TY - JOUR
T1 - Distribution of type I, II, III and v in the pepsin solubilized collagens in bovine menisci
AU - Cheung, Herman S.
N1 - Funding Information:
*From the Division of Rheumatology. Dcpiirtment 01 Medicine. Medical College of Wisconsin. 8700 West Wisconsin Avenue. Milwaukee. Wisconsin 53226. Supported in part by USPHS grant AM26062 and Research Career Development Award AM 1065. **Professor of Medicine. Associate Profcssor of Biochemistry and Orthopedic Surgery.
PY - 1987
Y1 - 1987
N2 - The inner one-third (IM) of both lateral and medial menisci resembles hyaline cartilage, both in gross appearance and histological examination, while the outer two-thirds (OM) is fibrocartilaginous in appearance. Collagen was extracted with pepsin, purified with anion and cation exchange column chromatographies and examined by differential salt precipitation, cyanogen bromide-peptide analysis and SDS gel electrophoresis. IM constitutes approximately 10% of the wet weight of whole meniscus, is made up of 70% collagen of which 34% is pepsin soluble. IM is composed of 60% type II and 40% type I collagen. OM is made up of 80% collagen of which 17% is pepsin soluble. The predominant collagen of OM is type I with a trace amount of types III and V (less than 1%.
AB - The inner one-third (IM) of both lateral and medial menisci resembles hyaline cartilage, both in gross appearance and histological examination, while the outer two-thirds (OM) is fibrocartilaginous in appearance. Collagen was extracted with pepsin, purified with anion and cation exchange column chromatographies and examined by differential salt precipitation, cyanogen bromide-peptide analysis and SDS gel electrophoresis. IM constitutes approximately 10% of the wet weight of whole meniscus, is made up of 70% collagen of which 34% is pepsin soluble. IM is composed of 60% type II and 40% type I collagen. OM is made up of 80% collagen of which 17% is pepsin soluble. The predominant collagen of OM is type I with a trace amount of types III and V (less than 1%.
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U2 - 10.3109/03008208709005619
DO - 10.3109/03008208709005619
M3 - Article
C2 - 3132349
AN - SCOPUS:0023477285
VL - 16
SP - 343
EP - 356
JO - Connective Tissue Research
JF - Connective Tissue Research
SN - 0300-8207
IS - 4
ER -