DNA microarray analysis of cortical gene expression during early recirculation after focal brain ischemia in rat

Rainald Schmidt-Kastner, Baotong Zhang, Ludmila Belayev, Larissa Khoutorova, Rupen Amin, Raul Busto, Myron D. Ginsberg

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

Focal brain ischemia is followed by changes in gene expression as reflected by altered mRNA levels. DNA microarray analysis can be used to survey thousands of genes for differential expression triggered by ischemic metabolic stress. In this study, Sprague-Dawley rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) using an intravascular poly-L-lysine-coated filament, and brains were removed after 3 h of recirculation for mRNA isolation. A differential measurement of mRNAs from post-ischemic and sham control animals was performed using the Mouse UniGene 1 microarray. Established values for differential expression were used (≥1.7 or ≤-1.7 fold), and hits (n=2-3 arrays) divided into known 'ischemia-hypoxia response' genes and 'newly connected' annotated genes. n=28 ischemia-hypoxia response genes were up-regulated and n=6 were down-regulated. Regulated genes comprised immediate early genes, heat shock proteins, anti-oxidative enzymes, trophic factors, and genes involved in RNA metabolism, inflammation and cell signaling. Based on the ability of the microarray to replicate known changes in gene expression, n=35 newly connected genes were found up-regulated and n=41 down-regulated. DNA microarray analysis allows one to develop novel working hypotheses for responses to brain ischemia based on the regulation of annotated genes.

Original languageEnglish (US)
Pages (from-to)81-93
Number of pages13
JournalMolecular Brain Research
Volume108
Issue number1-2
DOIs
StatePublished - Dec 16 2002

Keywords

  • Gene expression
  • Ischemia
  • Microarray
  • MRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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