Early cytokine and chemokine gene expression in lymph nodes of macaques infected with simian immunodeficiency virus is predictive of disease outcome and vaccine efficacy

Weiping Zou, Andrew A. Lackner, Meredith Simon, Ingrid Durand-Gasselin, Pierre Galanaud, Ronald Charles Desrosiers, Dominique Emilie

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Competitive PCR was used to evaluate the expression of cytokine, granzyme B, and chemokine genes in lymph nudes of macaques recently infected with the simian immunodeficiency virus (SIV) pathogenic molecular clone SIVmac239 (n = 16), the nonpathogenic vaccine strain SIVmac239Δnef (n = 8), and the nonpathogenic molecular clone SIVmac1A11 (n = 8). For both SIVmac239 and its nef-deleted derivative, strong expression was observed as early as 7 days postinfection for interleukin 1β (IL-1β), IL-6, tumor necrosis factor alpha, gamma interferon, and IL-13. The levels of gene induction were equally intense for both viruses despite a lower viral load for SIVmac239Δnef compared with that for SIVmac239. However, the nature of the cytokine network activation varied with the viral inocula. Primary infection with SIVmac239 was characterized by a higher level of IL-4, IL-10, MIP-1α, MIP-1β, MCP-1, and RANTES gene expression and a lower level of IL-12 and granzyme B gene expression compared with infection with SIVmac239Δnef. Thus, infection with nef-deleted SIV was associated with a preferential Th1 versus Th2 pattern of cytokine production. Infection with SIVmac1A11 was characterized by a delayed immune response for all markers tested. The unique patterns of cytokine and chemokine gene expression in lymph nodes correlated nicely with the pathogenic potential of the SIV strains used as well as with differences in their ability to serve as protective vaccines.

Original languageEnglish (US)
Pages (from-to)1227-1236
Number of pages10
JournalJournal of Virology
Volume71
Issue number2
StatePublished - Feb 1997
Externally publishedYes

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca
chemokines
Chemokines
lymph nodes
cytokines
Vaccines
Lymph Nodes
Cytokines
vaccines
Gene Expression
Granzymes
gene expression
Infection
infection
Clone Cells
clones
Chemokine CCL5
Interleukin-13

ASJC Scopus subject areas

  • Immunology

Cite this

Early cytokine and chemokine gene expression in lymph nodes of macaques infected with simian immunodeficiency virus is predictive of disease outcome and vaccine efficacy. / Zou, Weiping; Lackner, Andrew A.; Simon, Meredith; Durand-Gasselin, Ingrid; Galanaud, Pierre; Desrosiers, Ronald Charles; Emilie, Dominique.

In: Journal of Virology, Vol. 71, No. 2, 02.1997, p. 1227-1236.

Research output: Contribution to journalArticle

Zou, Weiping ; Lackner, Andrew A. ; Simon, Meredith ; Durand-Gasselin, Ingrid ; Galanaud, Pierre ; Desrosiers, Ronald Charles ; Emilie, Dominique. / Early cytokine and chemokine gene expression in lymph nodes of macaques infected with simian immunodeficiency virus is predictive of disease outcome and vaccine efficacy. In: Journal of Virology. 1997 ; Vol. 71, No. 2. pp. 1227-1236.
@article{b5732e8b06404f04ae0ea2f74e379385,
title = "Early cytokine and chemokine gene expression in lymph nodes of macaques infected with simian immunodeficiency virus is predictive of disease outcome and vaccine efficacy",
abstract = "Competitive PCR was used to evaluate the expression of cytokine, granzyme B, and chemokine genes in lymph nudes of macaques recently infected with the simian immunodeficiency virus (SIV) pathogenic molecular clone SIVmac239 (n = 16), the nonpathogenic vaccine strain SIVmac239Δnef (n = 8), and the nonpathogenic molecular clone SIVmac1A11 (n = 8). For both SIVmac239 and its nef-deleted derivative, strong expression was observed as early as 7 days postinfection for interleukin 1β (IL-1β), IL-6, tumor necrosis factor alpha, gamma interferon, and IL-13. The levels of gene induction were equally intense for both viruses despite a lower viral load for SIVmac239Δnef compared with that for SIVmac239. However, the nature of the cytokine network activation varied with the viral inocula. Primary infection with SIVmac239 was characterized by a higher level of IL-4, IL-10, MIP-1α, MIP-1β, MCP-1, and RANTES gene expression and a lower level of IL-12 and granzyme B gene expression compared with infection with SIVmac239Δnef. Thus, infection with nef-deleted SIV was associated with a preferential Th1 versus Th2 pattern of cytokine production. Infection with SIVmac1A11 was characterized by a delayed immune response for all markers tested. The unique patterns of cytokine and chemokine gene expression in lymph nodes correlated nicely with the pathogenic potential of the SIV strains used as well as with differences in their ability to serve as protective vaccines.",
author = "Weiping Zou and Lackner, {Andrew A.} and Meredith Simon and Ingrid Durand-Gasselin and Pierre Galanaud and Desrosiers, {Ronald Charles} and Dominique Emilie",
year = "1997",
month = "2",
language = "English (US)",
volume = "71",
pages = "1227--1236",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Early cytokine and chemokine gene expression in lymph nodes of macaques infected with simian immunodeficiency virus is predictive of disease outcome and vaccine efficacy

AU - Zou, Weiping

AU - Lackner, Andrew A.

AU - Simon, Meredith

AU - Durand-Gasselin, Ingrid

AU - Galanaud, Pierre

AU - Desrosiers, Ronald Charles

AU - Emilie, Dominique

PY - 1997/2

Y1 - 1997/2

N2 - Competitive PCR was used to evaluate the expression of cytokine, granzyme B, and chemokine genes in lymph nudes of macaques recently infected with the simian immunodeficiency virus (SIV) pathogenic molecular clone SIVmac239 (n = 16), the nonpathogenic vaccine strain SIVmac239Δnef (n = 8), and the nonpathogenic molecular clone SIVmac1A11 (n = 8). For both SIVmac239 and its nef-deleted derivative, strong expression was observed as early as 7 days postinfection for interleukin 1β (IL-1β), IL-6, tumor necrosis factor alpha, gamma interferon, and IL-13. The levels of gene induction were equally intense for both viruses despite a lower viral load for SIVmac239Δnef compared with that for SIVmac239. However, the nature of the cytokine network activation varied with the viral inocula. Primary infection with SIVmac239 was characterized by a higher level of IL-4, IL-10, MIP-1α, MIP-1β, MCP-1, and RANTES gene expression and a lower level of IL-12 and granzyme B gene expression compared with infection with SIVmac239Δnef. Thus, infection with nef-deleted SIV was associated with a preferential Th1 versus Th2 pattern of cytokine production. Infection with SIVmac1A11 was characterized by a delayed immune response for all markers tested. The unique patterns of cytokine and chemokine gene expression in lymph nodes correlated nicely with the pathogenic potential of the SIV strains used as well as with differences in their ability to serve as protective vaccines.

AB - Competitive PCR was used to evaluate the expression of cytokine, granzyme B, and chemokine genes in lymph nudes of macaques recently infected with the simian immunodeficiency virus (SIV) pathogenic molecular clone SIVmac239 (n = 16), the nonpathogenic vaccine strain SIVmac239Δnef (n = 8), and the nonpathogenic molecular clone SIVmac1A11 (n = 8). For both SIVmac239 and its nef-deleted derivative, strong expression was observed as early as 7 days postinfection for interleukin 1β (IL-1β), IL-6, tumor necrosis factor alpha, gamma interferon, and IL-13. The levels of gene induction were equally intense for both viruses despite a lower viral load for SIVmac239Δnef compared with that for SIVmac239. However, the nature of the cytokine network activation varied with the viral inocula. Primary infection with SIVmac239 was characterized by a higher level of IL-4, IL-10, MIP-1α, MIP-1β, MCP-1, and RANTES gene expression and a lower level of IL-12 and granzyme B gene expression compared with infection with SIVmac239Δnef. Thus, infection with nef-deleted SIV was associated with a preferential Th1 versus Th2 pattern of cytokine production. Infection with SIVmac1A11 was characterized by a delayed immune response for all markers tested. The unique patterns of cytokine and chemokine gene expression in lymph nodes correlated nicely with the pathogenic potential of the SIV strains used as well as with differences in their ability to serve as protective vaccines.

UR - http://www.scopus.com/inward/record.url?scp=0031014644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031014644&partnerID=8YFLogxK

M3 - Article

VL - 71

SP - 1227

EP - 1236

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 2

ER -