DNA probes for the detection of mycoplasmas in genital specimens.

M. C. Roberts, M. Hooton, W. Stamm, K. K. Holmes, G. E. Kenny

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The utility of whole-genomic DNA probes for the detection of infections by genital mycoplasmas was investigated in 220 men attending a sexually transmitted diseases clinic. In 144 patients, probe results were compared with quantitative culture results. The prevalence of Mycoplasma hominis was 11% by culture, whereas the prevalence of ureaplasmas was 38%. The M. hominis DNA probe detected 9 of 16 M. hominis culture-positive specimens and 2 of 128 culture-negative specimens. The Ureaplasma urealyticum DNA probe detected 36 of 57 U. urealyticum culture-positive specimens and 18 of 87 culture-negative specimens. Most of the probe-negative culture-positive specimens had colony counts of less than 10(3) organisms/ml of specimen. The DNA probe does not require viable organisms, and the probe-positive, culture-negative specimens suggest that false-negative cultures occurred, perhaps due to specimen handling or insensitivity of culture methods for some strains of mycoplasmas.

Original languageEnglish (US)
Pages (from-to)618-620
Number of pages3
JournalIsrael journal of medical sciences
Volume23
Issue number6
StatePublished - Jun 1987
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering

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    Roberts, M. C., Hooton, M., Stamm, W., Holmes, K. K., & Kenny, G. E. (1987). DNA probes for the detection of mycoplasmas in genital specimens. Israel journal of medical sciences, 23(6), 618-620.