Early events in calcium-induced liposome fusion

Douglas C. Miller, Gerhard Dahl

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Calcium-induced interaction of liposomes composed of pure phosphatidylserine (PS) has been studied using a rapid-mixing, rapid-freeze device. Freeze-fracture electron microscopy of this material revealed that liposomes react very rapidly after addition of calcium ions. After only 10 ms (the resolution of the technique) vesicle fusion was apparent. At the same time, however, vesicles also collapsed, and appeared as aggregates of flattened membranes. This may explain controversies which have arisen over vesicle fusion studied with more indirect methods.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalBBA - Biomembranes
Volume689
Issue number1
DOIs
StatePublished - Jul 14 1982

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Liposomes
Fusion reactions
Calcium
Phosphatidylserines
Electron microscopy
Electron Microscopy
Ions
Membranes
Equipment and Supplies

Keywords

  • (Stopped flow/rapid freeze technique)
  • Ca dependence
  • Liposome
  • Membrane fusion
  • Phosphatidylserine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

Early events in calcium-induced liposome fusion. / Miller, Douglas C.; Dahl, Gerhard.

In: BBA - Biomembranes, Vol. 689, No. 1, 14.07.1982, p. 165-169.

Research output: Contribution to journalArticle

Miller, Douglas C. ; Dahl, Gerhard. / Early events in calcium-induced liposome fusion. In: BBA - Biomembranes. 1982 ; Vol. 689, No. 1. pp. 165-169.
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