Down-regulation of retinoic acid receptor activity associated with decreased α and γ isoforms expression in F9 embryonal carcinoma cells differentiated by retinoic acid

D. Lazega, E. Schenker, N. Busso, Arthur Z Zelent, A. Chen, S. Waxman

Research output: Contribution to journalArticle

4 Scopus citations


F9 embryonal carcinoma cells differentiate in response to retinoic acid (RA). To investigate the regulation of RA receptors (RARs) expression during this process, cDNA probes specific for the major RAR isoforms were used. In contrast to the level of RARβ2 mRNA which was high in cells treated 5 days with RA and below detection in untreated cells, as previously described, the steady state levels of RARα1, α2, γ1, and γ2 mRNAs were markedly decreased in the RA-differentiated cells as compared to untreated cells. The down-regulation of the RA-responsive system in differentiated cells was also evident in gel shift assays as a marked decrease in binding capacity to a retinoid acid response element (β2RARE), as well as in chloramphenicol acetyltransferase (CAT) assays as a sixfold decrease in RA-mediated transacting activity via this element. The down-regulation of RAR DNA-binding and transacting activity coincided with the burst in tissue plasminogen activator secretion and thus, occurred at the hinge between early and late differentiation. The down-regulation of RA responsiveness may constitute an important event in the transition between early and late differentiation stage in F9 cells.

Original languageEnglish (US)
Pages (from-to)90-96
Number of pages7
JournalJournal of Cellular Physiology
Issue number1
StatePublished - 1993
Externally publishedYes


ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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