Double stranded scission of DNA directed through sequence-specific R-loop formation

Ralf Landgraf, Chi hong B. Chen, David S. Sigman

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

R-loop formation with short (100 nt) RNAs provides a highly flexible and stringent method to achieve sequence-specific separation of target DNA at any given sequence. After stabilization of R-loops with glyoxal and removal of the RNA through RNase treatment the remaining single-stranded DNA bubble provides a highly favorable substrate for attenuated micrococcal nuclease. We investigated this method for sequence-specific scission of double-stranded DNA and achieved quantitative scission of 3-5 kb plasmids. The applicability to larger size DNA is demonstrated through specific excision of the intervening segment between two R-loops from a P1 plasmid of ~ 120 kb.

Original languageEnglish (US)
Pages (from-to)3524-3530
Number of pages7
JournalNucleic acids research
Volume23
Issue number17
DOIs
StatePublished - Sep 11 1995

ASJC Scopus subject areas

  • Genetics

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