Down-regulation of α6 integrin, an anti-oncogene product, by functional cooperation of H-Ras and c-Myc

Hiroko Fujimoto, Yoshiya Tanaka, Zhao-Jun Liu, Hideo Yagita, Ko Okumura, Atsushi Kosugi, Akio Morinobu, Hisanori Umehara, Hirohei Yamamura, Yasuhiro Minami

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: The molecular basis of cooperation of H-Ras and c-Myc in regulating cellular behaviour, such as cell adhesiveness, is still poorly understood. To investigate the role of H-Ras and c-Myc in cell adhesiveness, a constitutively active H-RasV12 (H-RasV12) and c-Myc were stably expressed, singly or in combination in a haematopoietic cell line, and the expression and activity of cell adhesion molecules were monitored. Results: We have shown that the ectopic expression of H-RasV12, but not c-Myc alone, in a haematopoietic cell line, induces the activation of very late antigen-6 (VLA-6, α6β1) integrin. Co-expression of H-RasV12 and c-Myc in the same cells further resulted in the induction of expression of vascular cell adhesion molecule-1 (VCAM-1) and the inhibition of expression of α6 integrin, a candidate anti-oncogene product, leading to a loss of adhesiveness to laminin (Lm), a ligand for VLA-6. Conclusions: Cooperation of H-Ras and c-Myc reciprocally regulates expression of the adhesion molecules, α6 integrin and VCAM-1. Our results represent an unprecedented account of the cooperation of the oncogene products, H-Ras and c-Myc, to inhibit expression of an anti-oncogene product, α6 integrin.

Original languageEnglish
Pages (from-to)337-343
Number of pages7
JournalGenes to Cells
Volume6
Issue number4
DOIs
StatePublished - May 15 2001
Externally publishedYes

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Oncogene Proteins
Tumor Suppressor Genes
Integrins
Adhesiveness
Integrin alpha6beta1
Down-Regulation
Vascular Cell Adhesion Molecule-1
Cell Line
Cell Adhesion Molecules
Laminin
Ligands
Antigens

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Down-regulation of α6 integrin, an anti-oncogene product, by functional cooperation of H-Ras and c-Myc. / Fujimoto, Hiroko; Tanaka, Yoshiya; Liu, Zhao-Jun; Yagita, Hideo; Okumura, Ko; Kosugi, Atsushi; Morinobu, Akio; Umehara, Hisanori; Yamamura, Hirohei; Minami, Yasuhiro.

In: Genes to Cells, Vol. 6, No. 4, 15.05.2001, p. 337-343.

Research output: Contribution to journalArticle

Fujimoto, H, Tanaka, Y, Liu, Z-J, Yagita, H, Okumura, K, Kosugi, A, Morinobu, A, Umehara, H, Yamamura, H & Minami, Y 2001, 'Down-regulation of α6 integrin, an anti-oncogene product, by functional cooperation of H-Ras and c-Myc', Genes to Cells, vol. 6, no. 4, pp. 337-343. https://doi.org/10.1046/j.1365-2443.2001.00428.x
Fujimoto, Hiroko ; Tanaka, Yoshiya ; Liu, Zhao-Jun ; Yagita, Hideo ; Okumura, Ko ; Kosugi, Atsushi ; Morinobu, Akio ; Umehara, Hisanori ; Yamamura, Hirohei ; Minami, Yasuhiro. / Down-regulation of α6 integrin, an anti-oncogene product, by functional cooperation of H-Ras and c-Myc. In: Genes to Cells. 2001 ; Vol. 6, No. 4. pp. 337-343.
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AU - Fujimoto, Hiroko

AU - Tanaka, Yoshiya

AU - Liu, Zhao-Jun

AU - Yagita, Hideo

AU - Okumura, Ko

AU - Kosugi, Atsushi

AU - Morinobu, Akio

AU - Umehara, Hisanori

AU - Yamamura, Hirohei

AU - Minami, Yasuhiro

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