E-cadherin-dependent growth suppression is mediated by the cyclin- dependent kinase inhibitor p27(KIP1)

Brad St. Croix, Capucine Sheehan, Janusz W. Rak, Vivi Ann Fłorenes, Joyce M. Slingerland, Robert S. Kerbel

Research output: Contribution to journalArticlepeer-review

388 Scopus citations

Abstract

Recent studies have demonstrated the importance of E-cadherin, a homophilic cell-cell adhesion molecule, in contact inhibition of growth of normal epithelial cells. Many tumor cells also maintain strong intercellular adhesion, and are growth-inhibited by cell-cell contact, especially when grown in three-dimensional culture. To determine if E-cadherin could mediate contact-dependent growth inhibition of nonadherent EMT/6 mouse mammary carcinoma cells that lack E-cadherin, we transfected these cells with an exogenous E-cadherin expression vector. E-cadherin expression in EMT/6 cells resulted in tighter adhesion of multicellular spheroids and a reduced proliferative fraction in three-dimensional culture. In addition to increased cell-cell adhesion, E-cadherin expression also resulted in dephosphorylation of the retinoblastoma protein, an increase in the level of the cyclin- dependent kinase inhibitor p27(kip1) and a late reduction in cyclin D1 protein. Tightly adherent spheroids also showed increased levels of p27 bound to the cyclin E-cdk2 complex, and a reduction in cyclin E-cdk2 activity. Exposure to E-cadherin-neutralizing antibodies in three-dimensional culture simultaneously prevented adhesion and stimulated proliferation of E-cadherin transfectants as well as a panel of human colon, breast, and lung carcinoma cell lines that express functional E-cadherin. To test the importance of p27 in E-cadherin-dependent growth inhibition, we engineered E-cadherin-positive cells to express inducible p27. By forcing expression of p27 levels similar to those observed in aggregated cells, the stimulatory effect of E-cadherin- neutralizing antibodies on proliferation could be inhibited. This study demonstrates that E-cadherin, classically described as an invasion suppressor, is also a major growth suppressor, and its ability to inhibit proliferation involves upregulation of the cyclin-dependent kinase inhibitor p27.

Original languageEnglish (US)
Pages (from-to)557-571
Number of pages15
JournalJournal of Cell Biology
Volume142
Issue number2
DOIs
StatePublished - Jul 27 1998
Externally publishedYes

Keywords

  • Cyclin D
  • E-cadherin
  • Epidermal growth factor receptor
  • Kip1
  • P27

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'E-cadherin-dependent growth suppression is mediated by the cyclin- dependent kinase inhibitor p27(KIP1)'. Together they form a unique fingerprint.

Cite this