Abstract
The intrapancreatic neuropeptide galanin potently inhibits glucose-induced insulin secretion. This effect is in part due to a repolarization of the β-cells and ensuring reduction in the cytoplasmic free Ca2+ concentration, [Ca2+]i. We propose that galanin inhibition of β-cell action potentials is associated with the appearance of ATP-regulated K+ channels. Galanin opens K+ channels in a patch membrane when applied to the external solution in the cell-attached patch configuration. However, galanin does not detectably increase K+ permeability during whole-cell experiments, even when GTP was included in the internal solution. Our findings are not consistent with a direct effect of galanin on the K+ channels, but rather indicate that the effect of the neuropeptide is mediated by some intracellular coupling factor(s).
| Original language | English |
|---|---|
| Pages (from-to) | 453-457 |
| Number of pages | 5 |
| Journal | Peptides |
| Volume | 10 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1989 |
| Externally published | Yes |
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Keywords
- Galanin
- Insulin secretion
- K channels
- β-Cells
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Cellular and Molecular Neuroscience
Cite this
Does galanin inhibit insulin secretion by opening of the ATP-regulated K+ channel in the β-cell? / Ahrén, Bo; Berggren, Per Olof; Bokvist, Krister; Rorsman, Patrik.
In: Peptides, Vol. 10, No. 2, 01.01.1989, p. 453-457.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Does galanin inhibit insulin secretion by opening of the ATP-regulated K+ channel in the β-cell?
AU - Ahrén, Bo
AU - Berggren, Per Olof
AU - Bokvist, Krister
AU - Rorsman, Patrik
PY - 1989/1/1
Y1 - 1989/1/1
N2 - The intrapancreatic neuropeptide galanin potently inhibits glucose-induced insulin secretion. This effect is in part due to a repolarization of the β-cells and ensuring reduction in the cytoplasmic free Ca2+ concentration, [Ca2+]i. We propose that galanin inhibition of β-cell action potentials is associated with the appearance of ATP-regulated K+ channels. Galanin opens K+ channels in a patch membrane when applied to the external solution in the cell-attached patch configuration. However, galanin does not detectably increase K+ permeability during whole-cell experiments, even when GTP was included in the internal solution. Our findings are not consistent with a direct effect of galanin on the K+ channels, but rather indicate that the effect of the neuropeptide is mediated by some intracellular coupling factor(s).
AB - The intrapancreatic neuropeptide galanin potently inhibits glucose-induced insulin secretion. This effect is in part due to a repolarization of the β-cells and ensuring reduction in the cytoplasmic free Ca2+ concentration, [Ca2+]i. We propose that galanin inhibition of β-cell action potentials is associated with the appearance of ATP-regulated K+ channels. Galanin opens K+ channels in a patch membrane when applied to the external solution in the cell-attached patch configuration. However, galanin does not detectably increase K+ permeability during whole-cell experiments, even when GTP was included in the internal solution. Our findings are not consistent with a direct effect of galanin on the K+ channels, but rather indicate that the effect of the neuropeptide is mediated by some intracellular coupling factor(s).
KW - Galanin
KW - Insulin secretion
KW - K channels
KW - β-Cells
UR - http://www.scopus.com/inward/record.url?scp=0024327774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024327774&partnerID=8YFLogxK
U2 - 10.1016/0196-9781(89)90058-2
DO - 10.1016/0196-9781(89)90058-2
M3 - Article
VL - 10
SP - 453
EP - 457
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 2
ER -