Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal organization, and neuronal morphology

Tatiana I. Slepak, Lindsey D. Salay, Vance Lemmon, John Bixby

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In a neuronal overexpression screen focused on kinases and phosphatases, one "hit" was the dual specificity tyrosine phosphorylation-regulated kinase (Dyrk4), which increased the number of dendritic branches in hippocampal neurons. Overexpression of various Dyrk family members in primary neurons significantly changed neuronal morphology. Dyrk1A decreased axon growth, Dyrk3 and Dyrk4 increased dendritic branching, and Dyrk2 decreased both axon and dendrite growth and branching. Kinase-deficient mutants revealed that most of these effects depend on kinase activity. Because doublecortin (DCX), a microtubule-binding protein, regulates cytoskeletal dynamics and neuronal morphogenesis, we investigated the possibility that DCX is a target of Dyrks. We found that overexpression of Dyrk2 and Dyrk3, but not Dyrk1A or Dyrk4, can change DCX phosphorylation status. Mutation of a consensus phosphorylation site for Dyrk kinases at Serine 306 (Ser306) in DCX indicated that this is one target site for Dyrk2 and Dyrk3. Overexpression of Dyrk2 restored altered DCX distribution in the growth cones of dendrites and axons, and partially reversed the morphological effects of DCX overexpression; some of these effects were abrogated by mutation of Ser306 to alanine. These studies implicate Dyrks in the regulation of cytoskeletal organization and process outgrowth in neurons, and suggest that DCX is one relevant Dyrk target.

Original languageEnglish
Pages (from-to)514-527
Number of pages14
JournalCytoskeleton
Volume69
Issue number7
DOIs
StatePublished - Jul 1 2012

Fingerprint

Axons
Phosphotransferases
Phosphorylation
Dendrites
Neurons
Serine
Microtubule Proteins
Growth Cones
Mutation
Growth
Morphogenesis
Phosphoric Monoester Hydrolases
Alanine
Carrier Proteins
Dyrk kinase

Keywords

  • Actin
  • Axon growth
  • Dendrite branching
  • Microtubules

ASJC Scopus subject areas

  • Cell Biology
  • Structural Biology

Cite this

Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal organization, and neuronal morphology. / Slepak, Tatiana I.; Salay, Lindsey D.; Lemmon, Vance; Bixby, John.

In: Cytoskeleton, Vol. 69, No. 7, 01.07.2012, p. 514-527.

Research output: Contribution to journalArticle

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