Dual effect of glucose on cytoplasmic free Ca2+ concentration and insulin release reflects the β-cell being deprived of fuel

Thomas Nilsson, Per Arkhammar, Per Olof Berggren

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Influence of basal glucose concentration on the response evoked by subsequent stimulation with the sugar, was evaluated by investigating changes in free cytoplasmic Ca2+ concentration, [Ca2+]i, and insulin release, using β-cells isolated from obese hyperglycemic mice. When increasing the glucose concentration from 0 to either 11 or 20 mM, there was a transient decrease in both [Ca2+]i and insulin release. The decrease was followed by a pronounced increase in both of the parameters. When increasing the basal glucose concentration, the initial decrease gradually disappeared, being abolished already at 5 mM of the sugar and the subsequent increase appeared more rapidly. It is suggested that the observed decrease in [Ca2+]i and thereby insulin release reflects a phenomenon associated with fuel deprived β-cells.

Original languageEnglish (US)
Pages (from-to)984-991
Number of pages8
JournalBiochemical and biophysical research communications
Volume153
Issue number3
DOIs
StatePublished - Jun 30 1988

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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