Dopamine transporter mRNA in autopsy studies of chronic cocaine users

Li Chen, David M. Segal, Carlos T. Moraes, Deborah C. Mash

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The effects of chronic cocaine abuse on transcriptional regulation of human dopamine transporter (DAT) mRNA in midbrain dopaminergic neurons was assessed by reverse transcription-polymerase chain reaction (RT-PCR). DAT/cyclophilin mRNA ratios in the substantia nigra (SN) were unchanged in cocaine overdose (CO) victims as compared to age-matched and drug-free control (CTRL) subjects. In contrast, DAT mRNA levels were decreased significantly in agitated cocaine delirium victims (67%, p < 0.05). These findings demonstrate the lack of a regulatory influence by cocaine on the steady state content of DAT mRNA in cocaine abusers. In contrast, DAT gene expression was altered significantly in cocaine abusers at risk for agitated delirium.

Original languageEnglish (US)
Pages (from-to)181-185
Number of pages5
JournalMolecular Brain Research
Volume73
Issue number1-2
DOIs
StatePublished - Nov 10 1999

Keywords

  • Cocaine
  • Cocaine delirium
  • Human brain
  • mRNA
  • Polymerase chain reaction
  • Reverse transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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