Doxorubicin retention and chemoresistance in human mesothelioma cell lines

Hiroshi Isobe; Larry Wellham; Antonieta Sauerteig; Kasi S. Sridhar; Cheppail Ramachandran; Awtar Krishan

doi:10.1002/ijc.2910570423

Doxorubicin retention and chemoresistance in human mesothelioma cell lines

Hiroshi Isobe, Larry Wellham, Antonieta Sauerteig, Kasi S. Sridhar, Cheppail Ramachandran, Awtar Krishan

Pathology

Research output: Contribution to journal › Article

12 Scopus citations

Abstract

Eight cell lines were established from the pleural effusion of 4 patients with malignant mesothelioma. The most sensitive (FCCMES-4) and the most resistant (FCCMES-2) mesothelioma cell lines had IC₅₀ of 0.66 and 1.85 μM for doxorubicin in clonogenic assays, respectively. In comparison with murine leukemic P388 cells, mesothelioma cell lines were 7.5- to 21-fold more resistant to doxorubicin. Co-incubation with verapamil significantly increased doxorubicin retention in one of the cell lines (FCCMES-2) expressing P-glycoprotein in 16.8% of the cells. These results indicate that doxorubicin resistance may be intrinsic in refractory mesothelioma patients and P-glycoprotein-mediated drug efflux may be involved in resistance of some of the mesotheliomas.

Original language	English (US)
Pages (from-to)	581-585
Number of pages	5
Journal	International Journal of Cancer
Volume	57
Issue number	4
DOIs	https://doi.org/10.1002/ijc.2910570423
State	Published - May 15 1994

ASJC Scopus subject areas

Oncology
Cancer Research

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Isobe, H., Wellham, L., Sauerteig, A., Sridhar, K. S., Ramachandran, C., & Krishan, A. (1994). Doxorubicin retention and chemoresistance in human mesothelioma cell lines. International Journal of Cancer, 57(4), 581-585. https://doi.org/10.1002/ijc.2910570423

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abstract = "Eight cell lines were established from the pleural effusion of 4 patients with malignant mesothelioma. The most sensitive (FCCMES-4) and the most resistant (FCCMES-2) mesothelioma cell lines had IC50 of 0.66 and 1.85 μM for doxorubicin in clonogenic assays, respectively. In comparison with murine leukemic P388 cells, mesothelioma cell lines were 7.5- to 21-fold more resistant to doxorubicin. Co-incubation with verapamil significantly increased doxorubicin retention in one of the cell lines (FCCMES-2) expressing P-glycoprotein in 16.8% of the cells. These results indicate that doxorubicin resistance may be intrinsic in refractory mesothelioma patients and P-glycoprotein-mediated drug efflux may be involved in resistance of some of the mesotheliomas.",

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