Dynamic reconfigurable spectral imaging microscopy

Bing Chen, Jame J. Yang, Michael Renxun Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report our recent introduction of a dynamic spectral imaging technique into a microscope system for specimen examination and classification. It uses a dynamic spectral filter and a diffraction grating on an intermediate image plane for fast spectral image acquisition that only captures interested spectral image frames. The reconfigurable capability enables the optimal spectral band selection for a specific application without hardware component modification. It can achieve 8 nm fine spectral resolution in the whole visible spectral band suitable for fluorescent image evaluation. With a fast spectral imaging update, it can support large quantify specimen screening and classification.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7182
DOIs
StatePublished - 2009
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII - San Jose, CA, United States
Duration: Jan 26 2009Jan 28 2009

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
CountryUnited States
CitySan Jose, CA
Period1/26/091/28/09

Fingerprint

Spectral Imaging
Microscopy
Microscopic examination
microscopy
Imaging techniques
Image acquisition
Spectral resolution
Diffraction gratings
spectral bands
Screening
Microscopes
Hardware
gratings (spectra)
Diffraction Grating
imaging techniques
spectral resolution
Image Acquisition
Spectral Resolution
acquisition
hardware

Keywords

  • Fourier transform.
  • Microscope
  • Spectral imaging
  • Spectral zoom

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Chen, B., Yang, J. J., & Wang, M. R. (2009). Dynamic reconfigurable spectral imaging microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7182). [71821D] https://doi.org/10.1117/12.810548

Dynamic reconfigurable spectral imaging microscopy. / Chen, Bing; Yang, Jame J.; Wang, Michael Renxun.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7182 2009. 71821D.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chen, B, Yang, JJ & Wang, MR 2009, Dynamic reconfigurable spectral imaging microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7182, 71821D, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, San Jose, CA, United States, 1/26/09. https://doi.org/10.1117/12.810548
Chen B, Yang JJ, Wang MR. Dynamic reconfigurable spectral imaging microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7182. 2009. 71821D https://doi.org/10.1117/12.810548
Chen, Bing ; Yang, Jame J. ; Wang, Michael Renxun. / Dynamic reconfigurable spectral imaging microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7182 2009.
@inproceedings{eb6e117529e74be488ae634387f3c08f,
title = "Dynamic reconfigurable spectral imaging microscopy",
abstract = "We report our recent introduction of a dynamic spectral imaging technique into a microscope system for specimen examination and classification. It uses a dynamic spectral filter and a diffraction grating on an intermediate image plane for fast spectral image acquisition that only captures interested spectral image frames. The reconfigurable capability enables the optimal spectral band selection for a specific application without hardware component modification. It can achieve 8 nm fine spectral resolution in the whole visible spectral band suitable for fluorescent image evaluation. With a fast spectral imaging update, it can support large quantify specimen screening and classification.",
keywords = "Fourier transform., Microscope, Spectral imaging, Spectral zoom",
author = "Bing Chen and Yang, {Jame J.} and Wang, {Michael Renxun}",
year = "2009",
doi = "10.1117/12.810548",
language = "English (US)",
volume = "7182",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Dynamic reconfigurable spectral imaging microscopy

AU - Chen, Bing

AU - Yang, Jame J.

AU - Wang, Michael Renxun

PY - 2009

Y1 - 2009

N2 - We report our recent introduction of a dynamic spectral imaging technique into a microscope system for specimen examination and classification. It uses a dynamic spectral filter and a diffraction grating on an intermediate image plane for fast spectral image acquisition that only captures interested spectral image frames. The reconfigurable capability enables the optimal spectral band selection for a specific application without hardware component modification. It can achieve 8 nm fine spectral resolution in the whole visible spectral band suitable for fluorescent image evaluation. With a fast spectral imaging update, it can support large quantify specimen screening and classification.

AB - We report our recent introduction of a dynamic spectral imaging technique into a microscope system for specimen examination and classification. It uses a dynamic spectral filter and a diffraction grating on an intermediate image plane for fast spectral image acquisition that only captures interested spectral image frames. The reconfigurable capability enables the optimal spectral band selection for a specific application without hardware component modification. It can achieve 8 nm fine spectral resolution in the whole visible spectral band suitable for fluorescent image evaluation. With a fast spectral imaging update, it can support large quantify specimen screening and classification.

KW - Fourier transform.

KW - Microscope

KW - Spectral imaging

KW - Spectral zoom

UR - http://www.scopus.com/inward/record.url?scp=65949095640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65949095640&partnerID=8YFLogxK

U2 - 10.1117/12.810548

DO - 10.1117/12.810548

M3 - Conference contribution

VL - 7182

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -