Doxorubicin retention and chemoresistance in human mesothelioma cell lines

Hiroshi Isobe; Larry Wellham; Antonieta Sauerteig; Kasi S. Sridhar; Cheppail Ramachandran; Awtar Krishan

doi:10.1002/ijc.2910570423

Doxorubicin retention and chemoresistance in human mesothelioma cell lines

Hiroshi Isobe, Larry Wellham, Antonieta Sauerteig, Kasi S. Sridhar, Cheppail Ramachandran, Awtar Krishan

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Eight cell lines were established from the pleural effusion of 4 patients with malignant mesothelioma. The most sensitive (FCCMES-4) and the most resistant (FCCMES-2) mesothelioma cell lines had IC₅₀ of 0.66 and 1.85 μM for doxorubicin in clonogenic assays, respectively. In comparison with murine leukemic P388 cells, mesothelioma cell lines were 7.5- to 21-fold more resistant to doxorubicin. Co-incubation with verapamil significantly increased doxorubicin retention in one of the cell lines (FCCMES-2) expressing P-glycoprotein in 16.8% of the cells. These results indicate that doxorubicin resistance may be intrinsic in refractory mesothelioma patients and P-glycoprotein-mediated drug efflux may be involved in resistance of some of the mesotheliomas.

Original language	English (US)
Pages (from-to)	581-585
Number of pages	5
Journal	International Journal of Cancer
Volume	57
Issue number	4
DOIs	https://doi.org/10.1002/ijc.2910570423
State	Published - May 15 1994
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.1002/ijc.2910570423

Cite this

@article{cfde1ae4673f4dde82abc1a703a7e497,

title = "Doxorubicin retention and chemoresistance in human mesothelioma cell lines",

abstract = "Eight cell lines were established from the pleural effusion of 4 patients with malignant mesothelioma. The most sensitive (FCCMES-4) and the most resistant (FCCMES-2) mesothelioma cell lines had IC50 of 0.66 and 1.85 μM for doxorubicin in clonogenic assays, respectively. In comparison with murine leukemic P388 cells, mesothelioma cell lines were 7.5- to 21-fold more resistant to doxorubicin. Co-incubation with verapamil significantly increased doxorubicin retention in one of the cell lines (FCCMES-2) expressing P-glycoprotein in 16.8% of the cells. These results indicate that doxorubicin resistance may be intrinsic in refractory mesothelioma patients and P-glycoprotein-mediated drug efflux may be involved in resistance of some of the mesotheliomas.",

author = "Hiroshi Isobe and Larry Wellham and Antonieta Sauerteig and Sridhar, {Kasi S.} and Cheppail Ramachandran and Awtar Krishan",

year = "1994",

month = may,

day = "15",

doi = "10.1002/ijc.2910570423",

language = "English (US)",

volume = "57",

pages = "581--585",

journal = "International Journal of Cancer",

issn = "0020-7136",

publisher = "Wiley-Liss Inc.",

number = "4",

}

TY - JOUR

T1 - Doxorubicin retention and chemoresistance in human mesothelioma cell lines

AU - Isobe, Hiroshi

AU - Wellham, Larry

AU - Sauerteig, Antonieta

AU - Sridhar, Kasi S.

AU - Ramachandran, Cheppail

AU - Krishan, Awtar

PY - 1994/5/15

Y1 - 1994/5/15

N2 - Eight cell lines were established from the pleural effusion of 4 patients with malignant mesothelioma. The most sensitive (FCCMES-4) and the most resistant (FCCMES-2) mesothelioma cell lines had IC50 of 0.66 and 1.85 μM for doxorubicin in clonogenic assays, respectively. In comparison with murine leukemic P388 cells, mesothelioma cell lines were 7.5- to 21-fold more resistant to doxorubicin. Co-incubation with verapamil significantly increased doxorubicin retention in one of the cell lines (FCCMES-2) expressing P-glycoprotein in 16.8% of the cells. These results indicate that doxorubicin resistance may be intrinsic in refractory mesothelioma patients and P-glycoprotein-mediated drug efflux may be involved in resistance of some of the mesotheliomas.

AB - Eight cell lines were established from the pleural effusion of 4 patients with malignant mesothelioma. The most sensitive (FCCMES-4) and the most resistant (FCCMES-2) mesothelioma cell lines had IC50 of 0.66 and 1.85 μM for doxorubicin in clonogenic assays, respectively. In comparison with murine leukemic P388 cells, mesothelioma cell lines were 7.5- to 21-fold more resistant to doxorubicin. Co-incubation with verapamil significantly increased doxorubicin retention in one of the cell lines (FCCMES-2) expressing P-glycoprotein in 16.8% of the cells. These results indicate that doxorubicin resistance may be intrinsic in refractory mesothelioma patients and P-glycoprotein-mediated drug efflux may be involved in resistance of some of the mesotheliomas.

UR - http://www.scopus.com/inward/record.url?scp=0028285703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028285703&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910570423

DO - 10.1002/ijc.2910570423

M3 - Article

C2 - 7910154

AN - SCOPUS:0028285703

VL - 57

SP - 581

EP - 585

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -

Doxorubicin retention and chemoresistance in human mesothelioma cell lines

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this