DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation

Rita Shaknovich, Leandro Cerchietti, Lucas Tsikitas, Matthias Kormaksson, Subhajyoti De, Maria Figueroa, Gianna Ballon, Shao Ning Yang, Nils Weinhold, Mark Reimers, Thomas Clozel, Karin Luttrop, Tomas J. Ekstrom, Jared Frank, Aparna Vasanthakumar, Lucy A. Godley, Franziska Michor, Olivier Elemento, Ari Melnick

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The phenotype of germinal center (GC) B cells includes the unique ability to tolerate rapid proliferation and the mutagenic actions of activation induced cytosine deaminase (AICDA). Given the importance of epigenetic patterning in determining cellular phenotypes, we examined DNA methylation and the role of DNA methyltransferases in the formation of GCs. DNA methylation profiling revealed a marked shift in DNA methylation patterning in GC B cells versus resting/ naive B cells. This shift included significant differential methylation of 235 genes, with concordant inverse changes in gene expression affecting most notably genes of the NFkB and MAP kinase signaling pathways. GC B cells were predominantly hypomethylated compared with naive B cells and AICDA binding sites were highly overrepresented among hypomethylated loci. GC B cells also exhibited greater DNA methylation heterogeneity than naive B cells. Among DNA methyltransferases (DNMTs), only DNMT1 was significantly up-regulated in GC B cells. Dnmt1 hypomorphic mice displayed deficient GC formation and treatment of mice with the DNA methyltransferase inhibitor decitabine resulted in failure to form GCs after immune stimulation. Notably, the GC B cells of Dnmt1 hypomorphic animals showed evidence of increased DNA damage, suggesting dual roles for DNMT1 in DNA methylation and double strand DNA break repair.

Original languageEnglish (US)
Pages (from-to)3559-3569
Number of pages11
JournalBlood
Volume118
Issue number13
DOIs
StatePublished - Sep 29 2011
Externally publishedYes

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Germinal Center
Methyltransferases
DNA Methylation
Cell Differentiation
B-Lymphocytes
Cells
DNA
Cytosine Deaminase
decitabine
Genes
Chemical activation
Phenotype
Methylation
DNA Fingerprinting
Double-Stranded DNA Breaks
MAP Kinase Signaling System
Epigenomics
Gene expression
DNA Repair
DNA Damage

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Shaknovich, R., Cerchietti, L., Tsikitas, L., Kormaksson, M., De, S., Figueroa, M., ... Melnick, A. (2011). DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation. Blood, 118(13), 3559-3569. https://doi.org/10.1182/blood-2011-06-357996

DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation. / Shaknovich, Rita; Cerchietti, Leandro; Tsikitas, Lucas; Kormaksson, Matthias; De, Subhajyoti; Figueroa, Maria; Ballon, Gianna; Yang, Shao Ning; Weinhold, Nils; Reimers, Mark; Clozel, Thomas; Luttrop, Karin; Ekstrom, Tomas J.; Frank, Jared; Vasanthakumar, Aparna; Godley, Lucy A.; Michor, Franziska; Elemento, Olivier; Melnick, Ari.

In: Blood, Vol. 118, No. 13, 29.09.2011, p. 3559-3569.

Research output: Contribution to journalArticle

Shaknovich, R, Cerchietti, L, Tsikitas, L, Kormaksson, M, De, S, Figueroa, M, Ballon, G, Yang, SN, Weinhold, N, Reimers, M, Clozel, T, Luttrop, K, Ekstrom, TJ, Frank, J, Vasanthakumar, A, Godley, LA, Michor, F, Elemento, O & Melnick, A 2011, 'DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation', Blood, vol. 118, no. 13, pp. 3559-3569. https://doi.org/10.1182/blood-2011-06-357996
Shaknovich, Rita ; Cerchietti, Leandro ; Tsikitas, Lucas ; Kormaksson, Matthias ; De, Subhajyoti ; Figueroa, Maria ; Ballon, Gianna ; Yang, Shao Ning ; Weinhold, Nils ; Reimers, Mark ; Clozel, Thomas ; Luttrop, Karin ; Ekstrom, Tomas J. ; Frank, Jared ; Vasanthakumar, Aparna ; Godley, Lucy A. ; Michor, Franziska ; Elemento, Olivier ; Melnick, Ari. / DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation. In: Blood. 2011 ; Vol. 118, No. 13. pp. 3559-3569.
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