Doxorubicin retention and chemoresistance in human mesothelioma cell lines

Hiroshi Isobe, Larry Wellham, Antonieta Sauerteig, Kasi S. Sridhar, Cheppail Ramachandran, Awtar Krishan

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Eight cell lines were established from the pleural effusion of 4 patients with malignant mesothelioma. The most sensitive (FCCMES-4) and the most resistant (FCCMES-2) mesothelioma cell lines had IC50 of 0.66 and 1.85 μM for doxorubicin in clonogenic assays, respectively. In comparison with murine leukemic P388 cells, mesothelioma cell lines were 7.5- to 21-fold more resistant to doxorubicin. Co-incubation with verapamil significantly increased doxorubicin retention in one of the cell lines (FCCMES-2) expressing P-glycoprotein in 16.8% of the cells. These results indicate that doxorubicin resistance may be intrinsic in refractory mesothelioma patients and P-glycoprotein-mediated drug efflux may be involved in resistance of some of the mesotheliomas.

Original languageEnglish (US)
Pages (from-to)581-585
Number of pages5
JournalInternational Journal of Cancer
Issue number4
StatePublished - May 15 1994
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


Dive into the research topics of 'Doxorubicin retention and chemoresistance in human mesothelioma cell lines'. Together they form a unique fingerprint.

Cite this