Early passage bone marrow stromal cells express genes involved in nervous system development supporting their relevance for neural repair

R. D S Nandoe Tewarie, K. Bossers, G. J. Ritfeld, B. Blits, J. A. Grotenhuis, J. Verhaagen, Martin Oudega

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: The assessment of the capacity of bone marrow stromal cells (BMSC) to repair the nervous system using gene expression profiling. The evaluation of effects of long-term culturing on the gene expression profile of BMSC. Methods: Fourty four k whole genome rat microarrays were used to study gene expression of cultured BMSC at passage (P)3 and to compare expression profiles between P3 and P14 BMSC. Quantitative PCR was employed to validate the microarray results. Results: P3 BMSC expressed genes involved in neural developmental events such as glial differentiation, neuron proliferation, and neurite formation. They also express genes encoding for growth factors and for proteins involved in growth factor signaling. A total of 6687 genes were co-expressed in P3 and P14 BMSC. Of these co-expressed genes, 3% (202 genes) was differentially expressed with 159 genes higher in P3 BMSC and 43 genes higher in P14 BMSC. The gene expression patterns were independently validated using quantitative PCR. Functional data mining by Gene Ontology (GO)-analysis revealed that 85/159 and 22/43 genes were annotated in the GO database. In P3 BMSC, 53 GO-classes were overrepresented with several involved in organ development, cell proliferation, and neural repair. In P14 BMSC, three GO-classes were overrepresented with one involved in organ development. Conclusions: Our gene profiling results suggested a decreased plasticity and repair aptitude of long-term cultured BMSC. Our data indicated the use of early passage BMSC for neural repair approaches.

Original languageEnglish (US)
Pages (from-to)187-201
Number of pages15
JournalRestorative Neurology and Neuroscience
Volume29
Issue number3
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Nervous System
Genes
Gene Ontology
Cultured Cells
Intercellular Signaling Peptides and Proteins
Gene Expression
Polymerase Chain Reaction
Data Mining
Gene Expression Profiling
Neurites
Transcriptome
Neuroglia
Cell Proliferation
Genome
Databases
Neurons

Keywords

  • BMSC
  • cell culture
  • gene profiling
  • microarray
  • stem cells

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Clinical Neurology

Cite this

Early passage bone marrow stromal cells express genes involved in nervous system development supporting their relevance for neural repair. / Tewarie, R. D S Nandoe; Bossers, K.; Ritfeld, G. J.; Blits, B.; Grotenhuis, J. A.; Verhaagen, J.; Oudega, Martin.

In: Restorative Neurology and Neuroscience, Vol. 29, No. 3, 2011, p. 187-201.

Research output: Contribution to journalArticle

Tewarie, R. D S Nandoe ; Bossers, K. ; Ritfeld, G. J. ; Blits, B. ; Grotenhuis, J. A. ; Verhaagen, J. ; Oudega, Martin. / Early passage bone marrow stromal cells express genes involved in nervous system development supporting their relevance for neural repair. In: Restorative Neurology and Neuroscience. 2011 ; Vol. 29, No. 3. pp. 187-201.
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