Abstract
The recruitment of T lymphocytes to lymphoid organs or sites of inflammation is a crucial step in adaptive immunity. These processes require endothelial activation and expression of adhesion molecules, including E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, the complete characterization of the adhesion strength and dynamics between lymphocytes and endothelial cells has been hampered by the lack of sensitive quantitative techniques. Here we report on the application of atomic force microscopy to characterize the interaction between individual pairs of living T lymphocytes (i.e., Jurkat cells) and human umbilical vein endothelial cells (HUVECs). The detachment of individual cell-cell conjugates was a complex process involving several step-like rupture events and the viscoelastic deformation of cells on the scale of several microns. Adhesion between Jurkat cells and activated endothelial cells increased with compression force and contact time, with the most dramatic changes occurring within the first half second of contact. After 0.25 sec of contact, E-selectin, ICAM-1, and VCAM-1 contributed to 18%, 39%, and 41% of total adhesion strength, respectively, suggesting that ICAM-1 and VCAM-1 contributed more than the selectins in supporting cell attachment.
| Original language | English |
|---|---|
| Pages (from-to) | 1306-1312 |
| Number of pages | 7 |
| Journal | Experimental Biology and Medicine |
| Volume | 231 |
| Issue number | 8 |
| State | Published - Sep 11 2006 |
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Keywords
- Atomic force microscopy
- Cell adhesion
- T lymphocyte
- Vascular endothelial cell
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Dynamic adhesion of T lymphocytes to endothelial cells revealed by atomic force microscopy. / Zhang, Xiaohui; Wojcikiewicz, Ewa P.; Moy, Vincent T.
In: Experimental Biology and Medicine, Vol. 231, No. 8, 11.09.2006, p. 1306-1312.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Dynamic adhesion of T lymphocytes to endothelial cells revealed by atomic force microscopy
AU - Zhang, Xiaohui
AU - Wojcikiewicz, Ewa P.
AU - Moy, Vincent T.
PY - 2006/9/11
Y1 - 2006/9/11
N2 - The recruitment of T lymphocytes to lymphoid organs or sites of inflammation is a crucial step in adaptive immunity. These processes require endothelial activation and expression of adhesion molecules, including E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, the complete characterization of the adhesion strength and dynamics between lymphocytes and endothelial cells has been hampered by the lack of sensitive quantitative techniques. Here we report on the application of atomic force microscopy to characterize the interaction between individual pairs of living T lymphocytes (i.e., Jurkat cells) and human umbilical vein endothelial cells (HUVECs). The detachment of individual cell-cell conjugates was a complex process involving several step-like rupture events and the viscoelastic deformation of cells on the scale of several microns. Adhesion between Jurkat cells and activated endothelial cells increased with compression force and contact time, with the most dramatic changes occurring within the first half second of contact. After 0.25 sec of contact, E-selectin, ICAM-1, and VCAM-1 contributed to 18%, 39%, and 41% of total adhesion strength, respectively, suggesting that ICAM-1 and VCAM-1 contributed more than the selectins in supporting cell attachment.
AB - The recruitment of T lymphocytes to lymphoid organs or sites of inflammation is a crucial step in adaptive immunity. These processes require endothelial activation and expression of adhesion molecules, including E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, the complete characterization of the adhesion strength and dynamics between lymphocytes and endothelial cells has been hampered by the lack of sensitive quantitative techniques. Here we report on the application of atomic force microscopy to characterize the interaction between individual pairs of living T lymphocytes (i.e., Jurkat cells) and human umbilical vein endothelial cells (HUVECs). The detachment of individual cell-cell conjugates was a complex process involving several step-like rupture events and the viscoelastic deformation of cells on the scale of several microns. Adhesion between Jurkat cells and activated endothelial cells increased with compression force and contact time, with the most dramatic changes occurring within the first half second of contact. After 0.25 sec of contact, E-selectin, ICAM-1, and VCAM-1 contributed to 18%, 39%, and 41% of total adhesion strength, respectively, suggesting that ICAM-1 and VCAM-1 contributed more than the selectins in supporting cell attachment.
KW - Atomic force microscopy
KW - Cell adhesion
KW - T lymphocyte
KW - Vascular endothelial cell
UR - http://www.scopus.com/inward/record.url?scp=33748308762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748308762&partnerID=8YFLogxK
M3 - Article
C2 - 16946399
AN - SCOPUS:33748308762
VL - 231
SP - 1306
EP - 1312
JO - Experimental Biology and Medicine
JF - Experimental Biology and Medicine
SN - 0037-9727
IS - 8
ER -