Donor Origin of Circulating Endothelial Progenitors after Allogeneic Bone Marrow Transplantation

Chukwuemeka Ikpeazu, Mari K. Davidson, Dorenda Halteman, Stacey A. Goodman, Philip J. Browning, Stephen J. Brandt

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Endothelial cell precursors circulate in blood and express antigens found on hematopoietic stem cells, suggesting that such precursors might be subject to transplantation. To investigate, we obtained adherence-depleted peripheral blood mononuclear cells from 3 individuals who had received a sex-mismatched allogeneic bone marrow transplant (BMT) and cultured the cells on fibronectin-coated plates with endothelial growth factors. The phenotype of the spindle-shaped cells that emerged in culture was characterized by immunofluorescent staining, and the origin of the cells was determined using a polymerase chain reaction (PCR)-based assay for polymorphic short tandem repeats (STRs). The cells manifested a number of endothelial characteristics - such as von Willebrand factor, CD31, and Flk-1/KDR expression; Bandeiraea simplicifolia lectin 1 binding; and acetylated low-density lipoprotein uptake - but lacked expression of certain markers of activation or differentiation, including intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and the epitope for the anti-endothelial cell antibody P1H12. For each patient and at all time points studied (ranging from 5 to 52 months after transplantation), STR-PCR analysis showed that cultured cells and nucleated blood cells came exclusively from the bone marrow donor. These results demonstrate that circulating endothelial progenitors are both transplantable and capable of long-term repopulation of human allogeneic BMT recipients.

Original languageEnglish
Pages (from-to)301-308
Number of pages8
JournalBiology of Blood and Marrow Transplantation
Volume6
Issue number3 A
StatePublished - Dec 1 2000
Externally publishedYes

Fingerprint

Homologous Transplantation
Bone Marrow Transplantation
Bone Marrow
Tissue Donors
Microsatellite Repeats
Cultured Cells
Blood Cells
Transplantation
Endothelial Growth Factors
Polymerase Chain Reaction
Vascular Cell Adhesion Molecule-1
von Willebrand Factor
Intercellular Adhesion Molecule-1
Hematopoietic Stem Cells
Fibronectins
Epitopes
Endothelial Cells
Staining and Labeling
Transplants
Phenotype

Keywords

  • Allogeneic bone marrow transplantation
  • Chimerism
  • Endothelial progenitor cells

ASJC Scopus subject areas

  • Transplantation

Cite this

Ikpeazu, C., Davidson, M. K., Halteman, D., Goodman, S. A., Browning, P. J., & Brandt, S. J. (2000). Donor Origin of Circulating Endothelial Progenitors after Allogeneic Bone Marrow Transplantation. Biology of Blood and Marrow Transplantation, 6(3 A), 301-308.

Donor Origin of Circulating Endothelial Progenitors after Allogeneic Bone Marrow Transplantation. / Ikpeazu, Chukwuemeka; Davidson, Mari K.; Halteman, Dorenda; Goodman, Stacey A.; Browning, Philip J.; Brandt, Stephen J.

In: Biology of Blood and Marrow Transplantation, Vol. 6, No. 3 A, 01.12.2000, p. 301-308.

Research output: Contribution to journalArticle

Ikpeazu, C, Davidson, MK, Halteman, D, Goodman, SA, Browning, PJ & Brandt, SJ 2000, 'Donor Origin of Circulating Endothelial Progenitors after Allogeneic Bone Marrow Transplantation', Biology of Blood and Marrow Transplantation, vol. 6, no. 3 A, pp. 301-308.
Ikpeazu, Chukwuemeka ; Davidson, Mari K. ; Halteman, Dorenda ; Goodman, Stacey A. ; Browning, Philip J. ; Brandt, Stephen J. / Donor Origin of Circulating Endothelial Progenitors after Allogeneic Bone Marrow Transplantation. In: Biology of Blood and Marrow Transplantation. 2000 ; Vol. 6, No. 3 A. pp. 301-308.
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