E. Coli growth inhibition by a high copy number derivative of plasmid pBR322

Manuel S. Valenzuela, Emeka V. Ikpeazu, K. A.I. Siddiqui

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We have observed that plasmid pKH47, a pBR322-derivative containing a 100bp poly(dA)-poly(dT) insertion, causes growth inhibition of host E, coli cells harboring it. In this paper we show that tills inhibitory effect is due to an increased copy number property of this plasmid, which in turn lends to an over expression of tile plasmid-encoded tet gene. Our work also indicates that contrary to other pleiotropic effects caused by the let gene product, which solely depend on the expression of the 5' end of the gene, growth inhibition requires an intact tet gene. In addition we present the isolation of an E. coli mutant that is refractive to the inhibitory effect of pKH47 and shares some properties with the parental bacteria containing plasmid pKH4.

Original languageEnglish (US)
Pages (from-to)876-883
Number of pages8
JournalBiochemical and biophysical research communications
Volume219
Issue number3
DOIs
StatePublished - Feb 27 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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