Abstract
The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 453-456 |
| Number of pages | 4 |
| Journal | Science |
| Volume | 255 |
| Issue number | 5043 |
| DOIs | |
| State | Published - Jan 1 1992 |
| Externally published | Yes |
ASJC Scopus subject areas
- General
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Cite this
Harley, V. R., Jackson, D. I., Hextall, P. J., Hawkins, J. R., Berkovitz, G. D., Sockanathan, S., Lovell-Badge, R., & Goodfellow, P. N. (1992). DNA binding activity of recombinant SRY from normal males and XY females. Science, 255(5043), 453-456. https://doi.org/10.1126/science.1734522