TY - JOUR
T1 - Doxorubicin resistance in human melanoma cells
T2 - MDR-1 and glutathione S-transferase π gene expression
AU - Ramachandran, Cheppail
AU - Zhao Kang Yuan, Kang Yuan
AU - Xiao Ling Huang, Ling Huang
AU - Krishan, Awtar
PY - 1993/2/9
Y1 - 1993/2/9
N2 - Cellular drug resistance is believed to involve P-glycoprotein-related drug efflux as well as xenobiotic detoxification. In the present study, we analyzed five human melanoma cell lines with 1- to 6-fold doxorubicin resistance for doxorubicin retention and MDR-1 and GST π gene expression. All the cell lines had high doxorubicin retention, and efflux blockers such as trifluoperazine and verapamil did not have a major effect on drug retention or cytotoxicity. Even though all the cell lines carried the MDR-1 and GST π genes, gene amplification was not associated with drug resistance. Both laser flow cytometry and immunoperoxidase staining showed high expression of C-219 reactive P-glycoprotein in some of the resistant cells which was not accompanied by either high drug efflux or sensitivity to doxorubicin efflux blockers.
AB - Cellular drug resistance is believed to involve P-glycoprotein-related drug efflux as well as xenobiotic detoxification. In the present study, we analyzed five human melanoma cell lines with 1- to 6-fold doxorubicin resistance for doxorubicin retention and MDR-1 and GST π gene expression. All the cell lines had high doxorubicin retention, and efflux blockers such as trifluoperazine and verapamil did not have a major effect on drug retention or cytotoxicity. Even though all the cell lines carried the MDR-1 and GST π genes, gene amplification was not associated with drug resistance. Both laser flow cytometry and immunoperoxidase staining showed high expression of C-219 reactive P-glycoprotein in some of the resistant cells which was not accompanied by either high drug efflux or sensitivity to doxorubicin efflux blockers.
UR - http://www.scopus.com/inward/record.url?scp=0027396018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027396018&partnerID=8YFLogxK
U2 - 10.1016/0006-2952(93)90150-U
DO - 10.1016/0006-2952(93)90150-U
M3 - Article
C2 - 8095141
AN - SCOPUS:0027396018
VL - 45
SP - 743
EP - 751
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 3
ER -