Abstract
In 10 K artificial seawater (ASW). D2O replacement reduced the Na efflux of squid axons by about one third. In 0 K ASW, D2O replacement had little effect. D2O reduced the K+ sensitivity of the efllux but increased the affinity for K+. A 4° decrease in temperature mimicked the effects of D2O. When axons were injected with arginine, to decrease the ATP/ADP ratio, they lost K+ sensitivity in normal ASW, as expected. Their efflux into 0 K ASW became D2O sensitive. The results are discussed in terms of conformational changes in the Na pump molecular complex.
| Original language | English |
|---|---|
| Pages (from-to) | 277-281 |
| Number of pages | 5 |
| Journal | The Journal of Membrane Biology |
| Volume | 96 |
| Issue number | 3 |
| DOIs | |
| State | Published - Oct 1 1987 |
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Keywords
- deuterium oxide
- heavy water
ASJC Scopus subject areas
- Physiology
- Cell Biology
- Biophysics
Cite this
D2O and the sodium pump in squid nerve membrane. / Landowne, David.
In: The Journal of Membrane Biology, Vol. 96, No. 3, 01.10.1987, p. 277-281.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - D2O and the sodium pump in squid nerve membrane
AU - Landowne, David
PY - 1987/10/1
Y1 - 1987/10/1
N2 - In 10 K artificial seawater (ASW). D2O replacement reduced the Na efflux of squid axons by about one third. In 0 K ASW, D2O replacement had little effect. D2O reduced the K+ sensitivity of the efllux but increased the affinity for K+. A 4° decrease in temperature mimicked the effects of D2O. When axons were injected with arginine, to decrease the ATP/ADP ratio, they lost K+ sensitivity in normal ASW, as expected. Their efflux into 0 K ASW became D2O sensitive. The results are discussed in terms of conformational changes in the Na pump molecular complex.
AB - In 10 K artificial seawater (ASW). D2O replacement reduced the Na efflux of squid axons by about one third. In 0 K ASW, D2O replacement had little effect. D2O reduced the K+ sensitivity of the efllux but increased the affinity for K+. A 4° decrease in temperature mimicked the effects of D2O. When axons were injected with arginine, to decrease the ATP/ADP ratio, they lost K+ sensitivity in normal ASW, as expected. Their efflux into 0 K ASW became D2O sensitive. The results are discussed in terms of conformational changes in the Na pump molecular complex.
KW - deuterium oxide
KW - heavy water
UR - http://www.scopus.com/inward/record.url?scp=0023159965&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023159965&partnerID=8YFLogxK
U2 - 10.1007/BF01869309
DO - 10.1007/BF01869309
M3 - Article
VL - 96
SP - 277
EP - 281
JO - Journal of Membrane Biology
JF - Journal of Membrane Biology
SN - 0022-2631
IS - 3
ER -