D2O and the sodium pump in squid nerve membrane

D. Landowne

doi:10.1007/BF01869309

D₂O and the sodium pump in squid nerve membrane

D. Landowne

Physiology & Biophysics

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

In 10 K artificial seawater (ASW). D₂O replacement reduced the Na efflux of squid axons by about one third. In 0 K ASW, D₂O replacement had little effect. D₂O reduced the K⁺ sensitivity of the efllux but increased the affinity for K⁺. A 4° decrease in temperature mimicked the effects of D₂O. When axons were injected with arginine, to decrease the ATP/ADP ratio, they lost K⁺ sensitivity in normal ASW, as expected. Their efflux into 0 K ASW became D₂O sensitive. The results are discussed in terms of conformational changes in the Na pump molecular complex.

Original language	English (US)
Pages (from-to)	277-281
Number of pages	5
Journal	The Journal of Membrane Biology
Volume	96
Issue number	3
DOIs	https://doi.org/10.1007/BF01869309
State	Published - Oct 1 1987

Keywords

deuterium oxide
heavy water

ASJC Scopus subject areas

Physiology
Cell Biology
Biophysics

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10.1007/BF01869309

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abstract = "In 10 K artificial seawater (ASW). D2O replacement reduced the Na efflux of squid axons by about one third. In 0 K ASW, D2O replacement had little effect. D2O reduced the K+ sensitivity of the efllux but increased the affinity for K+. A 4° decrease in temperature mimicked the effects of D2O. When axons were injected with arginine, to decrease the ATP/ADP ratio, they lost K+ sensitivity in normal ASW, as expected. Their efflux into 0 K ASW became D2O sensitive. The results are discussed in terms of conformational changes in the Na pump molecular complex.",

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N2 - In 10 K artificial seawater (ASW). D2O replacement reduced the Na efflux of squid axons by about one third. In 0 K ASW, D2O replacement had little effect. D2O reduced the K+ sensitivity of the efllux but increased the affinity for K+. A 4° decrease in temperature mimicked the effects of D2O. When axons were injected with arginine, to decrease the ATP/ADP ratio, they lost K+ sensitivity in normal ASW, as expected. Their efflux into 0 K ASW became D2O sensitive. The results are discussed in terms of conformational changes in the Na pump molecular complex.

AB - In 10 K artificial seawater (ASW). D2O replacement reduced the Na efflux of squid axons by about one third. In 0 K ASW, D2O replacement had little effect. D2O reduced the K+ sensitivity of the efllux but increased the affinity for K+. A 4° decrease in temperature mimicked the effects of D2O. When axons were injected with arginine, to decrease the ATP/ADP ratio, they lost K+ sensitivity in normal ASW, as expected. Their efflux into 0 K ASW became D2O sensitive. The results are discussed in terms of conformational changes in the Na pump molecular complex.

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