Abstract
Cell-mediated early immune recognition of xenogeneic vascularized discordant grafts is poorly characterized. It has been the purpose of our studies to elucidate the role of lymphocytes in the recognition and rejection phenomena. To this end, we have utilized both ex vivo and in vitro model systems. We demonstrate that selected human lymphocyte subpopulations (mainly NK cells) rapidly and specifically adhere to xenogeneic endothelia. Efficient lysis of endothelial cells is subsequently mediated by such a population. Adhesion and cytotoxicity occur via at least two pathways, one dependent on and the other independent of the presence of human natural antibodies of the G class. Both IgG-dependent and IgG-independent adhesion and cytotoxicity can be partly inhibited by the use of anti-leukocyte integrin monoclonal antibodies. IgG-dependent adhesion and cytotoxicity can be also partly inhibited by carbohydrate structures that contain the α-galactosyl epitope. A possible role of these events in the eventual outcome of discordant vascularized xenogeneic transplants can be postulated.
| Original language | English |
|---|---|
| Pages (from-to) | 71-93 |
| Number of pages | 23 |
| Journal | Immunological Reviews |
| Issue number | 141 |
| State | Published - Oct 1 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Early events in cell-mediated recognition of vascularized xenografts : Cooperative interactions between selected lymphocyte subsets and natural antibodies. / Inverardi, Luca A; Pardi, Ruggero.
In: Immunological Reviews, No. 141, 01.10.1994, p. 71-93.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Early events in cell-mediated recognition of vascularized xenografts
T2 - Cooperative interactions between selected lymphocyte subsets and natural antibodies
AU - Inverardi, Luca A
AU - Pardi, Ruggero
PY - 1994/10/1
Y1 - 1994/10/1
N2 - Cell-mediated early immune recognition of xenogeneic vascularized discordant grafts is poorly characterized. It has been the purpose of our studies to elucidate the role of lymphocytes in the recognition and rejection phenomena. To this end, we have utilized both ex vivo and in vitro model systems. We demonstrate that selected human lymphocyte subpopulations (mainly NK cells) rapidly and specifically adhere to xenogeneic endothelia. Efficient lysis of endothelial cells is subsequently mediated by such a population. Adhesion and cytotoxicity occur via at least two pathways, one dependent on and the other independent of the presence of human natural antibodies of the G class. Both IgG-dependent and IgG-independent adhesion and cytotoxicity can be partly inhibited by the use of anti-leukocyte integrin monoclonal antibodies. IgG-dependent adhesion and cytotoxicity can be also partly inhibited by carbohydrate structures that contain the α-galactosyl epitope. A possible role of these events in the eventual outcome of discordant vascularized xenogeneic transplants can be postulated.
AB - Cell-mediated early immune recognition of xenogeneic vascularized discordant grafts is poorly characterized. It has been the purpose of our studies to elucidate the role of lymphocytes in the recognition and rejection phenomena. To this end, we have utilized both ex vivo and in vitro model systems. We demonstrate that selected human lymphocyte subpopulations (mainly NK cells) rapidly and specifically adhere to xenogeneic endothelia. Efficient lysis of endothelial cells is subsequently mediated by such a population. Adhesion and cytotoxicity occur via at least two pathways, one dependent on and the other independent of the presence of human natural antibodies of the G class. Both IgG-dependent and IgG-independent adhesion and cytotoxicity can be partly inhibited by the use of anti-leukocyte integrin monoclonal antibodies. IgG-dependent adhesion and cytotoxicity can be also partly inhibited by carbohydrate structures that contain the α-galactosyl epitope. A possible role of these events in the eventual outcome of discordant vascularized xenogeneic transplants can be postulated.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028171345&partnerID=8YFLogxK
M3 - Article
C2 - 7868158
AN - SCOPUS:0028171345
SP - 71
EP - 93
JO - Immunological Reviews
JF - Immunological Reviews
SN - 0105-2896
IS - 141
ER -