DNMT3B7, a truncated DNMT3B isoform expressed in human tumors, disrupts embryonic development and accelerates lymphomagenesis

Mrinal Y. Shah, Aparna Vasanthakumar, Natalie Y. Barnes, Maria E. Figueroa, Anna Kamp, Christopher Hendrick, Kelly R. Ostler, Elizabeth M. Davis, Shang Lin, John Anastasi, Michelle M. Le Beau, Ivan P. Moskowitz, Ari Melnick, Peter Pytel, Lucy A. Godley

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Epigenetic changes are among the most common alterations observed in cancer cells, yet the mechanism by which cancer cells acquire and maintain abnormal DNA methylation patterns is not understood. Cancer cells have an altered distribution of DNA methylation and express aberrant DNA methyltransferase 3B transcripts, which encode truncated proteins, some of which lack the COOH-terminal catalytic domain. To test if a truncated DNMT3B isoform disrupts DNA methylation in vivo, we constructed two lines of transgenic mice expressing DNMT3B7, a truncated DNMT3B isoform commonly found in cancer cells. DNMT3B7 transgenic mice exhibit altered embryonic development, including lymphopenia, craniofacial abnormalities, and cardiac defects, similar to Dnmt3b-deficient animals, but rarely develop cancer. However, when DNMT3B7 transgenic mice are bred with Eμ-Myc transgenic mice, which model aggressive B-cell lymphoma, DNMT3B7 expression increases the frequency of mediastinal lymphomas in Eμ-Myc animals. Eμ-Myc/DNMT3B7 mediastinal lymphomas have more chromosomal rearrangements, increased global DNA methylation levels, and more locusspecific perturbations in DNA methylation patterns compared with Eμ-Myc lymphomas. These data represent the first in vivo modeling of cancer-associated DNA methylation changes and suggest that truncated DNMT3B isoforms contribute to the redistribution of DNA methylation characterizing virtually every human tumor.

Original languageEnglish (US)
Pages (from-to)5840-5850
Number of pages11
JournalCancer Research
Volume70
Issue number14
DOIs
StatePublished - Jul 15 2010
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Shah, M. Y., Vasanthakumar, A., Barnes, N. Y., Figueroa, M. E., Kamp, A., Hendrick, C., Ostler, K. R., Davis, E. M., Lin, S., Anastasi, J., Le Beau, M. M., Moskowitz, I. P., Melnick, A., Pytel, P., & Godley, L. A. (2010). DNMT3B7, a truncated DNMT3B isoform expressed in human tumors, disrupts embryonic development and accelerates lymphomagenesis. Cancer Research, 70(14), 5840-5850. https://doi.org/10.1158/0008-5472.CAN-10-0847