Dynamic modulation of gelatin methacrylate hydrogel stiffness for functional maturation of human stem cell-derived cardiac tissues

Gulistan Tansik, Ahmad Alassaf, Vera Mayo, Diana Velluto, Ashutosh Agarwal

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Cardiomyocytes derived from human embryonic stem cells (hESCs) or induced pluripotent stem cells appear to be a promising cell source for practical applications like cell transplantation for myocardial repair, cardiotoxicity testing during drug development, and patient-specific disease modeling 1,2 . However, these cells exhibit a fetal phenotype and are functionally immature compared to cardiomyocytes in adult hearts. Maturation of stem cell derived cardiomyocyte can be promoted in a microenvironment that mimics physiologically relevant, dynamic extracellular matrix (ECM) changes in cardiac tissues 3,4 . Myocardial ECM stiffness increases during cardiac development 5 . Biomaterial platforms can be dynamically modulated to mimic this mechanical ECM change 4 . Importantly, gelatin is derived from collagen and does not require any additional extracellular matrix linkage steps to facilitate cell adhesion, and its stiffness can be tuned within physiological ranges 6 . The aim of this study is to develop a biomimetic two-dimensional gelatin-based hydrogel system, which stiffens temporally, for the maturation of hESC-derived cardiomyocytes. Engineering mature cardiac tissue from stem cell-derived cardiomyocytes would allow future development of improved drug testing platforms, disease models, and regenerative medicine therapies.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

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    Tansik, G., Alassaf, A., Mayo, V., Velluto, D., & Agarwal, A. (2019). Dynamic modulation of gelatin methacrylate hydrogel stiffness for functional maturation of human stem cell-derived cardiac tissues. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.