Dysregulation of B7.2 (CD86) expression on monocytes of HIV-infected individuals is associated with altered production of IL-2

A. Kumar, J. B. Angels, S. Aucoin, W. D. Creery, M. P. Daftarian, D. W. Cameron, I. Filion, F. Diaz-Mitoma

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

T helper (Th) responses are mediated in part by immunoregulatory cytokines and the signals delivered by the costimulatory CD28-B7 pathway. In this study, we have investigated the relationship between the regulation of B7 isoform expression on antigen-presenting cells from HIV+ individuals and the production of Th cytokines. The level of expression of both B7.1 and B7.2 isoforms as measured by mean channel fluorescence was significantly decreased on freshly isolated monocytes from HIV+ individuals compared with HIV- controls. However, the levels of expression of B7.1 and B7.2 on both B cells and monocytes increased significantly following culture in HIV+ individuals compared with HIV- controls. B7 expression is subject to regulation by immunoregulatory cytokines. Therefore, we analysed the regulation of B7 expression by cytokines, namely IL-10 and tumour necrosis factor-alpha (TNF- α), the production of which is enhanced in HIV infection and have similar inhibitory effects on B7 expression. Two groups of HIV+ individuals were distinguished on the basis of the inhibitory effect of IL-10 and TNF-α on monocyte B7.2 expression. IL-10 inhibited B7.2 expression on monocytes from some HIV+ individuals (termed responders) like the HIV- controls. However, in a subset of HIV+ individuals (non-responders) this inhibitory effect was lost. Loss of inhibition of B7.2 expression by IL-10 was associated with significantly reduced IL-2 production by phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC). These observations showing an association of B7 dysregulation on monocytes and B cells with altered production of IL-2 may have implications in HIV immunopathogenesis.

Original languageEnglish (US)
Pages (from-to)84-91
Number of pages8
JournalClinical and Experimental Immunology
Volume117
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Interleukin-2
Monocytes
HIV
Interleukin-10
Cytokines
Protein Isoforms
B-Lymphocytes
Tumor Necrosis Factor-alpha
Phytohemagglutinins
Antigen-Presenting Cells
HIV Infections
Blood Cells
Fluorescence

Keywords

  • B7
  • Cytokines
  • HIV-1

ASJC Scopus subject areas

  • Immunology

Cite this

Dysregulation of B7.2 (CD86) expression on monocytes of HIV-infected individuals is associated with altered production of IL-2. / Kumar, A.; Angels, J. B.; Aucoin, S.; Creery, W. D.; Daftarian, M. P.; Cameron, D. W.; Filion, I.; Diaz-Mitoma, F.

In: Clinical and Experimental Immunology, Vol. 117, No. 1, 1999, p. 84-91.

Research output: Contribution to journalArticle

Kumar, A, Angels, JB, Aucoin, S, Creery, WD, Daftarian, MP, Cameron, DW, Filion, I & Diaz-Mitoma, F 1999, 'Dysregulation of B7.2 (CD86) expression on monocytes of HIV-infected individuals is associated with altered production of IL-2', Clinical and Experimental Immunology, vol. 117, no. 1, pp. 84-91. https://doi.org/10.1046/j.1365-2249.1999.00937.x
Kumar, A. ; Angels, J. B. ; Aucoin, S. ; Creery, W. D. ; Daftarian, M. P. ; Cameron, D. W. ; Filion, I. ; Diaz-Mitoma, F. / Dysregulation of B7.2 (CD86) expression on monocytes of HIV-infected individuals is associated with altered production of IL-2. In: Clinical and Experimental Immunology. 1999 ; Vol. 117, No. 1. pp. 84-91.
@article{52e88794a7e94c30b9559c8d4b8ab0b1,
title = "Dysregulation of B7.2 (CD86) expression on monocytes of HIV-infected individuals is associated with altered production of IL-2",
abstract = "T helper (Th) responses are mediated in part by immunoregulatory cytokines and the signals delivered by the costimulatory CD28-B7 pathway. In this study, we have investigated the relationship between the regulation of B7 isoform expression on antigen-presenting cells from HIV+ individuals and the production of Th cytokines. The level of expression of both B7.1 and B7.2 isoforms as measured by mean channel fluorescence was significantly decreased on freshly isolated monocytes from HIV+ individuals compared with HIV- controls. However, the levels of expression of B7.1 and B7.2 on both B cells and monocytes increased significantly following culture in HIV+ individuals compared with HIV- controls. B7 expression is subject to regulation by immunoregulatory cytokines. Therefore, we analysed the regulation of B7 expression by cytokines, namely IL-10 and tumour necrosis factor-alpha (TNF- α), the production of which is enhanced in HIV infection and have similar inhibitory effects on B7 expression. Two groups of HIV+ individuals were distinguished on the basis of the inhibitory effect of IL-10 and TNF-α on monocyte B7.2 expression. IL-10 inhibited B7.2 expression on monocytes from some HIV+ individuals (termed responders) like the HIV- controls. However, in a subset of HIV+ individuals (non-responders) this inhibitory effect was lost. Loss of inhibition of B7.2 expression by IL-10 was associated with significantly reduced IL-2 production by phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC). These observations showing an association of B7 dysregulation on monocytes and B cells with altered production of IL-2 may have implications in HIV immunopathogenesis.",
keywords = "B7, Cytokines, HIV-1",
author = "A. Kumar and Angels, {J. B.} and S. Aucoin and Creery, {W. D.} and Daftarian, {M. P.} and Cameron, {D. W.} and I. Filion and F. Diaz-Mitoma",
year = "1999",
doi = "10.1046/j.1365-2249.1999.00937.x",
language = "English (US)",
volume = "117",
pages = "84--91",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Dysregulation of B7.2 (CD86) expression on monocytes of HIV-infected individuals is associated with altered production of IL-2

AU - Kumar, A.

AU - Angels, J. B.

AU - Aucoin, S.

AU - Creery, W. D.

AU - Daftarian, M. P.

AU - Cameron, D. W.

AU - Filion, I.

AU - Diaz-Mitoma, F.

PY - 1999

Y1 - 1999

N2 - T helper (Th) responses are mediated in part by immunoregulatory cytokines and the signals delivered by the costimulatory CD28-B7 pathway. In this study, we have investigated the relationship between the regulation of B7 isoform expression on antigen-presenting cells from HIV+ individuals and the production of Th cytokines. The level of expression of both B7.1 and B7.2 isoforms as measured by mean channel fluorescence was significantly decreased on freshly isolated monocytes from HIV+ individuals compared with HIV- controls. However, the levels of expression of B7.1 and B7.2 on both B cells and monocytes increased significantly following culture in HIV+ individuals compared with HIV- controls. B7 expression is subject to regulation by immunoregulatory cytokines. Therefore, we analysed the regulation of B7 expression by cytokines, namely IL-10 and tumour necrosis factor-alpha (TNF- α), the production of which is enhanced in HIV infection and have similar inhibitory effects on B7 expression. Two groups of HIV+ individuals were distinguished on the basis of the inhibitory effect of IL-10 and TNF-α on monocyte B7.2 expression. IL-10 inhibited B7.2 expression on monocytes from some HIV+ individuals (termed responders) like the HIV- controls. However, in a subset of HIV+ individuals (non-responders) this inhibitory effect was lost. Loss of inhibition of B7.2 expression by IL-10 was associated with significantly reduced IL-2 production by phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC). These observations showing an association of B7 dysregulation on monocytes and B cells with altered production of IL-2 may have implications in HIV immunopathogenesis.

AB - T helper (Th) responses are mediated in part by immunoregulatory cytokines and the signals delivered by the costimulatory CD28-B7 pathway. In this study, we have investigated the relationship between the regulation of B7 isoform expression on antigen-presenting cells from HIV+ individuals and the production of Th cytokines. The level of expression of both B7.1 and B7.2 isoforms as measured by mean channel fluorescence was significantly decreased on freshly isolated monocytes from HIV+ individuals compared with HIV- controls. However, the levels of expression of B7.1 and B7.2 on both B cells and monocytes increased significantly following culture in HIV+ individuals compared with HIV- controls. B7 expression is subject to regulation by immunoregulatory cytokines. Therefore, we analysed the regulation of B7 expression by cytokines, namely IL-10 and tumour necrosis factor-alpha (TNF- α), the production of which is enhanced in HIV infection and have similar inhibitory effects on B7 expression. Two groups of HIV+ individuals were distinguished on the basis of the inhibitory effect of IL-10 and TNF-α on monocyte B7.2 expression. IL-10 inhibited B7.2 expression on monocytes from some HIV+ individuals (termed responders) like the HIV- controls. However, in a subset of HIV+ individuals (non-responders) this inhibitory effect was lost. Loss of inhibition of B7.2 expression by IL-10 was associated with significantly reduced IL-2 production by phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC). These observations showing an association of B7 dysregulation on monocytes and B cells with altered production of IL-2 may have implications in HIV immunopathogenesis.

KW - B7

KW - Cytokines

KW - HIV-1

UR - http://www.scopus.com/inward/record.url?scp=0033007506&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033007506&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2249.1999.00937.x

DO - 10.1046/j.1365-2249.1999.00937.x

M3 - Article

VL - 117

SP - 84

EP - 91

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 1

ER -