Diversity of cytochrome P450 2 family genes in non-mammalian vertebrates

Marjorie F Oleksiak, M. E. Hahn, J. J. Stegeman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The diversity and multiplicity of CYP2 genes in early diverging vertebrates were assessed using RT-PCR to identify sequences in an elasmobranch (dogfish: Squalus acanthias), a teleost (scup: Stenotomus chrysops), an amphibian (mudpuppy: Necturus maculosus), a reptile (turtle: Chrysemys picta) and a bird (chicken: Gallus gallus). Sixteen novel sequences were amplified using total RNA from liver of dogfish, turtle, mudpuppy and chicken. Two turtle sequences, four mudpuppy sequences and one dogfish sequence clustered with frog CYP2Q and chicken CYP2H genes. One turtle sequence grouped with CYP2E. A new chicken CYP2 clustered with rat CYP2B, CYP2G and CYP2A and trout CYP2M. A dogfish sequence and a turtle sequence grouped with CYP2D and CYP2K. Eight additional sequences were amplified from scup liver, intestine, brain and heart; these all grouped in a CYP2J/2N/2P clade. One sequence, prov. scup CYP2N3 (but possibly a CYP2N1 orthologue), was isolated from scup heart, gut, liver and brain. In the brain, cDNAs were amplified from the olfactory lobe, medulla, infundibulum and pituitary. One sequence was most highly expressed in the pituitary, where it may function in some aspect of hormonal control. Distinct CYP2P-related sequences were isolated from scup liver (prov. CYP2P4) and heart (prov. CYP2P5). Addition-al sequences from scup heart and brain RNA also grouped within this clade but could not be assigned to a subfamily. These results greatly expand the number of non-mammalian CYP2 genes known. Non-mammalian sequences may help to define CYP2 subfamily relationships and to identify conserved groups and functions. [Support: Sea Grant NA46RG0470, R/P60.] Copyright (C) 2000 .

Original languageEnglish
Pages (from-to)63-64
Number of pages2
JournalMarine Environmental Research
Volume50
Issue number1-5
StatePublished - Nov 14 2000
Externally publishedYes

Fingerprint

turtle
cytochrome P-450
Liver
turtles
cytochrome
Brain
vertebrate
Genes
vertebrates
brain
heart
gene
chickens
RNA
liver
genes
Chrysops
Birds
Chrysemys picta
Squalus acanthias

ASJC Scopus subject areas

  • Aquatic Science
  • Oceanography
  • Environmental Science(all)

Cite this

Diversity of cytochrome P450 2 family genes in non-mammalian vertebrates. / Oleksiak, Marjorie F; Hahn, M. E.; Stegeman, J. J.

In: Marine Environmental Research, Vol. 50, No. 1-5, 14.11.2000, p. 63-64.

Research output: Contribution to journalArticle

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