Double labelling to obtain S phase subpopulations: Application to determine cell kinetics of diploid cells in an aneuploid tumour

R. A. White, Alan Pollack, N. H A Terry, M. L. Meistrich, S. Cao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We studied the cell kinetics of the murine mammary carcinoma MCa-K using iododeoxyuridine (IdUrd) and chlorodeoxyuridine (CldUrd) given at different times as independently detectable labels of S phase cells. The presence of IdUrd and CldUrd, and the amount of DNA were measured by three-colour flow cytometry making it possible to define three subpopulations within S phase and to measure the progression through the cell cycle during the time following labelling. In DNA histograms of these subpopulations, the diploid and aneuploid cells (which had a DNA index of 1.7) are essentially completely separated. From appropriate combinations of cells labelled with IdUrd only, CldUrd only, or both, it was possible to construct separate DNA distributions for the labelled diploid and aneuploid cells at the times of administration of each label. The kinetics of the diploid and aneuploid cells could be calculated for individual tumours from these two time points without having to make corrections for the presence of the second population. The diploid and aneuploid populations had indistinguishable S and G2 + M phase durations, T(S) and T(G2+M), of about 9 and 2 h; however, the potential doubling time values for the aneuploid and diploid populations were 30.2 and 101.2 h respectively.

Original languageEnglish
Pages (from-to)123-137
Number of pages15
JournalCell Proliferation
Volume27
Issue number3
StatePublished - Mar 23 1994
Externally publishedYes

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Aneuploidy
Diploidy
S Phase
Idoxuridine
DNA
Neoplasms
Population
G2 Phase
Cell Division
Cell Cycle
Flow Cytometry
Color
Breast Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Double labelling to obtain S phase subpopulations : Application to determine cell kinetics of diploid cells in an aneuploid tumour. / White, R. A.; Pollack, Alan; Terry, N. H A; Meistrich, M. L.; Cao, S.

In: Cell Proliferation, Vol. 27, No. 3, 23.03.1994, p. 123-137.

Research output: Contribution to journalArticle

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