DNA copy number losses at 1p32-pter in monozygotic twins concordant for breast cancer

Wa'El El-Rifai, Livia Tarmo, Samuli Hemmer, Asta Försti, Nancy Pedersen, Paul Lichtenstein, Anders Ahlbom, Magnus Söderberg, Sakari Knuutila, Kari Hemminki

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7 Scopus citations

Abstract

To find similarities that may possibly indicate novel mutations, we performed comparative genomic hybridization (CGH) analysis following degenerate oligonucleotide primed polymerase chain reaction (PCR) for DNA obtained from unique material of breast cancer that developed in monozygotic twin-pairs. Polymerase chain reaction amplification was successful in 12 samples for 11 patients, including 3 pairs. Six samples exhibited DNA copy number changes. Gains (76%) were more frequent than losses (24%). Gains or high-level amplifications in 8q were present in all but 1 of the abnormal cases. Frequent gains were detected with a minimal common overlapping region at 5p (4 cases), at 1q25-qter (3 cases), and at 20q12-qter (2 cases). The most frequent loss, detected in half of the abnormal cases, was at 1p32-pter. One twin-pair showed similar changes in 4 chromosomal locations involving loss of 1p32-pter and gains in 1q25-qter, 5, and 8q. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)169-172
Number of pages4
JournalCancer Genetics and Cytogenetics
Volume112
Issue number2
DOIs
StatePublished - Jul 15 1999
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

El-Rifai, WE., Tarmo, L., Hemmer, S., Försti, A., Pedersen, N., Lichtenstein, P., Ahlbom, A., Söderberg, M., Knuutila, S., & Hemminki, K. (1999). DNA copy number losses at 1p32-pter in monozygotic twins concordant for breast cancer. Cancer Genetics and Cytogenetics, 112(2), 169-172. https://doi.org/10.1016/S0165-4608(98)00274-X