Drug-resistant severe acanthamoeba keratitis caused by rare T5 acanthamoeba genotype

Alfonso Iovieno, Rafael A. Oechsler, Dolena R. Ledee, Darlene Miller, Eduardo C Alfonso

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: To describe a case of severe and drug-resistant Acanthamoeba keratitis in a contact lens wearer caused by atypical T5 Acanthamoeba genotype (Acanthamoeba lenticulata). Methods: Report of a case, Acanthamoeba DNA amplification and sequencing. Results: A 61-year-old patient was referred to our clinic with a 2-week history of keratitis. Acanthamoeba keratitis (AK) was diagnosed using confocal microscopy and corneal scraping culture. Using polymerase chain reaction (PCR) and DNA sequencing, the organism was classified as a T5 genotype (A.lenticulata). The keratitis continued to progress despite topical antiamoebic therapy and ultimately led to enucleation of the affected eye. Conclusions: T5 genotype Acanthamoeba can cause severe AK. Atypical Acanthamoeba genotypes could be associated with worse prognosis and resistance to therapy.

Original languageEnglish
Pages (from-to)183-184
Number of pages2
JournalEye and Contact Lens
Volume36
Issue number3
DOIs
StatePublished - May 1 2010

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Acanthamoeba Keratitis
Acanthamoeba
Genotype
Pharmaceutical Preparations
Keratitis
DNA Sequence Analysis
Eye Enucleation
Contact Lenses
Confocal Microscopy
Polymerase Chain Reaction
Therapeutics

ASJC Scopus subject areas

  • Ophthalmology

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Drug-resistant severe acanthamoeba keratitis caused by rare T5 acanthamoeba genotype. / Iovieno, Alfonso; Oechsler, Rafael A.; Ledee, Dolena R.; Miller, Darlene; Alfonso, Eduardo C.

In: Eye and Contact Lens, Vol. 36, No. 3, 01.05.2010, p. 183-184.

Research output: Contribution to journalArticle

Iovieno, Alfonso ; Oechsler, Rafael A. ; Ledee, Dolena R. ; Miller, Darlene ; Alfonso, Eduardo C. / Drug-resistant severe acanthamoeba keratitis caused by rare T5 acanthamoeba genotype. In: Eye and Contact Lens. 2010 ; Vol. 36, No. 3. pp. 183-184.
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