Dynamic adhesion of T lymphocytes to endothelial cells revealed by atomic force microscopy

Xiaohui Zhang, Ewa P. Wojcikiewicz, Vincent T. Moy

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The recruitment of T lymphocytes to lymphoid organs or sites of inflammation is a crucial step in adaptive immunity. These processes require endothelial activation and expression of adhesion molecules, including E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, the complete characterization of the adhesion strength and dynamics between lymphocytes and endothelial cells has been hampered by the lack of sensitive quantitative techniques. Here we report on the application of atomic force microscopy to characterize the interaction between individual pairs of living T lymphocytes (i.e., Jurkat cells) and human umbilical vein endothelial cells (HUVECs). The detachment of individual cell-cell conjugates was a complex process involving several step-like rupture events and the viscoelastic deformation of cells on the scale of several microns. Adhesion between Jurkat cells and activated endothelial cells increased with compression force and contact time, with the most dramatic changes occurring within the first half second of contact. After 0.25 sec of contact, E-selectin, ICAM-1, and VCAM-1 contributed to 18%, 39%, and 41% of total adhesion strength, respectively, suggesting that ICAM-1 and VCAM-1 contributed more than the selectins in supporting cell attachment.

Original languageEnglish
Pages (from-to)1306-1312
Number of pages7
JournalExperimental Biology and Medicine
Volume231
Issue number8
StatePublished - Sep 11 2006

Fingerprint

T-cells
Vascular Cell Adhesion Molecule-1
Atomic Force Microscopy
Endothelial cells
Intercellular Adhesion Molecule-1
Atomic force microscopy
E-Selectin
Adhesion
Endothelial Cells
Bond strength (materials)
T-Lymphocytes
Jurkat Cells
Selectins
P-Selectin
Lymphocytes
Human Umbilical Vein Endothelial Cells
Adaptive Immunity
Chemical activation
Rupture
Molecules

Keywords

  • Atomic force microscopy
  • Cell adhesion
  • T lymphocyte
  • Vascular endothelial cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Dynamic adhesion of T lymphocytes to endothelial cells revealed by atomic force microscopy. / Zhang, Xiaohui; Wojcikiewicz, Ewa P.; Moy, Vincent T.

In: Experimental Biology and Medicine, Vol. 231, No. 8, 11.09.2006, p. 1306-1312.

Research output: Contribution to journalArticle

@article{9baf23ac3a03417fb8eb3e0925e70793,
title = "Dynamic adhesion of T lymphocytes to endothelial cells revealed by atomic force microscopy",
abstract = "The recruitment of T lymphocytes to lymphoid organs or sites of inflammation is a crucial step in adaptive immunity. These processes require endothelial activation and expression of adhesion molecules, including E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, the complete characterization of the adhesion strength and dynamics between lymphocytes and endothelial cells has been hampered by the lack of sensitive quantitative techniques. Here we report on the application of atomic force microscopy to characterize the interaction between individual pairs of living T lymphocytes (i.e., Jurkat cells) and human umbilical vein endothelial cells (HUVECs). The detachment of individual cell-cell conjugates was a complex process involving several step-like rupture events and the viscoelastic deformation of cells on the scale of several microns. Adhesion between Jurkat cells and activated endothelial cells increased with compression force and contact time, with the most dramatic changes occurring within the first half second of contact. After 0.25 sec of contact, E-selectin, ICAM-1, and VCAM-1 contributed to 18{\%}, 39{\%}, and 41{\%} of total adhesion strength, respectively, suggesting that ICAM-1 and VCAM-1 contributed more than the selectins in supporting cell attachment.",
keywords = "Atomic force microscopy, Cell adhesion, T lymphocyte, Vascular endothelial cell",
author = "Xiaohui Zhang and Wojcikiewicz, {Ewa P.} and Moy, {Vincent T.}",
year = "2006",
month = "9",
day = "11",
language = "English",
volume = "231",
pages = "1306--1312",
journal = "Experimental Biology and Medicine",
issn = "0037-9727",
publisher = "Society for Experimental Biology and Medicine",
number = "8",

}

TY - JOUR

T1 - Dynamic adhesion of T lymphocytes to endothelial cells revealed by atomic force microscopy

AU - Zhang, Xiaohui

AU - Wojcikiewicz, Ewa P.

AU - Moy, Vincent T.

PY - 2006/9/11

Y1 - 2006/9/11

N2 - The recruitment of T lymphocytes to lymphoid organs or sites of inflammation is a crucial step in adaptive immunity. These processes require endothelial activation and expression of adhesion molecules, including E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, the complete characterization of the adhesion strength and dynamics between lymphocytes and endothelial cells has been hampered by the lack of sensitive quantitative techniques. Here we report on the application of atomic force microscopy to characterize the interaction between individual pairs of living T lymphocytes (i.e., Jurkat cells) and human umbilical vein endothelial cells (HUVECs). The detachment of individual cell-cell conjugates was a complex process involving several step-like rupture events and the viscoelastic deformation of cells on the scale of several microns. Adhesion between Jurkat cells and activated endothelial cells increased with compression force and contact time, with the most dramatic changes occurring within the first half second of contact. After 0.25 sec of contact, E-selectin, ICAM-1, and VCAM-1 contributed to 18%, 39%, and 41% of total adhesion strength, respectively, suggesting that ICAM-1 and VCAM-1 contributed more than the selectins in supporting cell attachment.

AB - The recruitment of T lymphocytes to lymphoid organs or sites of inflammation is a crucial step in adaptive immunity. These processes require endothelial activation and expression of adhesion molecules, including E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, the complete characterization of the adhesion strength and dynamics between lymphocytes and endothelial cells has been hampered by the lack of sensitive quantitative techniques. Here we report on the application of atomic force microscopy to characterize the interaction between individual pairs of living T lymphocytes (i.e., Jurkat cells) and human umbilical vein endothelial cells (HUVECs). The detachment of individual cell-cell conjugates was a complex process involving several step-like rupture events and the viscoelastic deformation of cells on the scale of several microns. Adhesion between Jurkat cells and activated endothelial cells increased with compression force and contact time, with the most dramatic changes occurring within the first half second of contact. After 0.25 sec of contact, E-selectin, ICAM-1, and VCAM-1 contributed to 18%, 39%, and 41% of total adhesion strength, respectively, suggesting that ICAM-1 and VCAM-1 contributed more than the selectins in supporting cell attachment.

KW - Atomic force microscopy

KW - Cell adhesion

KW - T lymphocyte

KW - Vascular endothelial cell

UR - http://www.scopus.com/inward/record.url?scp=33748308762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748308762&partnerID=8YFLogxK

M3 - Article

C2 - 16946399

AN - SCOPUS:33748308762

VL - 231

SP - 1306

EP - 1312

JO - Experimental Biology and Medicine

JF - Experimental Biology and Medicine

SN - 0037-9727

IS - 8

ER -