Distribution of type I, II, III and v in the pepsin solubilized collagens in bovine menisci

Herman S. Cheung

doi:10.3109/03008208709005619

Distribution of type I, II, III and v in the pepsin solubilized collagens in bovine menisci

Herman S. Cheung

Research output: Contribution to journal › Article › peer-review

149 Scopus citations

Abstract

The inner one-third (IM) of both lateral and medial menisci resembles hyaline cartilage, both in gross appearance and histological examination, while the outer two-thirds (OM) is fibrocartilaginous in appearance. Collagen was extracted with pepsin, purified with anion and cation exchange column chromatographies and examined by differential salt precipitation, cyanogen bromide-peptide analysis and SDS gel electrophoresis. IM constitutes approximately 10% of the wet weight of whole meniscus, is made up of 70% collagen of which 34% is pepsin soluble. IM is composed of 60% type II and 40% type I collagen. OM is made up of 80% collagen of which 17% is pepsin soluble. The predominant collagen of OM is type I with a trace amount of types III and V (less than 1%.

Original language	English (US)
Pages (from-to)	343-356
Number of pages	14
Journal	Connective Tissue Research
Volume	16
Issue number	4
DOIs	https://doi.org/10.3109/03008208709005619
State	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Rheumatology
Biochemistry
Orthopedics and Sports Medicine
Molecular Biology
Cell Biology

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@article{ffb45383705d419f82780cd81d162698,

title = "Distribution of type I, II, III and v in the pepsin solubilized collagens in bovine menisci",

abstract = "The inner one-third (IM) of both lateral and medial menisci resembles hyaline cartilage, both in gross appearance and histological examination, while the outer two-thirds (OM) is fibrocartilaginous in appearance. Collagen was extracted with pepsin, purified with anion and cation exchange column chromatographies and examined by differential salt precipitation, cyanogen bromide-peptide analysis and SDS gel electrophoresis. IM constitutes approximately 10% of the wet weight of whole meniscus, is made up of 70% collagen of which 34% is pepsin soluble. IM is composed of 60% type II and 40% type I collagen. OM is made up of 80% collagen of which 17% is pepsin soluble. The predominant collagen of OM is type I with a trace amount of types III and V (less than 1%.",

author = "Cheung, {Herman S.}",

note = "Funding Information: *From the Division of Rheumatology. Dcpiirtment 01 Medicine. Medical College of Wisconsin. 8700 West Wisconsin Avenue. Milwaukee. Wisconsin 53226. Supported in part by USPHS grant AM26062 and Research Career Development Award AM 1065. **Professor of Medicine. Associate Profcssor of Biochemistry and Orthopedic Surgery.",

year = "1987",

doi = "10.3109/03008208709005619",

language = "English (US)",

volume = "16",

pages = "343--356",

journal = "Connective Tissue Research",

issn = "0300-8207",

publisher = "Informa Healthcare",

number = "4",

}

TY - JOUR

T1 - Distribution of type I, II, III and v in the pepsin solubilized collagens in bovine menisci

AU - Cheung, Herman S.

N1 - Funding Information: *From the Division of Rheumatology. Dcpiirtment 01 Medicine. Medical College of Wisconsin. 8700 West Wisconsin Avenue. Milwaukee. Wisconsin 53226. Supported in part by USPHS grant AM26062 and Research Career Development Award AM 1065. **Professor of Medicine. Associate Profcssor of Biochemistry and Orthopedic Surgery.

PY - 1987

Y1 - 1987

N2 - The inner one-third (IM) of both lateral and medial menisci resembles hyaline cartilage, both in gross appearance and histological examination, while the outer two-thirds (OM) is fibrocartilaginous in appearance. Collagen was extracted with pepsin, purified with anion and cation exchange column chromatographies and examined by differential salt precipitation, cyanogen bromide-peptide analysis and SDS gel electrophoresis. IM constitutes approximately 10% of the wet weight of whole meniscus, is made up of 70% collagen of which 34% is pepsin soluble. IM is composed of 60% type II and 40% type I collagen. OM is made up of 80% collagen of which 17% is pepsin soluble. The predominant collagen of OM is type I with a trace amount of types III and V (less than 1%.

AB - The inner one-third (IM) of both lateral and medial menisci resembles hyaline cartilage, both in gross appearance and histological examination, while the outer two-thirds (OM) is fibrocartilaginous in appearance. Collagen was extracted with pepsin, purified with anion and cation exchange column chromatographies and examined by differential salt precipitation, cyanogen bromide-peptide analysis and SDS gel electrophoresis. IM constitutes approximately 10% of the wet weight of whole meniscus, is made up of 70% collagen of which 34% is pepsin soluble. IM is composed of 60% type II and 40% type I collagen. OM is made up of 80% collagen of which 17% is pepsin soluble. The predominant collagen of OM is type I with a trace amount of types III and V (less than 1%.

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