DNA synthesis during lens regeneration in larval Xenopus laevis

P. R. Waggoner, R. W. Reyer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Larvae of Xenopus laevis at stages 50-53 were lentectomized and then injected with tritiated thymidine at various times after lentectomy. In Series I, the animals were injected 1, 2, 3, 4, 6, 8, 10, 12, 15, or 17 days after lens removal and fixed 3 hr after injection. Autoradiographs of serial cross sections through the eyes were prepared. Increased incorporation of thymidine in the cells of the regenerating lens were first observed 2 days (stage 2) after lentectomy. All of the cells of the lens vesicle incorporated [3H]thymidine until stage 4 at which time the cells in the inner wall of the vesicle began to differentiate into lens fibers. Labelling then became restricted to the peripheral cells (prospective lens epithelium and prospective lens fibers). At stage 5 of regeneration, only cells of the lens epithelium incorporated [3H]thymidine. In series II, animals injected 3 or 4 times after lentectomy were fixed daily from 1 to 7 days after injection. Many stage 3 and stage 4 regenerates were recovered with label throughout and stage 5 regenerates were found, 7 days after injection, with label over the lens fibers as well as over the lens epithelium.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalJournal of Experimental Zoology
Volume192
Issue number1
StatePublished - Dec 1 1975

Fingerprint

Xenopus laevis
Lens
Lenses
Regeneration
synthesis
DNA
thymidine
Thymidine
epithelium
Epithelium
injection
Injections
cells
autoradiography
Larva
animals
eyes
larvae

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

DNA synthesis during lens regeneration in larval Xenopus laevis. / Waggoner, P. R.; Reyer, R. W.

In: Journal of Experimental Zoology, Vol. 192, No. 1, 01.12.1975, p. 65-71.

Research output: Contribution to journalArticle

Waggoner, PR & Reyer, RW 1975, 'DNA synthesis during lens regeneration in larval Xenopus laevis', Journal of Experimental Zoology, vol. 192, no. 1, pp. 65-71.
Waggoner, P. R. ; Reyer, R. W. / DNA synthesis during lens regeneration in larval Xenopus laevis. In: Journal of Experimental Zoology. 1975 ; Vol. 192, No. 1. pp. 65-71.
@article{de2e4a56e8b74d38a9cf18924e66e4de,
title = "DNA synthesis during lens regeneration in larval Xenopus laevis",
abstract = "Larvae of Xenopus laevis at stages 50-53 were lentectomized and then injected with tritiated thymidine at various times after lentectomy. In Series I, the animals were injected 1, 2, 3, 4, 6, 8, 10, 12, 15, or 17 days after lens removal and fixed 3 hr after injection. Autoradiographs of serial cross sections through the eyes were prepared. Increased incorporation of thymidine in the cells of the regenerating lens were first observed 2 days (stage 2) after lentectomy. All of the cells of the lens vesicle incorporated [3H]thymidine until stage 4 at which time the cells in the inner wall of the vesicle began to differentiate into lens fibers. Labelling then became restricted to the peripheral cells (prospective lens epithelium and prospective lens fibers). At stage 5 of regeneration, only cells of the lens epithelium incorporated [3H]thymidine. In series II, animals injected 3 or 4 times after lentectomy were fixed daily from 1 to 7 days after injection. Many stage 3 and stage 4 regenerates were recovered with label throughout and stage 5 regenerates were found, 7 days after injection, with label over the lens fibers as well as over the lens epithelium.",
author = "Waggoner, {P. R.} and Reyer, {R. W.}",
year = "1975",
month = "12",
day = "1",
language = "English",
volume = "192",
pages = "65--71",
journal = "Journal of Experimental Zoology Part A: Comparative Experimental Biology",
issn = "0022-104X",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - DNA synthesis during lens regeneration in larval Xenopus laevis

AU - Waggoner, P. R.

AU - Reyer, R. W.

PY - 1975/12/1

Y1 - 1975/12/1

N2 - Larvae of Xenopus laevis at stages 50-53 were lentectomized and then injected with tritiated thymidine at various times after lentectomy. In Series I, the animals were injected 1, 2, 3, 4, 6, 8, 10, 12, 15, or 17 days after lens removal and fixed 3 hr after injection. Autoradiographs of serial cross sections through the eyes were prepared. Increased incorporation of thymidine in the cells of the regenerating lens were first observed 2 days (stage 2) after lentectomy. All of the cells of the lens vesicle incorporated [3H]thymidine until stage 4 at which time the cells in the inner wall of the vesicle began to differentiate into lens fibers. Labelling then became restricted to the peripheral cells (prospective lens epithelium and prospective lens fibers). At stage 5 of regeneration, only cells of the lens epithelium incorporated [3H]thymidine. In series II, animals injected 3 or 4 times after lentectomy were fixed daily from 1 to 7 days after injection. Many stage 3 and stage 4 regenerates were recovered with label throughout and stage 5 regenerates were found, 7 days after injection, with label over the lens fibers as well as over the lens epithelium.

AB - Larvae of Xenopus laevis at stages 50-53 were lentectomized and then injected with tritiated thymidine at various times after lentectomy. In Series I, the animals were injected 1, 2, 3, 4, 6, 8, 10, 12, 15, or 17 days after lens removal and fixed 3 hr after injection. Autoradiographs of serial cross sections through the eyes were prepared. Increased incorporation of thymidine in the cells of the regenerating lens were first observed 2 days (stage 2) after lentectomy. All of the cells of the lens vesicle incorporated [3H]thymidine until stage 4 at which time the cells in the inner wall of the vesicle began to differentiate into lens fibers. Labelling then became restricted to the peripheral cells (prospective lens epithelium and prospective lens fibers). At stage 5 of regeneration, only cells of the lens epithelium incorporated [3H]thymidine. In series II, animals injected 3 or 4 times after lentectomy were fixed daily from 1 to 7 days after injection. Many stage 3 and stage 4 regenerates were recovered with label throughout and stage 5 regenerates were found, 7 days after injection, with label over the lens fibers as well as over the lens epithelium.

UR - http://www.scopus.com/inward/record.url?scp=0016636821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016636821&partnerID=8YFLogxK

M3 - Article

C2 - 1127410

AN - SCOPUS:0016636821

VL - 192

SP - 65

EP - 71

JO - Journal of Experimental Zoology Part A: Comparative Experimental Biology

JF - Journal of Experimental Zoology Part A: Comparative Experimental Biology

SN - 0022-104X

IS - 1

ER -