Abstract
Cellular drug resistance is believed to involve P-glycoprotein-related drug efflux as well as xenobiotic detoxification. In the present study, we analyzed five human melanoma cell lines with 1- to 6-fold doxorubicin resistance for doxorubicin retention and MDR-1 and GST π gene expression. All the cell lines had high doxorubicin retention, and efflux blockers such as trifluoperazine and verapamil did not have a major effect on drug retention or cytotoxicity. Even though all the cell lines carried the MDR-1 and GST π genes, gene amplification was not associated with drug resistance. Both laser flow cytometry and immunoperoxidase staining showed high expression of C-219 reactive P-glycoprotein in some of the resistant cells which was not accompanied by either high drug efflux or sensitivity to doxorubicin efflux blockers.
| Original language | English |
|---|---|
| Pages (from-to) | 743-751 |
| Number of pages | 9 |
| Journal | Biochemical Pharmacology |
| Volume | 45 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 9 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pharmacology
Cite this
Doxorubicin resistance in human melanoma cells : MDR-1 and glutathione S-transferase π gene expression. / Ramachandran, Cheppail; Zhao Kang Yuan, Kang Yuan; Xiao Ling Huang, Ling Huang; Krishan, Awtar.
In: Biochemical Pharmacology, Vol. 45, No. 3, 09.02.1993, p. 743-751.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Doxorubicin resistance in human melanoma cells
T2 - MDR-1 and glutathione S-transferase π gene expression
AU - Ramachandran, Cheppail
AU - Zhao Kang Yuan, Kang Yuan
AU - Xiao Ling Huang, Ling Huang
AU - Krishan, Awtar
PY - 1993/2/9
Y1 - 1993/2/9
N2 - Cellular drug resistance is believed to involve P-glycoprotein-related drug efflux as well as xenobiotic detoxification. In the present study, we analyzed five human melanoma cell lines with 1- to 6-fold doxorubicin resistance for doxorubicin retention and MDR-1 and GST π gene expression. All the cell lines had high doxorubicin retention, and efflux blockers such as trifluoperazine and verapamil did not have a major effect on drug retention or cytotoxicity. Even though all the cell lines carried the MDR-1 and GST π genes, gene amplification was not associated with drug resistance. Both laser flow cytometry and immunoperoxidase staining showed high expression of C-219 reactive P-glycoprotein in some of the resistant cells which was not accompanied by either high drug efflux or sensitivity to doxorubicin efflux blockers.
AB - Cellular drug resistance is believed to involve P-glycoprotein-related drug efflux as well as xenobiotic detoxification. In the present study, we analyzed five human melanoma cell lines with 1- to 6-fold doxorubicin resistance for doxorubicin retention and MDR-1 and GST π gene expression. All the cell lines had high doxorubicin retention, and efflux blockers such as trifluoperazine and verapamil did not have a major effect on drug retention or cytotoxicity. Even though all the cell lines carried the MDR-1 and GST π genes, gene amplification was not associated with drug resistance. Both laser flow cytometry and immunoperoxidase staining showed high expression of C-219 reactive P-glycoprotein in some of the resistant cells which was not accompanied by either high drug efflux or sensitivity to doxorubicin efflux blockers.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027396018&partnerID=8YFLogxK
U2 - 10.1016/0006-2952(93)90150-U
DO - 10.1016/0006-2952(93)90150-U
M3 - Article
VL - 45
SP - 743
EP - 751
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 3
ER -