Does gill boundary layer carbonic anhydrase contribute to carbon dioxide excretion: A comparison between dogfish (Squalus acanthias) and rainbow trout (Oncorhynchus mykiss)

S. F. Perry, K. M. Gilmour, N. J. Bernier, C. M. Wood

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

In vivo experiments were conducted on spiny dogfish (Squalus acanthias) and rainbow trout (Oncorhynchus mykiss) in sea water to determine the potential role of externally oriented or gill boundary layer carbonic anhydrase in carbon dioxide excretion. This was accomplished by assessing pH changes in expired water using a stopped-flow apparatus. In dogfish, expired water was in acid-base disequilibrium as indicated by a pronounced acidification (ΔpH=-0.11±0.01; N=22; mean ± S.E.M.) during the period of stopped flow; inspired water, however, was in acid-base equilibrium (ΔpH=-0.002±0.01; N=22). The acid-base disequilibrium in expired water was abolished (ΔpH=-0.005±0.01; N=6) by the addition of bovine carbonic anhydrase (5 mg l-1) to the external medium. Addition of the carbonic anhydrase inhibitor acetazolamide (1 mmol l-1) to the water significantly reduced the magnitude of the pH disequilibrium (from -0.133±0.03 to -0.063±0.02; N=4). However, after correcting for the increased buffering capacity of the water caused by acetazolamide, the acid-base disequilibrium during stopped flow was unaffected by this treatment (control Δ[H+]=99.8±22.8 μmol l-1; acetazolamide Δ[H+]=81.3±21.5 μmol l-1). In rainbow trout, expired water displayed an acid-base disequilibrium (ΔpH=0.09±0.01; N=6) that also was abolished by the application of external carbonic anhydrase (ΔpH=0.02±0.01). The origin of the expired water acid-base disequilibrium was investigated further in dogfish. Intravascular injection of acetazolamide (40 mg kg-1) to inhibit internal carbonic anhydrase activity nonspecifically and thus CO2 excretion significantly diminished the extent of the expired water disequilibrium pH after 30 min (from -0.123±0.01 to -0.065±0.01; N=6). Selective inhibition of extracellular carbonic anhydrase activity using a low intravascular dose (1.3 mg kg-1) of the inhibitor benzolamide caused a significant reduction in the acid-base disequilibrium after 5 min (from -0.11±0.01 to -0.07±0.01; N=14). These results demonstrate that the expired water acid-base disequilibrium originates, at least in part, from excretory CO2 and that extracellular carbonic anhydrase in dogfish may have a significant role in carbon dioxide excretion. However, externally oriented carbonic anhydrase (if present in dogfish) plays no role in catalysing the hydration of the excretory CO2 in water flowing over the gills and thus is unlikely to facilitate CO2 excretion.

Original languageEnglish (US)
Pages (from-to)749-756
Number of pages8
JournalJournal of Experimental Biology
Volume202
Issue number6
StatePublished - Mar 1 1999

Keywords

  • Acetazolamide
  • Benzolamide
  • Boundary layer
  • Carbonic anhydrase
  • CO excretion
  • Dogfish
  • Gill
  • Oncorhynchus mykiss
  • Rainbow trout
  • Squalus acanthias

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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