DNA damage induced by chloramphenicol and its nitroso derivative: Damage in intact cells

Adel A Yunis, G. K. Arimura, M. Isildar

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have postulated that the p-NO2 group of chloramphenicol (CAP) is the structural feature underlying aplastic anemia from this drug. In a series of studies to examine this hypothesis we have demonstrated the toxic nature of the CAP-reduction intermediate nitroso CAP (NO-CAP) and its damaging effect on isolated DNA in vitro. The present study was designed to examine the comparative effects of CAP, NO-CAP, and thiamphenicol (TAP) on the integrity of DNA in intact cells. By using the alkaline elution technique of Kohn, DNA damage in the form of single strand breaks could be readily demonstrated in cultural Raji cells and in PHA-stimulated normal human lymphocytes by small concentrations of NO-CAP (0.05-0.1 mM). A small but reproducible effect was observed from large concentrations of CAP (2 mM). In contrast, TAP, lacking the p-NO2 group, was without effect.

Original languageEnglish (US)
Pages (from-to)77-84
Number of pages8
JournalAmerican Journal of Hematology
Volume24
Issue number1
StatePublished - 1987

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Chloramphenicol
DNA Damage
Thiamphenicol
Aplastic Anemia
Poisons
DNA
Lymphocytes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Hematology

Cite this

DNA damage induced by chloramphenicol and its nitroso derivative : Damage in intact cells. / Yunis, Adel A; Arimura, G. K.; Isildar, M.

In: American Journal of Hematology, Vol. 24, No. 1, 1987, p. 77-84.

Research output: Contribution to journalArticle

Yunis, Adel A ; Arimura, G. K. ; Isildar, M. / DNA damage induced by chloramphenicol and its nitroso derivative : Damage in intact cells. In: American Journal of Hematology. 1987 ; Vol. 24, No. 1. pp. 77-84.
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