Down-regulation of IL-2 production by activation of T cells through Ly-6A/E

E. K. Codias, J. E. Rutter, T. J. Fleming, T. R. Malek

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21 Scopus citations

Abstract

Ly-6A/E molecules are expressed on the surface of T cells and have been shown to function in activation by the capacity of anti-Ly-6A/E mAb to induce T cell hybridomas or normal T cells to produce IL-2. Recent evidence suggests that activation through Ly-6A/E may be linked to the TCR signaling pathway. To further investigate the relationship between Ly-6- and TCR-induced T cell activation, we have examined whether an anti-Ly-6A/E mAb (D7) modulates TCR signaling in vitro. We now report that mAb D7 specifically inhibited IL-2 production by T cells also activated through TCR. Such inhibition was noted for normal T cells stimulated by soluble anti-CD3 or alloantigen and for T hybridomas stimulated by soluble anti-CD3. The ability of D7 to inhibit IL-2 production by T hybridomas was dependent on the nature of the TCR activating signal because IL-2 production was not inhibited when T hybridomas were stimulated with Ag or immobilized anti-CD3. Inhibition of IL-2 production by D7 apparently required cross-linking of the mAb because D7 F(ab′)2 fragments were not effective for inhibition of IL-2 production. Similar to its ability to enhance anti-Ly-6A/E-induced activation of T and B cells, IFN-γ enhanced the D7-induced inhibition of IL-2 production by alloantigen-activated normal T cells. These data further support the notion that Ly-6 and TCR signaling pathways are interrelated.

Original languageEnglish (US)
Pages (from-to)1407-1414
Number of pages8
JournalJournal of Immunology
Volume145
Issue number5
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Codias, E. K., Rutter, J. E., Fleming, T. J., & Malek, T. R. (1990). Down-regulation of IL-2 production by activation of T cells through Ly-6A/E. Journal of Immunology, 145(5), 1407-1414.