Does urea reabsorption occur via the glucose pathway in the kidney of the freshwater rainbow trout?

Carol Bucking, Chris M. Wood

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This study tested the hypothesis that the renal reabsorption of urea occurs via the glucose transport pathway in the freshwater rainbow trout (Oncorhynchus mykiss). The relationship between glucose transport and urea transport was examined by experimentally elevating the rate of renal glucose reabsorption via infusion of the fish with exogenous glucose, and by inactivating the glucose transporters via the administration of phlorizin. Under all treatments, urea was reabsorbed against a concentration gradient, with plasma levels of urea being higher than urine levels. Glucose was almost completely reabsorbed (88%) whereas urea was reabsorbed less efficiently (47%) but to a greater extent than water (22%). Glucose and urea reabsorption were both found to be correlated with Na+ and Cl- reabsorption, though the latter were 20 fold and 200-300 fold higher than glucose and urea transport rates, respectively. Glucose infusions greatly increased glucose reabsorption but urea reabsorption was unaffected. Phlorizin treatment completely blocked glucose reabsorption, but urea reabsorption was again unaffected. We conclude that there is no relationship between glucose and urea handling in the trout kidney, thus disproving the hypothesis.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalFish Physiology and Biochemistry
Volume30
Issue number1
DOIs
StatePublished - Mar 1 2004

Fingerprint

Oncorhynchus mykiss
Fresh Water
urea
rainbow
Urea
glucose
kidneys
Kidney
Glucose
Phlorhizin
fold
glucose transporters
Facilitative Glucose Transport Proteins
Trout
Fish
trout
urine
Fishes
Urine
Plasmas

Keywords

  • Na reabsorption
  • O.mykiss
  • Phlorizin
  • Renal function
  • Ureatransporters
  • Urine

ASJC Scopus subject areas

  • Aquatic Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Physiology
  • Physiology (medical)

Cite this

Does urea reabsorption occur via the glucose pathway in the kidney of the freshwater rainbow trout? / Bucking, Carol; Wood, Chris M.

In: Fish Physiology and Biochemistry, Vol. 30, No. 1, 01.03.2004, p. 1-12.

Research output: Contribution to journalArticle

@article{86e7208a23a24e4aa90bcf205352e976,
title = "Does urea reabsorption occur via the glucose pathway in the kidney of the freshwater rainbow trout?",
abstract = "This study tested the hypothesis that the renal reabsorption of urea occurs via the glucose transport pathway in the freshwater rainbow trout (Oncorhynchus mykiss). The relationship between glucose transport and urea transport was examined by experimentally elevating the rate of renal glucose reabsorption via infusion of the fish with exogenous glucose, and by inactivating the glucose transporters via the administration of phlorizin. Under all treatments, urea was reabsorbed against a concentration gradient, with plasma levels of urea being higher than urine levels. Glucose was almost completely reabsorbed (88{\%}) whereas urea was reabsorbed less efficiently (47{\%}) but to a greater extent than water (22{\%}). Glucose and urea reabsorption were both found to be correlated with Na+ and Cl- reabsorption, though the latter were 20 fold and 200-300 fold higher than glucose and urea transport rates, respectively. Glucose infusions greatly increased glucose reabsorption but urea reabsorption was unaffected. Phlorizin treatment completely blocked glucose reabsorption, but urea reabsorption was again unaffected. We conclude that there is no relationship between glucose and urea handling in the trout kidney, thus disproving the hypothesis.",
keywords = "Na reabsorption, O.mykiss, Phlorizin, Renal function, Ureatransporters, Urine",
author = "Carol Bucking and Wood, {Chris M.}",
year = "2004",
month = "3",
day = "1",
doi = "10.1007/s10695-004-4154-1",
language = "English",
volume = "30",
pages = "1--12",
journal = "Fish Physiology and Biochemistry",
issn = "0920-1742",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Does urea reabsorption occur via the glucose pathway in the kidney of the freshwater rainbow trout?

AU - Bucking, Carol

AU - Wood, Chris M.

PY - 2004/3/1

Y1 - 2004/3/1

N2 - This study tested the hypothesis that the renal reabsorption of urea occurs via the glucose transport pathway in the freshwater rainbow trout (Oncorhynchus mykiss). The relationship between glucose transport and urea transport was examined by experimentally elevating the rate of renal glucose reabsorption via infusion of the fish with exogenous glucose, and by inactivating the glucose transporters via the administration of phlorizin. Under all treatments, urea was reabsorbed against a concentration gradient, with plasma levels of urea being higher than urine levels. Glucose was almost completely reabsorbed (88%) whereas urea was reabsorbed less efficiently (47%) but to a greater extent than water (22%). Glucose and urea reabsorption were both found to be correlated with Na+ and Cl- reabsorption, though the latter were 20 fold and 200-300 fold higher than glucose and urea transport rates, respectively. Glucose infusions greatly increased glucose reabsorption but urea reabsorption was unaffected. Phlorizin treatment completely blocked glucose reabsorption, but urea reabsorption was again unaffected. We conclude that there is no relationship between glucose and urea handling in the trout kidney, thus disproving the hypothesis.

AB - This study tested the hypothesis that the renal reabsorption of urea occurs via the glucose transport pathway in the freshwater rainbow trout (Oncorhynchus mykiss). The relationship between glucose transport and urea transport was examined by experimentally elevating the rate of renal glucose reabsorption via infusion of the fish with exogenous glucose, and by inactivating the glucose transporters via the administration of phlorizin. Under all treatments, urea was reabsorbed against a concentration gradient, with plasma levels of urea being higher than urine levels. Glucose was almost completely reabsorbed (88%) whereas urea was reabsorbed less efficiently (47%) but to a greater extent than water (22%). Glucose and urea reabsorption were both found to be correlated with Na+ and Cl- reabsorption, though the latter were 20 fold and 200-300 fold higher than glucose and urea transport rates, respectively. Glucose infusions greatly increased glucose reabsorption but urea reabsorption was unaffected. Phlorizin treatment completely blocked glucose reabsorption, but urea reabsorption was again unaffected. We conclude that there is no relationship between glucose and urea handling in the trout kidney, thus disproving the hypothesis.

KW - Na reabsorption

KW - O.mykiss

KW - Phlorizin

KW - Renal function

KW - Ureatransporters

KW - Urine

UR - http://www.scopus.com/inward/record.url?scp=19444379201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19444379201&partnerID=8YFLogxK

U2 - 10.1007/s10695-004-4154-1

DO - 10.1007/s10695-004-4154-1

M3 - Article

AN - SCOPUS:19444379201

VL - 30

SP - 1

EP - 12

JO - Fish Physiology and Biochemistry

JF - Fish Physiology and Biochemistry

SN - 0920-1742

IS - 1

ER -