D2O and the sodium pump in squid nerve membrane

David Landowne

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In 10 K artificial seawater (ASW). D2O replacement reduced the Na efflux of squid axons by about one third. In 0 K ASW, D2O replacement had little effect. D2O reduced the K+ sensitivity of the efllux but increased the affinity for K+. A 4° decrease in temperature mimicked the effects of D2O. When axons were injected with arginine, to decrease the ATP/ADP ratio, they lost K+ sensitivity in normal ASW, as expected. Their efflux into 0 K ASW became D2O sensitive. The results are discussed in terms of conformational changes in the Na pump molecular complex.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalThe Journal of Membrane Biology
Volume96
Issue number3
DOIs
StatePublished - Oct 1 1987

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Sodium-Potassium-Exchanging ATPase
Decapodiformes
Seawater
Membranes
Axons
Adenosine Diphosphate
Arginine
Adenosine Triphosphate
Temperature

Keywords

  • deuterium oxide
  • heavy water

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

D2O and the sodium pump in squid nerve membrane. / Landowne, David.

In: The Journal of Membrane Biology, Vol. 96, No. 3, 01.10.1987, p. 277-281.

Research output: Contribution to journalArticle

Landowne, David. / D2O and the sodium pump in squid nerve membrane. In: The Journal of Membrane Biology. 1987 ; Vol. 96, No. 3. pp. 277-281.
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