Dual effect of glucose on cytoplasmic free Ca2+ concentration and insulin release reflects the β-cell being deprived of fuel

Thomas Nilsson, Per Arkhammar, Per Olof Berggren

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Influence of basal glucose concentration on the response evoked by subsequent stimulation with the sugar, was evaluated by investigating changes in free cytoplasmic Ca2+ concentration, [Ca2+]i, and insulin release, using β-cells isolated from obese hyperglycemic mice. When increasing the glucose concentration from 0 to either 11 or 20 mM, there was a transient decrease in both [Ca2+]i and insulin release. The decrease was followed by a pronounced increase in both of the parameters. When increasing the basal glucose concentration, the initial decrease gradually disappeared, being abolished already at 5 mM of the sugar and the subsequent increase appeared more rapidly. It is suggested that the observed decrease in [Ca2+]i and thereby insulin release reflects a phenomenon associated with fuel deprived β-cells.

Original languageEnglish (US)
Pages (from-to)984-991
Number of pages8
JournalBiochemical and biophysical research communications
Volume153
Issue number3
DOIs
StatePublished - Jun 30 1988

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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