DNA damage induced by chloramphenicol and its nitroso derivative: Damage in intact cells

A. A. Yunis, G. K. Arimura, M. Isildar

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We have postulated that the p‐NO2 group of chloramphenicol (CAP) is the structural feature underlying aplastic anemia from this drug. In a series of studies to examine this hypothesis we have demonstrated the toxic nature of the CAP‐reduction intermediate nitroso CAP (NO‐CAP) and its damaging effect on isolated DNA in vitro. The present study was designed to examine the comparative effects of CAP, NO‐CAP, and thiamphenicol (TAP) on the integrity of DNA in intact cells. By using the alkaline elution technique of Kohn, DNA damage in the form of single strand breaks could be readily demonstrated in cultured Raji cells and in PHA‐stimulated normal human lymphocytes by small concentrations of NO‐CAP (0.05‐0.1 mM). A small but reproducible effect was observed from large concentrations of CAP (2 mM). In contrast, TAP, lacking the p‐NO2 group, was without effect.

Original languageEnglish (US)
Pages (from-to)77-84
Number of pages8
JournalAmerican Journal of Hematology
Volume24
Issue number1
DOIs
StatePublished - Jan 1987

Keywords

  • nitrosochloramphenicol

ASJC Scopus subject areas

  • Hematology

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