DNA binding activity of recombinant SRY from normal males and XY females

Vincent R. Harley, David I. Jackson, Patrick J. Hextall, J. Ross Hawkins, Gary Berkovitz, Shanthini Sockanathan, Robin Lovell-Badge, Peter N. Goodfellow

Research output: Contribution to journalArticle

350 Citations (Scopus)

Abstract

The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.

Original languageEnglish
Pages (from-to)453-456
Number of pages4
JournalScience
Volume255
Issue number5043
StatePublished - Jan 24 1992
Externally publishedYes

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DNA
DNA-Binding Proteins
Mutant Proteins
Point Mutation
Recombinant Proteins
Testis
T-Lymphocytes
Genes
XY female 46
Proteins

ASJC Scopus subject areas

  • General

Cite this

Harley, V. R., Jackson, D. I., Hextall, P. J., Hawkins, J. R., Berkovitz, G., Sockanathan, S., ... Goodfellow, P. N. (1992). DNA binding activity of recombinant SRY from normal males and XY females. Science, 255(5043), 453-456.

DNA binding activity of recombinant SRY from normal males and XY females. / Harley, Vincent R.; Jackson, David I.; Hextall, Patrick J.; Hawkins, J. Ross; Berkovitz, Gary; Sockanathan, Shanthini; Lovell-Badge, Robin; Goodfellow, Peter N.

In: Science, Vol. 255, No. 5043, 24.01.1992, p. 453-456.

Research output: Contribution to journalArticle

Harley, VR, Jackson, DI, Hextall, PJ, Hawkins, JR, Berkovitz, G, Sockanathan, S, Lovell-Badge, R & Goodfellow, PN 1992, 'DNA binding activity of recombinant SRY from normal males and XY females', Science, vol. 255, no. 5043, pp. 453-456.
Harley VR, Jackson DI, Hextall PJ, Hawkins JR, Berkovitz G, Sockanathan S et al. DNA binding activity of recombinant SRY from normal males and XY females. Science. 1992 Jan 24;255(5043):453-456.
Harley, Vincent R. ; Jackson, David I. ; Hextall, Patrick J. ; Hawkins, J. Ross ; Berkovitz, Gary ; Sockanathan, Shanthini ; Lovell-Badge, Robin ; Goodfellow, Peter N. / DNA binding activity of recombinant SRY from normal males and XY females. In: Science. 1992 ; Vol. 255, No. 5043. pp. 453-456.
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