DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation

Rita Shaknovich, Leandro Cerchietti, Lucas Tsikitas, Matthias Kormaksson, Subhajyoti De, Maria E. Figueroa, Gianna Ballon, Shao Ning Yang, Nils Weinhold, Mark Reimers, Thomas Clozel, Karin Luttrop, Tomas J. Ekstrom, Jared Frank, Aparna Vasanthakumar, Lucy A. Godley, Franziska Michor, Olivier Elemento, Ari Melnick

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


The phenotype of germinal center (GC) B cells includes the unique ability to tolerate rapid proliferation and the mutagenic actions of activation induced cytosine deaminase (AICDA). Given the importance of epigenetic patterning in determining cellular phenotypes, we examined DNA methylation and the role of DNA methyltransferases in the formation of GCs. DNA methylation profiling revealed a marked shift in DNA methylation patterning in GC B cells versus resting/ naive B cells. This shift included significant differential methylation of 235 genes, with concordant inverse changes in gene expression affecting most notably genes of the NFkB and MAP kinase signaling pathways. GC B cells were predominantly hypomethylated compared with naive B cells and AICDA binding sites were highly overrepresented among hypomethylated loci. GC B cells also exhibited greater DNA methylation heterogeneity than naive B cells. Among DNA methyltransferases (DNMTs), only DNMT1 was significantly up-regulated in GC B cells. Dnmt1 hypomorphic mice displayed deficient GC formation and treatment of mice with the DNA methyltransferase inhibitor decitabine resulted in failure to form GCs after immune stimulation. Notably, the GC B cells of Dnmt1 hypomorphic animals showed evidence of increased DNA damage, suggesting dual roles for DNMT1 in DNA methylation and double strand DNA break repair.

Original languageEnglish (US)
Pages (from-to)3559-3569
Number of pages11
Issue number13
StatePublished - Sep 29 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology


Dive into the research topics of 'DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation'. Together they form a unique fingerprint.

Cite this