. DL were isolated from decidual mononuclear cells (DMC) cultured in the medium only or in the presence of Th1 cytokines: IL-2, IL-12, IL-15, IL-18 and their combinations (IL-12/IL-18 or IL-15/IL-18). Fas ligand (FasL), perforin and granzyme B mRNAs expression and cytotoxicity were analyzed by flow cytometry and/or RT-PCR. DL (containing 72.19 ± 7.53% of CD56+ cells), obtained from 18 h-cultured DMC in the medium only, expressed perforin, FasL and granzyme B mRNAs and lysed the NK-sensitive K-562 cell line, and also the NK-resistant P815 and P815-Fas transfected cell lines. Concanamycin A, a blocker of granule exocytosis, decreased significantly K-562 lysis, but not P815 lysis. However, the addition of anti-FasL antibody diminished significantly P815 lysis as well. IL-2 and IL-15, known inducers of perforin and FasL mRNAs and protein expression, could not additionally increase P 815 cell lysis by DL cultured within DMC. These results suggest that DL cultured in DMC for 18 h, have the characteristics of lymphokine-activated killer (LAK) cells and are able to use efficiently both the perforin and the FasL cytolytic pathways.

AB - Decidual natural killer (NK) cells are the predominant lymphocytes at the maternal-fetal interface. They are involved in defense against virally infected, parasitized and transformed cells and may contribute to the control of trophoblast invasion. The presence of perforin and other possible cytolytic mediators suggests these functions. Cytolytic mechanisms of unstimulated and Th1 cytokine stimulated decidual lymphocytes (DL), as well as purified decidual CD56+ cells, were analyzed against NK sensitive and resistant targets. DL were isolated from decidual mononuclear cells (DMC) cultured in the medium only or in the presence of Th1 cytokines: IL-2, IL-12, IL-15, IL-18 and their combinations (IL-12/IL-18 or IL-15/IL-18). Fas ligand (FasL), perforin and granzyme B mRNAs expression and cytotoxicity were analyzed by flow cytometry and/or RT-PCR. DL (containing 72.19 ± 7.53% of CD56+ cells), obtained from 18 h-cultured DMC in the medium only, expressed perforin, FasL and granzyme B mRNAs and lysed the NK-sensitive K-562 cell line, and also the NK-resistant P815 and P815-Fas transfected cell lines. Concanamycin A, a blocker of granule exocytosis, decreased significantly K-562 lysis, but not P815 lysis. However, the addition of anti-FasL antibody diminished significantly P815 lysis as well. IL-2 and IL-15, known inducers of perforin and FasL mRNAs and protein expression, could not additionally increase P 815 cell lysis by DL cultured within DMC. These results suggest that DL cultured in DMC for 18 h, have the characteristics of lymphokine-activated killer (LAK) cells and are able to use efficiently both the perforin and the FasL cytolytic pathways.

KW - Cytotoxicity

KW - Decidual lymphocytes

KW - Fas ligand

KW - Perforin

KW - Th1 cytokines

UR - http://www.scopus.com/inward/record.url?scp=33947105435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947105435&partnerID=8YFLogxK

U2 - 10.1016/j.jri.2006.07.001

DO - 10.1016/j.jri.2006.07.001

M3 - Article

C2 - 16950518

AN - SCOPUS:33947105435

VL - 73

SP - 108

EP - 117

JO - Journal of Reproductive Immunology

JF - Journal of Reproductive Immunology

SN - 0165-0378

IS - 2

ER -