DNA fragmentation follows delayed neuronal death in CA1 neurons exposed to transient global ischemia in the rat

Carol K. Petito, Jorge Torres-Munoz, Brenda Roberts, John Paul Olarte, Thaddeus S. Nowak, William A. Pulsinelli

Research output: Contribution to journalArticlepeer-review

130 Scopus citations


Apoptosis is an active, gene-directed process of cell death in which early fragmentation of nuclear DNA precedes morphological changes in the nucleus and, later, in the cytoplasm. In ischemia, biochemical studies have detected oligonucleosomes of apoptosis whereas sequential morphological studies show changes consistent with necrosis rather than apoptosis. To resolve this apparent discrepancy, we subjected rats to 10 minutes of transient forebrain ischemia followed by 1 to 14 days of reperfusion. Parameters evaluated in the CA1 region of the hippocampus included morphology, in situ end labeling (ISEL) of fragmented DNA, and expression of p53. Neurons were indistinguishable from controls at postischemic day 1 but displayed cytoplasmic basophilia or focal condensations at day 2; some neurons were slightly swollen and a few appeared normal. In situ end labeling was absent. At days 3 and 5, approximately 40 to 60% of CA1 neurons had shrunken eosinophilic cytoplasm and pyknotic nuclei, but only half of these were ISEL. By day 14, many of the necrotic neurons had been removed by phagocytes; those remaining retained mild ISEL. Neither p53 protein nor mRNA were identified in control or postischemic brain by in situ hybridization with riboprobes or by northern blot analysis. These results show that DNA fragmentation occurs after the development of delayed neuronal death in CA1 neurons subjected to 10 minutes of global ischemia. They suggest that mechanisms other than apoptosis may mediate the irreversible changes in the CA1 neurons in this model.

Original languageEnglish (US)
Pages (from-to)967-976
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Issue number9
StatePublished - Sep 1997


  • Apoptosis
  • Delayed neuronal death
  • Global ischemia
  • In situ end labeling
  • Necrosis
  • Rat

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism


Dive into the research topics of 'DNA fragmentation follows delayed neuronal death in CA1 neurons exposed to transient global ischemia in the rat'. Together they form a unique fingerprint.

Cite this