Abstract
The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.
| Original language | English |
|---|---|
| Pages (from-to) | 453-456 |
| Number of pages | 4 |
| Journal | Science |
| Volume | 255 |
| Issue number | 5043 |
| State | Published - Jan 24 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
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Cite this
DNA binding activity of recombinant SRY from normal males and XY females. / Harley, Vincent R.; Jackson, David I.; Hextall, Patrick J.; Hawkins, J. Ross; Berkovitz, Gary; Sockanathan, Shanthini; Lovell-Badge, Robin; Goodfellow, Peter N.
In: Science, Vol. 255, No. 5043, 24.01.1992, p. 453-456.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA binding activity of recombinant SRY from normal males and XY females
AU - Harley, Vincent R.
AU - Jackson, David I.
AU - Hextall, Patrick J.
AU - Hawkins, J. Ross
AU - Berkovitz, Gary
AU - Sockanathan, Shanthini
AU - Lovell-Badge, Robin
AU - Goodfellow, Peter N.
PY - 1992/1/24
Y1 - 1992/1/24
N2 - The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.
AB - The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.
UR - http://www.scopus.com/inward/record.url?scp=0026509548&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026509548&partnerID=8YFLogxK
M3 - Article
C2 - 1734522
AN - SCOPUS:0026509548
VL - 255
SP - 453
EP - 456
JO - Science
JF - Science
SN - 0036-8075
IS - 5043
ER -