DNA patterns in parathyroid disease predict postoperative parathyroid hormone secretion

George L. Irvin, Mary Ann Taupier, Norman L Block, Eric Reiss

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Flow cytometric analysis of the nuclear deoxyribonucleic acid (DNA) content of parathyroid glands excised from patients with hypercalcemic hyperparathyroidism has identified three distinct DNA patterns. The most frequent pattern showed a high percentage of cells with tetraploid DNA, which indicated an increase in the G2 and M phase of the cell cycle. Thirty-four patients were found to have abnormal tetraploid DNA content. One patient had a normal diploid pattern, and seven were found to have an aneuploid DNA population in their excised parathyroid glands. This unexpected finding of aneuploid DNA appears to be an unique feature of these endocrine glands because they have no histologic or clinical characteristics of malignant change. All patients have remained normocalcemic and clinically well after excision of only grossly enlarged glands. Postoperative parathyroid hormone (PTH) levels were correlated in 77 patients with DNA analyses of biopsy specimens from 30 normal-sized glands which were left in situ. Seven patients with elevated PTH postoperatively had high tetraploid or aneuploid DNA in all 13 glands from which biopsy specimens had been taken. In 10 patients with normal PTH levels, six had normal diploid patterns, whereas four had high tetraploid DNA in their gland biopsy specimens. DNA content present in biopsy specimens of normal-sized, in situ glands was predictive (p < 0.042) of parathyroid gland secretory activity. These findings suggest that the stimulus for parathyroid gland hyperfunction often affects more than a single enlarged gland and persists after clinical cure, as shown by a more rapid cell turnover in some remaining glands and continued hypersecretion of hormone.

Original languageEnglish
Pages (from-to)1115-1120
Number of pages6
JournalSurgery
Volume104
Issue number6
StatePublished - Jan 1 1988

Fingerprint

Parathyroid Diseases
Parathyroid Hormone
DNA
Parathyroid Glands
Tetraploidy
Aneuploidy
Biopsy
Diploidy
Endocrine Glands
Hyperparathyroidism
G2 Phase
Cell Division
Cell Cycle

ASJC Scopus subject areas

  • Surgery

Cite this

Irvin, G. L., Taupier, M. A., Block, N. L., & Reiss, E. (1988). DNA patterns in parathyroid disease predict postoperative parathyroid hormone secretion. Surgery, 104(6), 1115-1120.

DNA patterns in parathyroid disease predict postoperative parathyroid hormone secretion. / Irvin, George L.; Taupier, Mary Ann; Block, Norman L; Reiss, Eric.

In: Surgery, Vol. 104, No. 6, 01.01.1988, p. 1115-1120.

Research output: Contribution to journalArticle

Irvin, GL, Taupier, MA, Block, NL & Reiss, E 1988, 'DNA patterns in parathyroid disease predict postoperative parathyroid hormone secretion', Surgery, vol. 104, no. 6, pp. 1115-1120.
Irvin GL, Taupier MA, Block NL, Reiss E. DNA patterns in parathyroid disease predict postoperative parathyroid hormone secretion. Surgery. 1988 Jan 1;104(6):1115-1120.
Irvin, George L. ; Taupier, Mary Ann ; Block, Norman L ; Reiss, Eric. / DNA patterns in parathyroid disease predict postoperative parathyroid hormone secretion. In: Surgery. 1988 ; Vol. 104, No. 6. pp. 1115-1120.
@article{5da1f8e27b2841a9875f43dcd92ca554,
title = "DNA patterns in parathyroid disease predict postoperative parathyroid hormone secretion",
abstract = "Flow cytometric analysis of the nuclear deoxyribonucleic acid (DNA) content of parathyroid glands excised from patients with hypercalcemic hyperparathyroidism has identified three distinct DNA patterns. The most frequent pattern showed a high percentage of cells with tetraploid DNA, which indicated an increase in the G2 and M phase of the cell cycle. Thirty-four patients were found to have abnormal tetraploid DNA content. One patient had a normal diploid pattern, and seven were found to have an aneuploid DNA population in their excised parathyroid glands. This unexpected finding of aneuploid DNA appears to be an unique feature of these endocrine glands because they have no histologic or clinical characteristics of malignant change. All patients have remained normocalcemic and clinically well after excision of only grossly enlarged glands. Postoperative parathyroid hormone (PTH) levels were correlated in 77 patients with DNA analyses of biopsy specimens from 30 normal-sized glands which were left in situ. Seven patients with elevated PTH postoperatively had high tetraploid or aneuploid DNA in all 13 glands from which biopsy specimens had been taken. In 10 patients with normal PTH levels, six had normal diploid patterns, whereas four had high tetraploid DNA in their gland biopsy specimens. DNA content present in biopsy specimens of normal-sized, in situ glands was predictive (p < 0.042) of parathyroid gland secretory activity. These findings suggest that the stimulus for parathyroid gland hyperfunction often affects more than a single enlarged gland and persists after clinical cure, as shown by a more rapid cell turnover in some remaining glands and continued hypersecretion of hormone.",
author = "Irvin, {George L.} and Taupier, {Mary Ann} and Block, {Norman L} and Eric Reiss",
year = "1988",
month = "1",
day = "1",
language = "English",
volume = "104",
pages = "1115--1120",
journal = "Surgery (United States)",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - DNA patterns in parathyroid disease predict postoperative parathyroid hormone secretion

AU - Irvin, George L.

AU - Taupier, Mary Ann

AU - Block, Norman L

AU - Reiss, Eric

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Flow cytometric analysis of the nuclear deoxyribonucleic acid (DNA) content of parathyroid glands excised from patients with hypercalcemic hyperparathyroidism has identified three distinct DNA patterns. The most frequent pattern showed a high percentage of cells with tetraploid DNA, which indicated an increase in the G2 and M phase of the cell cycle. Thirty-four patients were found to have abnormal tetraploid DNA content. One patient had a normal diploid pattern, and seven were found to have an aneuploid DNA population in their excised parathyroid glands. This unexpected finding of aneuploid DNA appears to be an unique feature of these endocrine glands because they have no histologic or clinical characteristics of malignant change. All patients have remained normocalcemic and clinically well after excision of only grossly enlarged glands. Postoperative parathyroid hormone (PTH) levels were correlated in 77 patients with DNA analyses of biopsy specimens from 30 normal-sized glands which were left in situ. Seven patients with elevated PTH postoperatively had high tetraploid or aneuploid DNA in all 13 glands from which biopsy specimens had been taken. In 10 patients with normal PTH levels, six had normal diploid patterns, whereas four had high tetraploid DNA in their gland biopsy specimens. DNA content present in biopsy specimens of normal-sized, in situ glands was predictive (p < 0.042) of parathyroid gland secretory activity. These findings suggest that the stimulus for parathyroid gland hyperfunction often affects more than a single enlarged gland and persists after clinical cure, as shown by a more rapid cell turnover in some remaining glands and continued hypersecretion of hormone.

AB - Flow cytometric analysis of the nuclear deoxyribonucleic acid (DNA) content of parathyroid glands excised from patients with hypercalcemic hyperparathyroidism has identified three distinct DNA patterns. The most frequent pattern showed a high percentage of cells with tetraploid DNA, which indicated an increase in the G2 and M phase of the cell cycle. Thirty-four patients were found to have abnormal tetraploid DNA content. One patient had a normal diploid pattern, and seven were found to have an aneuploid DNA population in their excised parathyroid glands. This unexpected finding of aneuploid DNA appears to be an unique feature of these endocrine glands because they have no histologic or clinical characteristics of malignant change. All patients have remained normocalcemic and clinically well after excision of only grossly enlarged glands. Postoperative parathyroid hormone (PTH) levels were correlated in 77 patients with DNA analyses of biopsy specimens from 30 normal-sized glands which were left in situ. Seven patients with elevated PTH postoperatively had high tetraploid or aneuploid DNA in all 13 glands from which biopsy specimens had been taken. In 10 patients with normal PTH levels, six had normal diploid patterns, whereas four had high tetraploid DNA in their gland biopsy specimens. DNA content present in biopsy specimens of normal-sized, in situ glands was predictive (p < 0.042) of parathyroid gland secretory activity. These findings suggest that the stimulus for parathyroid gland hyperfunction often affects more than a single enlarged gland and persists after clinical cure, as shown by a more rapid cell turnover in some remaining glands and continued hypersecretion of hormone.

UR - http://www.scopus.com/inward/record.url?scp=0024239783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024239783&partnerID=8YFLogxK

M3 - Article

VL - 104

SP - 1115

EP - 1120

JO - Surgery (United States)

JF - Surgery (United States)

SN - 0039-6060

IS - 6

ER -