Doxorubicin resistance in human melanoma cells

MDR-1 and glutathione S-transferase π gene expression

Cheppail Ramachandran, Kang Yuan Zhao Kang Yuan, Ling Huang Xiao Ling Huang, Awtar Krishan

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Cellular drug resistance is believed to involve P-glycoprotein-related drug efflux as well as xenobiotic detoxification. In the present study, we analyzed five human melanoma cell lines with 1- to 6-fold doxorubicin resistance for doxorubicin retention and MDR-1 and GST π gene expression. All the cell lines had high doxorubicin retention, and efflux blockers such as trifluoperazine and verapamil did not have a major effect on drug retention or cytotoxicity. Even though all the cell lines carried the MDR-1 and GST π genes, gene amplification was not associated with drug resistance. Both laser flow cytometry and immunoperoxidase staining showed high expression of C-219 reactive P-glycoprotein in some of the resistant cells which was not accompanied by either high drug efflux or sensitivity to doxorubicin efflux blockers.

Original languageEnglish
Pages (from-to)743-751
Number of pages9
JournalBiochemical Pharmacology
Volume45
Issue number3
DOIs
StatePublished - Feb 9 1993
Externally publishedYes

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Glutathione Transferase
Gene expression
Doxorubicin
Melanoma
Gene Expression
P-Glycoprotein
Drug Resistance
Cell Line
Pharmaceutical Preparations
Cells
Trifluoperazine
Gene Amplification
Xenobiotics
Verapamil
Detoxification
Flow cytometry
Cytotoxicity
Flow Cytometry
Lasers
Staining and Labeling

ASJC Scopus subject areas

  • Pharmacology

Cite this

Doxorubicin resistance in human melanoma cells : MDR-1 and glutathione S-transferase π gene expression. / Ramachandran, Cheppail; Zhao Kang Yuan, Kang Yuan; Xiao Ling Huang, Ling Huang; Krishan, Awtar.

In: Biochemical Pharmacology, Vol. 45, No. 3, 09.02.1993, p. 743-751.

Research output: Contribution to journalArticle

Ramachandran, Cheppail ; Zhao Kang Yuan, Kang Yuan ; Xiao Ling Huang, Ling Huang ; Krishan, Awtar. / Doxorubicin resistance in human melanoma cells : MDR-1 and glutathione S-transferase π gene expression. In: Biochemical Pharmacology. 1993 ; Vol. 45, No. 3. pp. 743-751.
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