DNA binding activity of recombinant SRY from normal males and XY females

Vincent R. Harley; David I. Jackson; Parick J. Hextall; J. Ross Hawkins; Gay D. Berkovitz; Shanthini Sockanathan; Robin Lovell-Badge; Peter N. Goodfellow

doi:10.1126/science.1734522

DNA binding activity of recombinant SRY from normal males and XY females

Vincent R. Harley, David I. Jackson, Parick J. Hextall, J. Ross Hawkins, Gay D. Berkovitz, Shanthini Sockanathan, Robin Lovell-Badge, Peter N. Goodfellow

Research output: Contribution to journal › Article › peer-review

360 Scopus citations

Abstract

The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.

Original language	English (US)
Pages (from-to)	453-456
Number of pages	4
Journal	Science
Volume	255
Issue number	5043
DOIs	https://doi.org/10.1126/science.1734522
State	Published - Jan 1 1992
Externally published	Yes

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title = "DNA binding activity of recombinant SRY from normal males and XY females",

abstract = "The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.",

author = "Harley, {Vincent R.} and Jackson, {David I.} and Hextall, {Parick J.} and Hawkins, {J. Ross} and Berkovitz, {Gay D.} and Shanthini Sockanathan and Robin Lovell-Badge and Goodfellow, {Peter N.}",

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T1 - DNA binding activity of recombinant SRY from normal males and XY females

AU - Harley, Vincent R.

AU - Jackson, David I.

AU - Hextall, Parick J.

AU - Hawkins, J. Ross

AU - Berkovitz, Gay D.

AU - Sockanathan, Shanthini

AU - Lovell-Badge, Robin

AU - Goodfellow, Peter N.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.

AB - The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence ACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.

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