TY - JOUR
T1 - DNA copy number losses at 1p32-pter in monozygotic twins concordant for breast cancer
AU - El-Rifai, Wa'El
AU - Tarmo, Livia
AU - Hemmer, Samuli
AU - Försti, Asta
AU - Pedersen, Nancy
AU - Lichtenstein, Paul
AU - Ahlbom, Anders
AU - Söderberg, Magnus
AU - Knuutila, Sakari
AU - Hemminki, Kari
N1 - Funding Information:
We thank Riitta Partanen for technical assistance. This work was supported in part by the Swedish Cancer Fund in Sweden, and the Finnish Cancer Society and Sigrid Jusélius Foundation in Finland.
PY - 1999/7/15
Y1 - 1999/7/15
N2 - To find similarities that may possibly indicate novel mutations, we performed comparative genomic hybridization (CGH) analysis following degenerate oligonucleotide primed polymerase chain reaction (PCR) for DNA obtained from unique material of breast cancer that developed in monozygotic twin-pairs. Polymerase chain reaction amplification was successful in 12 samples for 11 patients, including 3 pairs. Six samples exhibited DNA copy number changes. Gains (76%) were more frequent than losses (24%). Gains or high-level amplifications in 8q were present in all but 1 of the abnormal cases. Frequent gains were detected with a minimal common overlapping region at 5p (4 cases), at 1q25-qter (3 cases), and at 20q12-qter (2 cases). The most frequent loss, detected in half of the abnormal cases, was at 1p32-pter. One twin-pair showed similar changes in 4 chromosomal locations involving loss of 1p32-pter and gains in 1q25-qter, 5, and 8q. Copyright (C) 1999 Elsevier Science Inc.
AB - To find similarities that may possibly indicate novel mutations, we performed comparative genomic hybridization (CGH) analysis following degenerate oligonucleotide primed polymerase chain reaction (PCR) for DNA obtained from unique material of breast cancer that developed in monozygotic twin-pairs. Polymerase chain reaction amplification was successful in 12 samples for 11 patients, including 3 pairs. Six samples exhibited DNA copy number changes. Gains (76%) were more frequent than losses (24%). Gains or high-level amplifications in 8q were present in all but 1 of the abnormal cases. Frequent gains were detected with a minimal common overlapping region at 5p (4 cases), at 1q25-qter (3 cases), and at 20q12-qter (2 cases). The most frequent loss, detected in half of the abnormal cases, was at 1p32-pter. One twin-pair showed similar changes in 4 chromosomal locations involving loss of 1p32-pter and gains in 1q25-qter, 5, and 8q. Copyright (C) 1999 Elsevier Science Inc.
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U2 - 10.1016/S0165-4608(98)00274-X
DO - 10.1016/S0165-4608(98)00274-X
M3 - Article
C2 - 10686947
AN - SCOPUS:0033054809
VL - 112
SP - 169
EP - 172
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
SN - 0165-4608
IS - 2
ER -