We report our recent introduction of a dynamic spectral imaging technique into a microscope system for specimen examination and classification. It uses a dynamic spectral filter and a diffraction grating on an intermediate image plane for fast spectral image acquisition that only captures interested spectral image frames. The reconfigurable capability enables the optimal spectral band selection for a specific application without hardware component modification. It can achieve 8 nm fine spectral resolution in the whole visible spectral band suitable for fluorescent image evaluation. With a fast spectral imaging update, it can support large quantify specimen screening and classification.
|Original language||English (US)|
|Journal||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|State||Published - Jun 1 2009|
|Event||Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII - San Jose, CA, United States|
Duration: Jan 26 2009 → Jan 28 2009
- Fourier transform.
- Spectral imaging
- Spectral zoom
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging